The SXT conjugative element and linear prophage N15 encode toxin-antitoxin-stabilizing systems homologous to the tad-ata module of the Paracoccus aminophilus plasmid pAMI2.

Abstract:

:A group of proteic toxin-antitoxin (TA) cassettes whose representatives are widely distributed among bacterial genomes has been identified. These cassettes occur in chromosomes, plasmids, bacteriophages, and noncomposite transposons, as well as in the SXT conjugative element of Vibrio cholerae. The following four homologous loci were subjected to detailed comparative studies: (i) tad-ata from plasmid pAMI2 of Paracoccus aminophilus (the prototype of this group), (ii) gp49-gp48 from the linear bacteriophage N15 of Escherichia coli, (iii) s045-s044 from SXT, and (iv) Z3230-Z3231 from the genomic island of enterohemorrhagic Escherichia coli O157:H7 strain EDL933. Functional analysis revealed that all but one of these loci (Z3230-Z3231) are able to stabilize heterologous replicons, although the host ranges varied. The TA cassettes analyzed have the following common features: (i) the toxins are encoded by the first gene of each operon; (ii) the antitoxins contain a predicted helix-turn-helix motif of the XRE family; and (iii) the cassettes have two promoters that are different strengths, one which is located upstream of the toxin gene and one which is located upstream of the antitoxin gene. All four toxins tested are functional in E. coli; overexpression of the toxins (in the absence of antitoxin) results in a bacteriostatic effect manifested by elongation of bacterial cells and growth arrest. The toxins have various effects on cell viability, which suggests that they may recognize different intracellular targets. Preliminary data suggest that different cellular proteases are involved in degradation of antitoxins encoded by the loci analyzed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Dziewit L,Jazurek M,Drewniak L,Baj J,Bartosik D

doi

10.1128/JB.01610-06

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

1983-97

issue

5

eissn

0021-9193

issn

1098-5530

pii

JB.01610-06

journal_volume

189

pub_type

杂志文章
  • A functional myo-inositol dehydrogenase gene is required for efficient nitrogen fixation and competitiveness of Sinorhizobium fredii USDA191 to nodulate soybean (Glycine max [L.] Merr.).

    abstract::Inositol derivative compounds provide a nutrient source for soil bacteria that possess the ability to degrade such compounds. Rhizobium strains that are capable of utilizing certain inositol derivatives are better colonizers of their host plants. We have cloned and determined the nucleotide sequence of the myo-inosito...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2595-2604.2001

    authors: Jiang G,Krishnan AH,Kim YW,Wacek TJ,Krishnan HB

    更新日期:2001-04-01 00:00:00

  • Light-induced lysis and carotenogenesis in Myxococcus xanthus.

    abstract::Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535-545. 1966.-Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.535-545.1966

    authors: Burchard RP,Dworkin M

    更新日期:1966-02-01 00:00:00

  • Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine.

    abstract::Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1306-1312.1962

    authors: SATO I,TANAKA T,SAITO K,MITSUHASHI S

    更新日期:1962-06-01 00:00:00

  • Subcellular localization and immunological detection of proteins encoded by the vir locus of Bordetella pertussis.

    abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.14.4288-4296.1991

    authors: Stibitz S,Yang MS

    更新日期:1991-07-01 00:00:00

  • The requirement for pneumococcal MreC and MreD is relieved by inactivation of the gene encoding PBP1a.

    abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05245-11

    authors: Land AD,Winkler ME

    更新日期:2011-08-01 00:00:00

  • Identification of Campylobacter jejuni ATCC 43431-specific genes by whole microbial genome comparisons.

    abstract::This study describes a novel approach to identify unique genomic DNA sequences from the unsequenced strain C. jejuni ATCC 43431 by comparison with the sequenced strain C. jejuni NCTC 11168. A shotgun DNA microarray was constructed by arraying 9,600 individual DNA fragments from a C. jejuni ATCC 43431 genomic library o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.14.4781-4795.2004

    authors: Poly F,Threadgill D,Stintzi A

    更新日期:2004-07-01 00:00:00

  • Whole-genome transcription profiling reveals genes up-regulated by growth on fucose in the human gut bacterium "Roseburia inulinivorans".

