Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene.

abstract：:Chimeric plasmids, which were useful as cloning vehicles in a Streptococcus sanguis (Challis) host vector system, have been constructed. By using three different strategies of restriction endonuclease digestion and ligation, a deoxyribonucleic acid (DNA) fragment bearing an erythromycin resistance determinant was liga...

journal_title：Journal of bacteriology

authors： Macrina FL,Jones KR,Wood PH

更新日期：1980-09-01 00:00:00

abstract：:PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

journal_title：Journal of bacteriology

authors： Trott AE,Stevens AM

更新日期：2001-01-01 00:00:00

abstract：:Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

journal_title：Journal of bacteriology

authors： Gregory MA,Till R,Smith MC

更新日期：2003-09-01 00:00:00

abstract：:Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

journal_title：Journal of bacteriology

authors： Garcia DL,Dillard JP

更新日期：2008-06-01 00:00:00

abstract：:A group of proteic toxin-antitoxin (TA) cassettes whose representatives are widely distributed among bacterial genomes has been identified. These cassettes occur in chromosomes, plasmids, bacteriophages, and noncomposite transposons, as well as in the SXT conjugative element of Vibrio cholerae. The following four homo...

journal_title：Journal of bacteriology

authors： Dziewit L,Jazurek M,Drewniak L,Baj J,Bartosik D

更新日期：2007-03-01 00:00:00

abstract：:Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each...

journal_title：Journal of bacteriology

authors： Mengucci F,Dardis C,Mongiardini EJ,Althabegoiti MJ,Partridge JD,Kojima S,Homma M,Quelas JI,Lodeiro AR

更新日期：2020-02-11 00:00:00

abstract：:The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...

journal_title：Journal of bacteriology

authors： Römling U,Duchéne M,Essar DW,Galloway D,Guidi-Rontani C,Hill D,Lazdunski A,Miller RV,Schleifer KH,Smith DW

更新日期：1992-01-01 00:00:00

abstract：:Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the...

journal_title：Journal of bacteriology

authors： Toh E,Kurtz HD Jr,Brun YV

更新日期：2008-11-01 00:00:00

abstract：:A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...

journal_title：Journal of bacteriology

authors： Beutin L,Bode L,Ozel M,Stephan R

更新日期：1990-11-01 00:00:00

abstract：:Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a consta...

journal_title：Journal of bacteriology

authors： Girbal L,Vasconcelos I,Soucaille P

更新日期：1994-10-01 00:00:00

abstract：:Mobilizable genomic islands (MGIs) are small genomic islands that are mobilizable by SXT/R391 integrating conjugative elements (ICEs) due to similar origins of transfer. Their site-specific integration and excision are catalyzed by the integrase that they encode, but their conjugative transfer entirely depends upon th...

journal_title：Journal of bacteriology

authors： Daccord A,Mursell M,Poulin-Laprade D,Burrus V

更新日期：2012-11-01 00:00:00

abstract：:The complete nucleotide sequence of the hutH gene, encoding histidine ammonia-lyase (histidase), in Pseudomonas putida ATCC 12633 has been determined from the appropriate portions of the hut region that had been cloned into Escherichia coli. The resulting DNA sequence revealed an open reading frame of 1,530 base pairs...

journal_title：Journal of bacteriology

authors： Consevage MW,Phillips AT

更新日期：1990-05-01 00:00:00

abstract：:Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...

journal_title：Journal of bacteriology

authors： Troiano AJ Jr,Zhang J,Cowan AE,Yu J,Setlow P

更新日期：2015-01-01 00:00:00

abstract：:The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic behavior remain to be elucidated, findings from structural genomic studies have shown that the structure of YfdW, the prote...

journal_title：Journal of bacteriology

authors： Toyota CG,Berthold CL,Gruez A,Jónsson S,Lindqvist Y,Cambillau C,Richards NG

更新日期：2008-04-01 00:00:00

abstract：:The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...

journal_title：Journal of bacteriology

authors： Ishihama A,Kori A,Koshio E,Yamada K,Maeda H,Shimada T,Makinoshima H,Iwata A,Fujita N

更新日期：2014-08-01 00:00:00

abstract：:In Salmonella, ilv-linked rfe genes participate in the biosynthesis of the enterobacterial common antigen (CA) as well as of certain types of O antigen (serogroups C1 and L). rff genes, probably in the same cluster with rfe, are required for CA synthesis (P.H. Mäkelä et al., in preparation). Several Escherichia coli s...

