Abstract:
:Mild alkaline extraction of native poly-beta-hydroxybutyrate (PHB) granules results in the solubilization of a protein fraction. Both the solubilized protein fraction and the extracted granules are essentially devoid of PHB synthetase activity unless recombined. The protein fraction has been separated by chromatography into two components (A-I and A-II). A-I but not A-II can be recombined with extracted granules to give rise to PHB synthetase activity. Extracted granules no longer require pretreatment with activator or trypsin but are directly susceptible to hydrolysis by Rhodospirillum rubrum depolymerase. Addition of A-II or A-I prevents the direct hydrolysis by depolymerase. The inhibition is reversed by activator or trypsin. We conclude that native granules are associated with a protein inhibitor which prevents the hydrolysis of PHB by depolymerase unless the protein is destroyed by trypsin, removed by alkaline extraction, or modified by activator.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Griebel RJ,Merrick JMdoi
10.1128/JB.108.2.782-789.1971subject
Has Abstractpub_date
1971-11-01 00:00:00pages
782-9issue
2eissn
0021-9193issn
1098-5530journal_volume
108pub_type
杂志文章abstract::Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.2.946-947.1977
更新日期:1977-05-01 00:00:00
abstract::Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1197-1204.1988
更新日期:1988-03-01 00:00:00
abstract::H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00131-07
更新日期:2007-07-01 00:00:00
abstract::A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.17.4742-4745.1998
更新日期:1998-09-01 00:00:00
abstract::Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.900-910.1978
更新日期:1978-09-01 00:00:00
abstract::The participation of tyramine oxidase in the regulation of arylsulfatase synthesis in Salmonella typhimurium was studied. Arylsulfatase synthesis was repressed by inorganic sulfate, cysteine, methionine, or taurine. This repression was relieved by tyramine, octopamine, or dopamine, which induced tyramine oxidase synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.796-802.1981
更新日期:1981-02-01 00:00:00
abstract::Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6298-6302.1992
更新日期:1992-10-01 00:00:00
abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.821-824.2000
更新日期:2000-02-01 00:00:00
abstract::Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3762-3778.2005
更新日期:2005-06-01 00:00:00
abstract::Escherichia coli mutant N4316 is temperature sensitive and exhibits temperature-dependent suppression. These phenotypes are due to separate genes, as shown by reversion and mapping studies. The suppressor mutation was mapped and lies near argF. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1141-1143.1977
更新日期:1977-02-01 00:00:00
abstract::At some time during sporulation development, cells of Bacillus subtilis develop a commitment to continue sporulation even after addition of or dilution into a fresh nutrient. The extent of commitment was measured by the titer of spores produced at the time at which the original culture sporulated maximally. Since newl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.901-907.1977
更新日期:1977-02-01 00:00:00
abstract::Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2360-2366.1990
更新日期:1990-05-01 00:00:00
abstract::We developed a procedure for isolating membrane vesicles from the homolactic fermentative bacterium Streptococcus cremoris. The membrane vesicles were shown to have a right-side-out orientation by freeze-etch electron microscopy and to be free of cytoplasmic constituents. The membrane vesicles retained their functiona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.733-738.1982
更新日期:1982-02-01 00:00:00
abstract::The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7659-7669.2004
更新日期:2004-11-01 00:00:00
abstract::Small plasmids which replicate in both Escherichia coli and Clostridium perfringens were made by recombining E. coli plasmid pBR322 with three different small (less than 4 kilobases) plasmids native to C. perfringens. Subsequently, two homologous, though distinct, tetracycline resistance determinants (tet) from other ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.465-471.1984
更新日期:1984-08-01 00:00:00
abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.3228-3231.1993
更新日期:1993-05-01 00:00:00
abstract::Caulobacter crescentus initiates a single round of DNA replication during each cell cycle. Following the initiation of DNA replication, the essential CckA histidine kinase is activated by phosphorylation, which (via the ChpT phosphotransferase) enables the phosphorylation and activation of the CtrA global regulator. C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00468-10
更新日期:2010-08-01 00:00:00
abstract::Mycoplasmas isolated from simian (Cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain Navel isolated from man 15 years ago. The simian and human Navel strains comprised a single serogroup, distinct from the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.439-445.1971
更新日期:1971-10-01 00:00:00
abstract::The position of the termination codon in lacZX90 was determined by isolation of a lac+ revertant. Lysine was found to replace tyrosine at position 1,012 of beta-galactosidase, indicating that X90 protein lacked the carboxyl-terminal 10 residues. A heat- and urea-sensitive hybrid enzyme was formed in vivo when supC, wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.694-697.1981
更新日期:1981-08-01 00:00:00
abstract::Riemerella anatipestifer is a Gram-negative, rod-shaped bacterium associated with epizootic infections in poultry. R. anatipestifer strain RA-YM, belonging to the serotype 1 prevalent in China, is a clinically isolated strain with high-level virulence. Here, we report the first genome sequence of this species. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01445-10
更新日期:2011-03-01 00:00:00
abstract::Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01578-08
更新日期:2009-04-01 00:00:00
abstract::Davis, Nour A. (University of Lagos Medical School, Lagos, Nigeria), and G. H. G. Davis. Ecology of nasal staphylococci. J. Bacteriol. 89:1163-1168. 1965.-The rate of nasal carriage of Staphylococcus aureus in Nigerian adults (46%) approximates that found in other countries. The rate in infants under 12 months was ca....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.5.1163-1168.1965
更新日期:1965-05-01 00:00:00
abstract::A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4655-4666.1989
更新日期:1989-09-01 00:00:00
abstract::Duerre, John A. (University of North Dakota, Grand Forks), and Patrick J. Buckley. Pigment production from tryptophan by an Achromobacter species. J. Bacteriol. 90:1686-1691. 1965.-A microorganism was isolated from the soil near the University of North Dakota. Biochemical and morphological characteristics indicated th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.6.1686-1691.1965
更新日期:1965-12-01 00:00:00
abstract::Rickettsia japonica strain YH, isolated in 1984 in Japan, is the type strain of R. japonica, the tick-borne agent of Japanese spotted fever. Here, we report the 1.33-Mb genome of this rickettsial species. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01928-12
更新日期:2012-12-01 00:00:00
abstract::Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.23.6437-6447.2002
更新日期:2002-12-01 00:00:00
abstract::The synthesis of cytosine deaminase in Salmonella typhimurium is repressed by pyrimidines. This repression is mediated by both a uridine and a cytidine compound, indicating a distinct difference in the regulation of synthesis of cytosine deaminase from the regulation of the de novo pyrimidine pathway enzymes. A salvag...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.1171-1174.1982
更新日期:1982-03-01 00:00:00
abstract::Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.1065-1070.1964
更新日期:1964-10-01 00:00:00
abstract::A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00625-12
更新日期:2012-07-01 00:00:00
abstract::A novel phenotype is described for Escherichia coli K-12 carrying the prlA4 allele determining a membrane component of the protein export mechanism. It is manifest as transformation deficiency for plasmids containing the cloned group C streptococcal streptokinase gene, skc. Streptokinase plasmid mutations relieving th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2202-2208.1989
更新日期:1989-04-01 00:00:00