    abstract::"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00137-06

    authors: Scott KP,Martin JC,Campbell G,Mayer CD,Flint HJ

    更新日期:2006-06-01 00:00:00

  • Localization of alg, opr, phn, pho, 4.5S RNA, 6S RNA, tox, trp, and xcp genes, rrn operons, and the chromosomal origin on the physical genome map of Pseudomonas aeruginosa PAO.

    abstract::The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.1.327-330.1992

    authors: Römling U,Duchéne M,Essar DW,Galloway D,Guidi-Rontani C,Hill D,Lazdunski A,Miller RV,Schleifer KH,Smith DW

    更新日期:1992-01-01 00:00:00

  • Isolation and preliminary characterization of bacteriophage phi-mu-4.

    abstract::Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Isolation and preliminary characterization of bacteriophage phimu-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage phimu-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.999-1002.1964

    authors: Shafia F,Thompson TL

    更新日期:1964-05-01 00:00:00

  • EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. II. CHARACTERIZATION OF THE LIPASE ACTIVITY.

    abstract::Shah, D. B. (University of Wisconsin, Madison), and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. II. Characterization of the lipase activity. J. Bacteriol. 89:949-953. 1965.-The staphylococcal egg yolk factor was characterized as a lipase. The enzyme had an optimal pH of 7.8, but the optimal pH of stability...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.4.949-953.1965

    authors: SHAH DB,WILSON JB

    更新日期:1965-04-01 00:00:00

  • A naturally occurring gene amplification leading to sulfonamide and trimethoprim resistance in Streptococcus agalactiae.

    abstract::Gene amplifications have been detected as a transitory phenomenon in bacterial cultures. They are predicted to contribute to rapid adaptation by simultaneously increasing the expression of genes clustered on the chromosome. However, genome amplifications have rarely been described in natural isolates. Through DNA arra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01357-07

    authors: Brochet M,Couvé E,Zouine M,Poyart C,Glaser P

    更新日期:2008-01-01 00:00:00

  • Transcriptional analysis of the principal cell division gene, ftsZ, of Mycobacterium tuberculosis.

    abstract::Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.7.2540-2550.2005

    authors: Roy S,Ajitkumar P

    更新日期:2005-04-01 00:00:00

  • Magnesium starvation of Aerobacter aerogenes. II. Rates of nucleic acid synthesis and methods for their measurement.

    abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.345-356.1967

    authors: Kennell D,Kotoulas A

    更新日期:1967-01-01 00:00:00

  • Uracil-DNA glycosylase of Thermoplasma acidophilum directs long-patch base excision repair, which is promoted by deoxynucleoside triphosphates and ATP/ADP, into short-patch repair.

    abstract::Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00233-11

    authors: Moen MN,Knævelsrud I,Haugland GT,Grøsvik K,Birkeland NK,Klungland A,Bjelland S

    更新日期:2011-09-01 00:00:00

  • Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici.

    abstract::Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.370-380.1962

    authors: LYONS AJ Jr,PRIDHAM TG

    更新日期:1962-02-01 00:00:00

  • Ultrastructural, physiological, and cytochemical characterization of cores in group D streptococci.

    abstract::Cores are large, rod-shaped structures that have been found almost exclusively in group D streptococci, measure 0.1 to 0.16 mum in diameter, and extend the width or length of cells. This study has shown that cores are produced in the cells at a reproducible point in early stationary growth after extensive mesosomal fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.445-456.1977

    authors: Coleman SE,Bleiweis AS

    更新日期:1977-01-01 00:00:00

  • Phosphatidylglyceroylalkylamine, a novel phosphoglycolipid precursor in Deinococcus radiodurans.

    abstract::We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.457-462.1991

    authors: Huang Y,Anderson R

    更新日期:1991-01-01 00:00:00

  • Purification and characterization of a cell-associated, soluble mannanase from Bacteroides ovatus.

