Abstract:
:H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC/XylS family. DNase I footprinting revealed that the activator has two binding sites upstream of the pilus promoter cfaAp. One site extends from positions -23 to -56, and the other extends from positions -73 to -103 (numbering relative to the transcription start site of cfaAp). Additional CfaD binding sites were predicted within the genome of H10407 by computational analysis. Two of these sites lie upstream of a previously uncharacterized gene, cexE. In vitro DNase I footprinting confirmed that both sites are genuine binding sites, and cexEp::lacZ reporters demonstrated that CfaD is required for the expression of cexE in vivo. The amino terminus of CexE contains a secretory signal peptide that is removed during translocation across the cytoplasmic membrane through the general secretory pathway. These studies suggest that CexE may be a novel ETEC virulence factor because its expression is controlled by the virulence regulator CfaD, and its distribution is restricted to ETEC.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Pilonieta MC,Bodero MD,Munson GPdoi
10.1128/JB.00131-07subject
Has Abstractpub_date
2007-07-01 00:00:00pages
5060-7issue
14eissn
0021-9193issn
1098-5530pii
JB.00131-07journal_volume
189pub_type
杂志文章abstract::The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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doi:10.1128/JB.151.3.1397-1402.1982
更新日期:1982-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1977-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1967-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.150.3.1016-1023.1982
更新日期:1982-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.182.3.749-757.2000
更新日期:2000-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.16.5205-5215.1993
更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.171.6.2919-2924.1989
更新日期:1989-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.562-563.1975
更新日期:1975-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.963-965.1967
更新日期:1967-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.2.570-577.1972
更新日期:1972-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.135-141.1967
更新日期:1967-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.517-522.1986
更新日期:1986-02-01 00:00:00
abstract::Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.4056-4063.1990
更新日期:1990-07-01 00:00:00