Abstract:
:A group of proteic toxin-antitoxin (TA) cassettes whose representatives are widely distributed among bacterial genomes has been identified. These cassettes occur in chromosomes, plasmids, bacteriophages, and noncomposite transposons, as well as in the SXT conjugative element of Vibrio cholerae. The following four homologous loci were subjected to detailed comparative studies: (i) tad-ata from plasmid pAMI2 of Paracoccus aminophilus (the prototype of this group), (ii) gp49-gp48 from the linear bacteriophage N15 of Escherichia coli, (iii) s045-s044 from SXT, and (iv) Z3230-Z3231 from the genomic island of enterohemorrhagic Escherichia coli O157:H7 strain EDL933. Functional analysis revealed that all but one of these loci (Z3230-Z3231) are able to stabilize heterologous replicons, although the host ranges varied. The TA cassettes analyzed have the following common features: (i) the toxins are encoded by the first gene of each operon; (ii) the antitoxins contain a predicted helix-turn-helix motif of the XRE family; and (iii) the cassettes have two promoters that are different strengths, one which is located upstream of the toxin gene and one which is located upstream of the antitoxin gene. All four toxins tested are functional in E. coli; overexpression of the toxins (in the absence of antitoxin) results in a bacteriostatic effect manifested by elongation of bacterial cells and growth arrest. The toxins have various effects on cell viability, which suggests that they may recognize different intracellular targets. Preliminary data suggest that different cellular proteases are involved in degradation of antitoxins encoded by the loci analyzed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Dziewit L,Jazurek M,Drewniak L,Baj J,Bartosik Ddoi
10.1128/JB.01610-06subject
Has Abstractpub_date
2007-03-01 00:00:00pages
1983-97issue
5eissn
0021-9193issn
1098-5530pii
JB.01610-06journal_volume
189pub_type
杂志文章abstract::Inositol derivative compounds provide a nutrient source for soil bacteria that possess the ability to degrade such compounds. Rhizobium strains that are capable of utilizing certain inositol derivatives are better colonizers of their host plants. We have cloned and determined the nucleotide sequence of the myo-inosito...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.8.2595-2604.2001
更新日期:2001-04-01 00:00:00
abstract::Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535-545. 1966.-Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.535-545.1966
更新日期:1966-02-01 00:00:00
abstract::Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1306-1312.1962
更新日期:1962-06-01 00:00:00
abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4288-4296.1991
更新日期:1991-07-01 00:00:00
abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05245-11
更新日期:2011-08-01 00:00:00
abstract::This study describes a novel approach to identify unique genomic DNA sequences from the unsequenced strain C. jejuni ATCC 43431 by comparison with the sequenced strain C. jejuni NCTC 11168. A shotgun DNA microarray was constructed by arraying 9,600 individual DNA fragments from a C. jejuni ATCC 43431 genomic library o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.14.4781-4795.2004
更新日期:2004-07-01 00:00:00
abstract::"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00137-06
更新日期:2006-06-01 00:00:00
abstract::The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.327-330.1992
更新日期:1992-01-01 00:00:00
abstract::Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Isolation and preliminary characterization of bacteriophage phimu-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage phimu-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.999-1002.1964
更新日期:1964-05-01 00:00:00
abstract::Shah, D. B. (University of Wisconsin, Madison), and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. II. Characterization of the lipase activity. J. Bacteriol. 89:949-953. 1965.-The staphylococcal egg yolk factor was characterized as a lipase. The enzyme had an optimal pH of 7.8, but the optimal pH of stability...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.949-953.1965
更新日期:1965-04-01 00:00:00
abstract::Gene amplifications have been detected as a transitory phenomenon in bacterial cultures. They are predicted to contribute to rapid adaptation by simultaneously increasing the expression of genes clustered on the chromosome. However, genome amplifications have rarely been described in natural isolates. Through DNA arra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01357-07
更新日期:2008-01-01 00:00:00
abstract::Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2540-2550.2005
更新日期:2005-04-01 00:00:00
abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.345-356.1967
更新日期:1967-01-01 00:00:00
abstract::Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00233-11
更新日期:2011-09-01 00:00:00
abstract::Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.370-380.1962
更新日期:1962-02-01 00:00:00
abstract::Cores are large, rod-shaped structures that have been found almost exclusively in group D streptococci, measure 0.1 to 0.16 mum in diameter, and extend the width or length of cells. This study has shown that cores are produced in the cells at a reproducible point in early stationary growth after extensive mesosomal fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.445-456.1977
更新日期:1977-01-01 00:00:00
abstract::We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.457-462.1991
更新日期:1991-01-01 00:00:00
abstract::Bacteroides ovatus, a human colonic anaerobe, utilizes the galactomannan guar gum as a sole source of carbohydrate. Previously, we found that none of the galactomannan-degrading enzymes were extracellular, and we characterized an outer membrane mannanase which hydrolyzes the backbone of guar gum to produce large fragm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2038-2043.1987
更新日期:1987-05-01 00:00:00
abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.19.5508-5512.2002
更新日期:2002-10-01 00:00:00
abstract::Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00075-12
更新日期:2012-04-01 00:00:00
abstract::Xylella fastidiosa possesses both type I and type IV pili at the same cell pole. By use of a microfluidic device, the speed of twitching movement by wild-type cells on a glass surface against the flow direction of media was measured as 0.86 (standard error [SE], 0.04) microm min(-1). A type I pilus mutant (fimA) moved...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00934-07
更新日期:2007-10-01 00:00:00
abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4417-4425.1987
更新日期:1987-10-01 00:00:00
abstract::Amplification of the mraY gene, previously called open reading frame Y (ORF-Y, 1,080 bp), at 2 min in the chromosome map of Escherichia coli enhanced the activity of UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase (EC 2.7.8.13). This enzyme catalyzes the form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1021-1026.1991
更新日期:1991-02-01 00:00:00
abstract:UNLABELLED:Many bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of productio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02596-14
更新日期:2015-07-01 00:00:00
abstract::The seven proteins encoded by the comG operon of Bacillus subtilis exhibit similarity to gene products required for the assembly of type 4 pili and for the secretion of certain proteins in gram-negative bacteria. Although polar transposon insertions in comG result in the loss of transformability and in the failure of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.1.41-45.1998
更新日期:1998-01-01 00:00:00
abstract::Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.6.1786-1792.1999
更新日期:1999-03-01 00:00:00
abstract::A protein homooligomer was purified from both the cell envelope fractions and the saline extracts of Rhodopseudomonas sphaeroides cells. This oligomer exhibited strong porin activity when reconstituted into proteoliposomes with egg phosphatidylcholine. In the saline extracts of both chemotrophically and phototrophical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.199-205.1984
更新日期:1984-07-01 00:00:00
abstract::A proline analogue, 4,5-dehydro-l-pipecolic acid (baikiain) induces the formation in Salmonella typhimurium of the two enzymes catalyzing the degradation of proline, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid (P5C) dehydrogenase. The level of induction by 20 mm baikiain is about 10% of the maximum level ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.3.1134-1141.1972
更新日期:1972-12-01 00:00:00
abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.231-238.1968
更新日期:1968-01-01 00:00:00
abstract::Escherichia coli cells expressing the tpm gene encoding the bacterial thiopurine methyltransferase (bTPMT) are shown to methylate selenite and (methyl)selenocysteine into dimethylselenide (DMSe) and dimethyldiselenide (DMDSe). E. coli cells expressing tpm from a gene library cosmid clone (harboring a Pseudomonas syrin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.11.3146-3149.2002
更新日期:2002-06-01 00:00:00