    abstract::Bacteroides ovatus, a human colonic anaerobe, utilizes the galactomannan guar gum as a sole source of carbohydrate. Previously, we found that none of the galactomannan-degrading enzymes were extracellular, and we characterized an outer membrane mannanase which hydrolyzes the backbone of guar gum to produce large fragm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.2038-2043.1987

    authors: Gherardini FC,Salyers AA

    更新日期:1987-05-01 00:00:00

  • FepA with globular domain deletions lacks activity.

    abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.19.5508-5512.2002

    authors: Vakharia HL,Postle K

    更新日期:2002-10-01 00:00:00

  • Genome sequence of Lactobacillus fructivorans KCTC 3543.

    abstract::Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00075-12

    authors: Nam SH,Choi SH,Kang A,Lee KS,Kim DW,Kim RN,Kim DS,Park HS

    更新日期:2012-04-01 00:00:00

  • Mutations in type I and type IV pilus biosynthetic genes affect twitching motility rates in Xylella fastidiosa.

    abstract::Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00934-07

    authors: De La Fuente L,Burr TJ,Hoch HC

    更新日期:2007-10-01 00:00:00

  • Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281.

    abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4417-4425.1987

    authors: Jin SG,Komari T,Gordon MP,Nester EW

    更新日期:1987-10-01 00:00:00

  • The Escherichia coli mraY gene encoding UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase.

    abstract::Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.3.1021-1026.1991

    authors: Ikeda M,Wachi M,Jung HK,Ishino F,Matsuhashi M

    更新日期:1991-02-01 00:00:00

  • Sociality in Escherichia coli: Enterochelin Is a Private Good at Low Cell Density and Can Be Shared at High Cell Density.

    abstract:UNLABELLED:Many bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of productio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02596-14

    authors: Scholz RL,Greenberg EP

    更新日期:2015-07-01 00:00:00

  • All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis.

    abstract::The seven proteins encoded by the comG operon of Bacillus subtilis exhibit similarity to gene products required for the assembly of type 4 pili and for the secretion of certain proteins in gram-negative bacteria. Although polar transposon insertions in comG result in the loss of transformability and in the failure of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.1.41-45.1998

    authors: Chung YS,Dubnau D

    更新日期:1998-01-01 00:00:00

  • Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.

    abstract::Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.6.1786-1792.1999

    authors: van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

    更新日期:1999-03-01 00:00:00

  • Porin from Rhodopseudomonas sphaeroides.

    abstract::A protein homooligomer was purified from both the cell envelope fractions and the saline extracts of Rhodopseudomonas sphaeroides cells. This oligomer exhibited strong porin activity when reconstituted into proteoliposomes with egg phosphatidylcholine. In the saline extracts of both chemotrophically and phototrophical...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.199-205.1984

    authors: Weckesser J,Zalman LS,Nikaido H

    更新日期:1984-07-01 00:00:00

  • Effect of the proline analogue baikiain on proline metabolism in Salmonella typhimurium.

    abstract::A proline analogue, 4,5-dehydro-l-pipecolic acid (baikiain) induces the formation in Salmonella typhimurium of the two enzymes catalyzing the degradation of proline, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid (P5C) dehydrogenase. The level of induction by 20 mm baikiain is about 10% of the maximum level ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1134-1141.1972

    authors: Dendinger S,Brill WJ

    更新日期:1972-12-01 00:00:00

  • Further electron microscope characterization of spore appendages of Clostridium bifermentans.

    abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.231-238.1968

    authors: Yolton DP,Pope L,Williams MG,Rode LJ

    更新日期:1968-01-01 00:00:00

  • Methylation of inorganic and organic selenium by the bacterial thiopurine methyltransferase.

    abstract::Escherichia coli cells expressing the tpm gene encoding the bacterial thiopurine methyltransferase (bTPMT) are shown to methylate selenite and (methyl)selenocysteine into dimethylselenide (DMSe) and dimethyldiselenide (DMDSe). E. coli cells expressing tpm from a gene library cosmid clone (harboring a Pseudomonas syrin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.11.3146-3149.2002

    authors: Ranjard L,Prigent-Combaret C,Nazaret S,Cournoyer B

    更新日期:2002-06-01 00:00:00