High-frequency RecA-dependent and -independent mechanisms of Congo red binding mutations in Yersinia pestis.

abstract：:Many streptococcal pathogens require a polysaccharide capsule for survival in the host during systemic infection. The highly conserved CpsA protein is proposed to be a transcriptional regulator of capsule production in streptococci, although the regulatory mechanism is unknown. Hydropathy plots of CpsA predict an inte...

journal_title：Journal of bacteriology

authors： Hanson BR,Lowe BA,Neely MN

更新日期：2011-01-01 00:00:00

abstract：:During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

journal_title：Journal of bacteriology

authors： Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

更新日期：1990-02-01 00:00:00

abstract：:We have presented experimental evidence to support the model that gliding motility of Myxococcus xanthus is driven by surface tension. (i) Motility is inhibited by the addition of sufficient exogenous, nontoxic surfactants to swamp out the cells' own surfactant gradient. (ii) M. xanthus does not move polystyrene latex...

journal_title：Journal of bacteriology

authors： Dworkin M,Keller KH,Weisberg D

更新日期：1983-09-01 00:00:00

abstract：:The activation of the Pm promoter of the meta operon of the TOL plasmid of Pseudomonas putida by its cognate XylS activator protein in the presence and absence of benzoate inducers has been examined in specialized Escherichia coli strains carrying Pm-lacZ fusions and the xylS gene in different configurations in which ...

journal_title：Journal of bacteriology

authors： Kessler B,Herrero M,Timmis KN,de Lorenzo V

更新日期：1994-06-01 00:00:00

abstract：:The spoVM gene encodes a 26-amino-acid polypeptide that is essential for spore formation in Bacillus subtilis. A transposon insertion within the spoVM open reading frame has been shown to encode a chimeric protein which is biologically inactive and produces a phenotype identical to that of a deletion and insertion mut...

journal_title：Journal of bacteriology

authors： Cutting S,Anderson M,Lysenko E,Page A,Tomoyasu T,Tatematsu K,Tatsuta T,Kroos L,Ogura T

更新日期：1997-09-01 00:00:00

abstract：:Colicinogenic factors ColI and ColV, which have been shown to behave as sex factors, could not be induced with mitomycin C. In contrast, the ColE(1), ColE(2), and ColE(3) factors, which do not exhibit any fertility factor characteristics, are inducible by this agent. The induced production of colicins E(1), E(2), and ...

journal_title：Journal of bacteriology

authors： Herschman HR,Helinski DR

更新日期：1967-09-01 00:00:00

abstract：:Many bacteria can accumulate glycine betaine for osmoprotection and catabolize it as a growth substrate, but how they regulate these opposing roles is poorly understood. In Pseudomonas syringae B728a, expression of the betaine catabolism genes was reduced by an osmotic upshift to an intermediate stress level, consiste...

journal_title：Journal of bacteriology

authors： Li S,Yu X,Beattie GA

更新日期：2013-05-01 00:00:00

abstract：:The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...

journal_title：Journal of bacteriology

authors： Lohrke SM,Nechaev S,Yang H,Severinov K,Jin SJ

更新日期：1999-08-01 00:00:00

abstract：:The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

journal_title：Journal of bacteriology

authors： Pollock JJ,Linder R,Salton MR

更新日期：1971-07-01 00:00:00

abstract：:Penicillin-binding proteins of 180, 89, 80, 68, 61, 41, and 38 kilodaltons were identified in Treponema pallidum (Nichols) by their covalent binding of [35S]benzylpenicillin. Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plas...

journal_title：Journal of bacteriology

authors： Cunningham TM,Miller JN,Lovett MA

更新日期：1987-11-01 00:00:00

abstract：:Swarmer cell differentiation is a complex process involving the activity of many gene products. In this report, we characterized the genetic locus of Tn5 insertion in each of 12 mutants defective in swarmer cell elongation. The mutations fell into four categories affecting either flagellar biosynthesis or energetics, ...

journal_title：Journal of bacteriology

authors： Belas R,Goldman M,Ashliman K

更新日期：1995-02-01 00:00:00

abstract：:The E. coli RelA protein is a ribosome-dependent (p)ppGpp synthetase that is activated in response to amino acid starvation. RelA can be dissected both functionally and physically into two domains: The N-terminal domain (NTD) (amino acids [aa] 1 to 455) contains the catalytic domain of RelA, and the C-terminal domain ...

journal_title：Journal of bacteriology

authors： Gropp M,Strausz Y,Gross M,Glaser G

更新日期：2001-01-01 00:00:00

abstract：:Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4...

journal_title：Journal of bacteriology

authors： Willis SH,Kazmierczak KM,Carter RH,Rothman-Denes LB

更新日期：2002-09-01 00:00:00

abstract：:Starvation and cell density regulate the developmental expression of Myxococcus xanthus gene 4521. Three classes of mutants allow expression of this developmental gene during growth on nutrient agar, such that colonies of strains containing a Tn5 lac Omega4521 fusion are Lac(+). One class of these mutants inactivates ...

journal_title：Journal of bacteriology

authors： Guo D,Wu Y,Kaplan HB

更新日期：2000-08-01 00:00:00

abstract：:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

journal_title：Journal of bacteriology

authors： Hartmann RK,Toschka HY,Erdmann VA

更新日期：1991-04-01 00:00:00

abstract：:GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated an open reading frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 2...

