The groESL operon of Agrobacterium tumefaciens: evidence for heat shock-dependent mRNA cleavage.

abstract：:Insertion-duplication mutagenesis was used to generate mutants of Streptococcus pneumoniae that produced truncated forms of PspA (pneumococcal surface protein A). The truncated products, representing from 20 to 80% of the complete PspA molecule, were all secreted from the cell and could be detected in unconcentrated c...

journal_title：Journal of bacteriology

authors： Yother J,Handsome GL,Briles DE

更新日期：1992-01-01 00:00:00

abstract：:Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...

journal_title：Journal of bacteriology

authors： Chen JH,Hsu WB,Hwang JL

更新日期：1998-10-01 00:00:00

abstract：:The Tm of Bacillus stearothermophilus 5S ribonucleic acid (RNA) is 1.5 +/- 0.5 C higher than that of 5S RNAs from B. subtilis and Escherichia coli. Melting in 50% methanol and in formaldehyde indicate that both base stacking and helical regions are involved in the slightly increased thermal stability of B. stearotherm...

journal_title：Journal of bacteriology

authors： Varricchio F,Marotta CA

更新日期：1976-03-01 00:00:00

abstract：:The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...

journal_title：Journal of bacteriology

authors： Yang J,Ritchey M,Yoshida Y,Bush CA,Cisar JO

更新日期：2009-03-01 00:00:00

abstract：:The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...

journal_title：Journal of bacteriology

authors： Vimr ER,Bergstrom R,Steenbergen SM,Boulnois G,Roberts I

更新日期：1992-08-01 00:00:00

abstract：:This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...

journal_title：Journal of bacteriology

authors： Vakharia H,German GJ,Misra R

更新日期：2001-12-01 00:00:00

abstract：:We have previously identified three sites, named sbi, ihfA, and sbyA, specifically recognized or bound by the TraI, IHF, and TraY proteins, respectively; these sites are involved in nicking at the origin of transfer, oriT, of plasmid R100. In the region next to these sites, there exists the sbm region, which consists ...

journal_title：Journal of bacteriology

authors： Abo T,Ohtsubo E

更新日期：1995-08-01 00:00:00

abstract：:The importance of proton movements was assessed in the moderate halophile Vibrio costicola. When anaerobic cells in acidic buffer (pH 6.5) were given an O2 pulse, protons were extruded regardless of the presence of Na+. At pH 8.5, however, V. costicola produced an acidic response to an O2 pulse in the absence of Na+ a...

journal_title：Journal of bacteriology

authors： Hamaide F,Kushner DJ,Sprott GD

更新日期：1985-02-01 00:00:00

abstract：:The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was clon...

journal_title：Journal of bacteriology

authors： Muryoi N,Sato M,Kaneko S,Kawahara H,Obata H,Yaish MW,Griffith M,Glick BR

更新日期：2004-09-01 00:00:00

abstract：:Coxiella burnetii, the etiological agent of acute and chronic Q fever in humans, is a naturally intracellular pathogen that directs the formation of an acidic Coxiella-containing vacuole (CCV) derived from the host lysosomal network. Central to its pathogenesis is a specialized type IVB secretion system (T4SS) that de...

journal_title：Journal of bacteriology

authors： Weber MM,Chen C,Rowin K,Mertens K,Galvan G,Zhi H,Dealing CM,Roman VA,Banga S,Tan Y,Luo ZQ,Samuel JE

更新日期：2013-09-01 00:00:00

abstract：:The pelA and pelC genes from Erwinia chrysanthemi EC16 were sequenced and overexpressed in Escherichia coli cells. These genes and two others from the same strain that were characterized previously encode catalytically related pectate lyase proteins that are involved with the maceration and soft-rotting of plant tissu...

journal_title：Journal of bacteriology

authors： Tamaki SJ,Gold S,Robeson M,Manulis S,Keen NT

更新日期：1988-08-01 00:00:00

abstract：:Saz, Arthur K. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Dolores L. Lowery, and Leah J. Jackson. Staphylococcal penicillinase. I. Inhibition and stimulation of activity. J. Bacteriol. 82:298-304. 1961.-The penicillinase extracted from a penicillin-resistant strain of Staphylococcus aureus...

journal_title：Journal of bacteriology

authors： SAZ AK,LOWERY DL,JACKSON LJ

更新日期：1961-08-01 00:00:00

abstract：:Anabaena variabilis ATCC 29413 has one Mo nitrogenase that is made under oxic growth conditions in specialized cells called heterocysts and a second Mo nitrogenase that is made only under anoxic conditions in vegetative cells. The two large nif gene clusters responsible for these two nitrogenases are under the control...

journal_title：Journal of bacteriology

authors： Vernon SA,Pratte BS,Thiel T

更新日期：2017-01-30 00:00:00

abstract：:Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. T...

journal_title：Journal of bacteriology

authors： Tam JE,Pattee PA

更新日期：1986-11-01 00:00:00

abstract：:Vibrio vulnificus is an estuarine bacterium and potent opportunistic human pathogen. It enters the food chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. Expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by cyclic di-...