journal_title：Journal of bacteriology

authors： Schmidt G,Mayer H,Mäkelä PH

更新日期：1976-08-01 00:00:00

abstract：:In Escherichia coli, the GlpT transporter, a member of the major facilitator superfamily, moves external glycerol 3-phosphate (G3P) into the cytoplasm in exchange for cytoplasmic phosphate. Study of intact cells showed that both GlpT and HisGlpT, a variant with an N-terminal six-histidine tag, are inhibited (50% inhib...

journal_title：Journal of bacteriology

authors： Fann MC,Busch A,Maloney PC

更新日期：2003-07-01 00:00:00

abstract：:Isolated cell envelopes of a marine bacterium, M.B.3, have been prepared which possess a nonspecific, cation-activated nucleotidase. The cell envelope comprises approximately 35% (dry weight) of the whole cell and contains protein, 60.2%; lipids, 20.7%; hexose, 3.4%; and ribonucleic acid, 4.6%. No deoxyribonucleic aci...

journal_title：Journal of bacteriology

authors： Thompson J,Green ML,Happold FC

更新日期：1969-09-01 00:00:00

abstract：:The photoautotrophic cyanobacterium Anacystis nidulans was used to investigate the membrane transport of branched-chain, neutral amino acids and its dependence on photosynthetic reactions. The uptake of alpha-amino [1-14C]isobutyric acid and L-[1-14C]leucine followed Michaelis, Menten kinetics and resulted in an energ...

journal_title：Journal of bacteriology

authors： Lee-Kaden J,Simonis W

更新日期：1982-07-01 00:00:00

abstract：:A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cros...

journal_title：Journal of bacteriology

authors： Chandler PM,Krishnapillai V

更新日期：1977-05-01 00:00:00

abstract：:LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...

journal_title：Journal of bacteriology

authors： Bowers CW,Lau F,Silhavy TJ

更新日期：2003-10-01 00:00:00

abstract：:Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar. The hmsHFRS genes required for CR binding (Crb(+)) are genetically linked to virulence-associated genes encoding a siderophore uptake system. These genes are contained in a 102-kb chromosomal pgm locus ...

journal_title：Journal of bacteriology

authors： Hare JM,McDonough KA

更新日期：1999-08-01 00:00:00

abstract：:A DNA region carrying lysS, the gene encoding the lysyl-tRNA synthetase, was cloned from the extreme thermophile prokaryote Thermus thermophilus VK-1 and sequenced. The analysis indicated an open reading frame encoding a protein of 492 amino acids. This putative protein has significant homologies to previously sequenc...

journal_title：Journal of bacteriology

authors： Chen J,Brevet A,Lapadat-Tapolsky M,Blanquet S,Plateau P

更新日期：1994-05-01 00:00:00

abstract：:Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

journal_title：Journal of bacteriology

authors： Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

更新日期：2008-08-01 00:00:00

abstract：:Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Fur...

journal_title：Journal of bacteriology

authors： Kusuya Y,Kurokawa K,Ishikawa S,Ogasawara N,Oshima T

更新日期：2011-06-01 00:00:00

abstract：:The general aromatic amino acid permease, AroP, of Escherichia coli is responsible for the active transport of phenylalanine, tyrosine, and tryptophan. A proposed topological model for the AroP permease, consisting of 12 hydrophobic transmembrane spans connected by hydrophilic loops, is very similar to that of the clo...

journal_title：Journal of bacteriology

authors： Cosgriff AJ,Pittard AJ

更新日期：1997-05-01 00:00:00

abstract：:Acclimation to high salt concentrations involves concerted changes in gene expression. For the majority of salt-regulated genes, the mechanism underlying the induction process is not known. The gene ggpS (sll1566), which encodes the glucosylglycerol-phosphate synthase responsible for the synthesis of the compatible so...

journal_title：Journal of bacteriology

authors： Klähn S,Höhne A,Simon E,Hagemann M

更新日期：2010-09-01 00:00:00

abstract：:The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...

journal_title：Journal of bacteriology

authors： Jacobs NJ,Maclosky ER,Conti SF

更新日期：1967-01-01 00:00:00

abstract：:Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...

journal_title：Journal of bacteriology

authors： Liu H,Bouillaut L,Sonenshein AL,Melville SB

更新日期：2013-02-01 00:00:00

abstract：:The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...

journal_title：Journal of bacteriology

authors： Awano N,Xu C,Ke H,Inoue K,Inouye M,Phadtare S

更新日期：2007-08-01 00:00:00