journal_title：Journal of bacteriology

authors： Richter G,Ritz H,Katzenmeier G,Volk R,Kohnle A,Lottspeich F,Allendorf D,Bacher A

更新日期：1993-07-01 00:00:00

abstract：:Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved...

journal_title：Journal of bacteriology

authors： Nelson SS,Glocka PP,Agarwal S,Grimm DP,McBride MJ

更新日期：2007-10-01 00:00:00

abstract：:In Escherichia coli, putrescine is metabolized to succinate for use as a carbon and nitrogen source by the putrescine utilization pathway (Puu pathway). One gene in the puu gene cluster encodes a transcription factor, PuuR, which has a helix-turn-helix DNA-binding motif. DNA microarray analysis of an E. coli puuR muta...

journal_title：Journal of bacteriology

authors： Nemoto N,Kurihara S,Kitahara Y,Asada K,Kato K,Suzuki H

更新日期：2012-07-01 00:00:00

abstract：:The three-component naphthalene dioxygenase (NDO) enzyme system carries out the first step in the aerobic degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The three-dimensional structure of NDO revealed that several of the amino acids at the active site of the oxygenase are hydrophobic, which is consi...

journal_title：Journal of bacteriology

authors： Parales RE,Lee K,Resnick SM,Jiang H,Lessner DJ,Gibson DT

更新日期：2000-03-01 00:00:00

abstract：:The intracellular levels of cyclic adenosine 3',5'-monophosphate (cyclic AMP) were measured at various intervals during growth and morphogenesis in Arthrobacter crystallopoietes. Cyclic AMP levels remained relatively constant throughout growth in spherical cells grown in glucose-based media. Immediately after inoculat...

journal_title：Journal of bacteriology

authors： Hamilton RW,Achberger EC,Kolenbrander PE

更新日期：1977-02-01 00:00:00

abstract：:Heterogeneity in colony size of the murine pathogen Mycoplasma pulmonis was examined. Subcloning experiments showed that colony size variation resulted from high-frequency genetic changes. About 3% of the colonies from any given subclone were variants, with as much as a fourfold change in colony diameter. When the var...

journal_title：Journal of bacteriology

authors： Dybvig K,Simecka JW,Watson HL,Cassell GH

更新日期：1989-09-01 00:00:00

abstract：:Shigella flexneri is a gram-negative bacterium that causes diarrhea and dysentery by invasion and spread through the colonic epithelium. Bacteria spread by assembling actin and other cytoskeletal proteins of the host into "actin tails" at the bacterial pole; actin tail assembly provides the force required to move bact...

journal_title：Journal of bacteriology

authors： Charles M,Magdalena J,Theriot JA,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its ex...

journal_title：Journal of bacteriology

authors： Suh Y,Benedik MJ

更新日期：1997-02-01 00:00:00

abstract：:In Escherichia coli, the frequencies of IS50 and Tn5 transposition are greater in Dam- cells than in isogenic Dam+ cells. IS50 transposition is increased approximately 1,000-fold and Tn5 transposition frequencies are increased about 5- to 10-fold in the absence of Dam methylation. However, in cells that are deficient ...

journal_title：Journal of bacteriology

authors： Makris JC,Nordmann PL,Reznikoff WS

更新日期：1990-03-01 00:00:00

abstract：:Aerobactin, a dihydroxamate siderophore produced by many strains of enteric bacteria, stimulated the growth of Neisseria gonorrhoeae FA19 and F62 in iron-limiting medium. However, gonococci did not produce detectable amounts of aerobactin in the Escherichia coli LG1522 aerobactin bioassay. We probed gonococcal genomic...

journal_title：Journal of bacteriology

authors： West SE,Sparling PF

更新日期：1987-08-01 00:00:00

abstract：:The generalized transducing phage T4GT7 grew well on recA strains of Escherichia coli and transduced recA into F- and Hfr strains of E. coli at high frequency. ...

journal_title：Journal of bacteriology

authors： Plakidou S,Moffat KG,Salmond GP,Mackinnon G

更新日期：1984-09-01 00:00:00

abstract：:Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....

journal_title：Journal of bacteriology

authors： Vogel AM,Das A

更新日期：1992-01-01 00:00:00

abstract：:The specificity of the growth requirement of Methanobacterium ruminantium strain M1 for a new coenzyme, 2-mercaptoethanesulfonic acid (HS--CoM), was examined. A variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme M were tested; only a restricted range of thioether, thioester, and thiocar...

journal_title：Journal of bacteriology

authors： Balch WE,Wolfe RS

更新日期：1979-01-01 00:00:00

abstract：:Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...

journal_title：Journal of bacteriology

authors： Smith RV,Evans MC

更新日期：1971-03-01 00:00:00

abstract：:Bacteria commonly live in dense polymicrobial communities and compete for scarce resources. Consequently, they employ a diverse array of mechanisms to harm, inhibit and kill their competitors. The cell wall is essential for bacterial survival by providing mechanical strength to resist osmotic stress. Because peptidogl...

journal_title：Journal of bacteriology

authors： Sibinelli-Sousa S,Hespanhol JT,Bayer-Santos E

更新日期：2020-11-02 00:00:00