journal_title：Journal of bacteriology

authors： Chodur DM,Guo L,Pu M,Bruger E,Fernandez N,Waters C,Rowe-Magnus DA

更新日期：2017-09-05 00:00:00

abstract：:Flagellar phase variation of Salmonella is a phenomenon where two flagellin genes, fliC (phase 1) and fljB (phase 2), are expressed alternately. This is controlled by the inversion of a DNA segment containing the promoter for the fljB gene. The fljB gene constitutes an operon with the fljA gene, which encodes a negati...

journal_title：Journal of bacteriology

authors： Yamamoto S,Kutsukake K

更新日期：2006-02-01 00:00:00

abstract：:A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...

journal_title：Journal of bacteriology

authors： MacNeil D

更新日期：1981-04-01 00:00:00

abstract：:Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...

journal_title：Journal of bacteriology

authors： Doran JL,Page WJ

更新日期：1983-07-01 00:00:00

abstract：:The RpoS sigma factor (σ(S)) is the master regulator of the bacterial response to a variety of stresses. Mutants in rpoS arise in bacterial populations in the absence of stress, probably as a consequence of a subtle balance between self-preservation and nutritional competence. We characterized here one natural rpoS mu...

journal_title：Journal of bacteriology

authors： Monteil V,Kolb A,Mayer C,Hoos S,England P,Norel F

更新日期：2010-12-01 00:00:00

abstract：:A globomycin-resistant mutant of Escherichia coli was found to produce a precursor of the major outer membrane lipoprotein (prolipoprotein), in which the glycine residue at position 14 within the signal peptide was replaced by an aspartic acid residue. The same mutation has been reported by Lin et al. (Proc. Natl. Aca...

journal_title：Journal of bacteriology

authors： Lee N,Yamagata H,Inouye M

更新日期：1983-07-01 00:00:00

abstract：:We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repe...

journal_title：Journal of bacteriology

authors： Mazel D,Houmard J,Castets AM,Tandeau de Marsac N

更新日期：1990-05-01 00:00:00

abstract：:The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

journal_title：Journal of bacteriology

authors： Griffiths MW,Sundaram TK

更新日期：1973-12-01 00:00:00

abstract：:Uracil-DNA glycosylase activity was found in Streptococcus pneumoniae, and the enzyme was partially purified. An ung mutant lacking the activity was obtained by positive selection of cells transformed with a plasmid containing uracil in its DNA. The effects of the ung mutation on mutagenic processes in S. pneumoniae w...

journal_title：Journal of bacteriology

authors： Chen JD,Lacks SA

更新日期：1991-01-01 00:00:00

abstract：:Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...

journal_title：Journal of bacteriology

authors： Kjellin-Stråby K

更新日期：1969-11-01 00:00:00

abstract：:Expression of toxT, the transcription activator of cholera toxin and pilus production in Vibrio cholerae, is the consequence of a complex cascade of regulatory events that culminates in activation of the toxT promoter by TcpP and ToxR, two membrane-localized transcription factors. Both are encoded in operons with gene...

journal_title：Journal of bacteriology

authors： Beck NA,Krukonis ES,DiRita VJ

更新日期：2004-12-01 00:00:00

abstract：:The messenger ribonucleic acid (mRNA) pattern of log-phase and sporulating cells of Bacillus subtilis has been analyzed by deoxyribonucleic acid (DNA)-RNA hybrid studies with the complementary-strand fragments of DNA. Approximately 80% of the mRNA of log-phase and sporulating cells from stages I, III, and IV hybridize...

journal_title：Journal of bacteriology

authors： Sumida-Yasumoto C,Doi RH

更新日期：1974-02-01 00:00:00

abstract：:Lactobacillus plantarum is a lactic acid bacterium (LAB) species commonly used as a probiotic. We have sequenced the genome of Lactobacillus plantarum JDM1, which is a Chinese commercial LAB with several probiotic functions, using a GS 20 system. We recommend that each commercial probiotic strain should undergo comple...

journal_title：Journal of bacteriology

authors： Zhang ZY,Liu C,Zhu YZ,Zhong Y,Zhu YQ,Zheng HJ,Zhao GP,Wang SY,Guo XK

更新日期：2009-08-01 00:00:00

abstract：:Coliphage TuIa, which uses the OmpF protein as a receptor, can adapt by mutation to using instead the OmpC or LamB protein, or both. Most of the phage mutants retained the ability to use the OmpF protein, when present, but one class of mutants lost this ability and could only use the OmpC protein. ...

journal_title：Journal of bacteriology

authors： Moreno F,Wandersman C

更新日期：1980-12-01 00:00:00

abstract：:Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modificat...

journal_title：Journal of bacteriology

authors： Taguchi F,Ogawa Y,Takeuchi K,Suzuki T,Toyoda K,Shiraishi T,Ichinose Y

更新日期：2006-12-01 00:00:00

abstract：:Nitrogenase activities were determined from maximum acetylene reduction rates for mutant strains of Azotobacter vinelandii which are unable to fix N2 in the presence of molybdenum (Nif-) but undergo phenotypic reversal to Nif+ under conditions of Mo deficiency. The system responsible for N2 fixation under these condit...

journal_title：Journal of bacteriology

authors： Bishop PE,Jarlenski DM,Hetherington DR

更新日期：1982-06-01 00:00:00