Convenient transduction of recA with bacteriophage T4GT7.

abstract：:The virulence of Staphylococcus aureus is controlled by the accessory gene regulator (agr) system, including an extracellular inducer encoded by agrD. Variable agr PCR restriction fragment length polymorphism (RFLP) patterns of unique S. aureus strains (n = 192) were determined for a region comprising agrD and parts o...

journal_title：Journal of bacteriology

authors： van Leeuwen W,van Nieuwenhuizen W,Gijzen C,Verbrugh H,van Belkum A

更新日期：2000-10-01 00:00:00

abstract：:A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...

journal_title：Journal of bacteriology

authors： Day JM,Janssen GR

更新日期：2004-10-01 00:00:00

abstract：:The heat-stable enterotoxin (ST) produced by enterotoxigenic Escherichia coli is an extracellular peptide toxin that evokes watery diarrhea in the host. Two types of STs, STI and STII, have been found. Both STs are synthesized as precursor proteins and are then converted to the active forms with intramolecular disulfi...

journal_title：Journal of bacteriology

authors： Yamanaka H,Kobayashi H,Takahashi E,Okamoto K

更新日期：2008-12-01 00:00:00

abstract：:The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...

journal_title：Journal of bacteriology

authors： Maul RW,Sanders LH,Lim JB,Benitez R,Sutton MD

更新日期：2007-07-01 00:00:00

abstract：:Expression of the Bacillus licheniformis alpha-amylase gene, amyL, was temporally activated and subject to catabolite repression both in its natural host and when cloned on a 3.55-kilobase fragment in Bacillus subtilis. A subclone from which the promoter region of amyL and sequences upstream from the promoter were del...

journal_title：Journal of bacteriology

authors： Laoide BM,Chambliss GH,McConnell DJ

更新日期：1989-05-01 00:00:00

abstract：:The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (p...

journal_title：Journal of bacteriology

authors： Hall RM,Brown HJ,Brookes DE,Stokes HW

更新日期：1994-10-01 00:00:00

abstract：:Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640. 1964.-The cultural conditions for toxin production by Clostridium novyi were investigated, and the optimal constituents of a proper medium were determined. A...

journal_title：Journal of bacteriology

authors： NISHIDA S,NAKAGAWARA G

更新日期：1964-12-01 00:00:00

abstract：:This is the first study of sporogenesis in Streptomyces carried out on a relatively high number of species (seven) which allows us, using also previously published results, to establish a general picture of this process. In the sporogenesis of Streptomyces two basic stages can be considered: the sporulation septum syn...

journal_title：Journal of bacteriology

authors： Hardisson C,Manzanal MB

更新日期：1976-09-01 00:00:00

abstract：:A pattern of allosteric control for aromatic biosynthesis in cyanobacteria relies upon early-pathway regulation as the major control point for the entire branched pathway. In Synechococcus sp. strain PCC6301 (Anacystis nidulans), two enzymes which form precursors for L-phenylalanine biosynthesis are subject to control...

journal_title：Journal of bacteriology

authors： Hall GC,Flick MB,Jensen RA

更新日期：1983-01-01 00:00:00

abstract：:We investigated the effect of time, temperature, and the presence of sodium chloride, nitrates, and nitrites in the medium on the growth and production of enterotoxin B by Staphylococcus aureus. Assays by the double gel-diffusion method showed that maximal enterotoxin B production occurs at the beginning of the statio...

journal_title：Journal of bacteriology

authors： McLean RA,Lilly HD,Alford JA

更新日期：1968-04-01 00:00:00

abstract：:Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...

journal_title：Journal of bacteriology

authors： Camakaris J,Pittard J

更新日期：1971-08-01 00:00:00

abstract：:Well-characterized Salmonella mutants formerly used in biosynthetic studies of lipopolysaccharides were used to study the toxic portion of the complex endotoxin. Endotoxins prepared from wild types and their mutants were tested for their biological activities, including pyrogenicity, lethality, and immunogenicity. The...

journal_title：Journal of bacteriology

authors： Kim YB,Watson DW

更新日期：1967-11-01 00:00:00

abstract：:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...

journal_title：Journal of bacteriology

authors： Eisenberg RC,Dobrogosz WJ

更新日期：1967-03-01 00:00:00

abstract：:Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacer...

journal_title：Journal of bacteriology

authors： de Cristóbal RE,Vincent PA,Salomón RA

更新日期：2008-02-01 00:00:00

abstract：:DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a po...

journal_title：Journal of bacteriology

authors： Maupin-Furlow JA,Rosentel JK,Lee JH,Deppenmeier U,Gunsalus RP,Shanmugam KT

更新日期：1995-09-01 00:00:00

abstract：:Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...

journal_title：Journal of bacteriology

authors： Moen MN,Knævelsrud I,Haugland GT,Grøsvik K,Birkeland NK,Klungland A,Bjelland S

更新日期：2011-09-01 00:00:00

abstract：:The transcriptional control of the kdpFABC (K+ transport) operon of Salmonella typhimurium was characterized with a lacZ fusion. The kdpFABC operon of this organism was induced by K+ limitation and high osmolality, and osmotic induction was antagonized by a high concentration of K+. In the trkA (sapG) kdp+ mutant back...

journal_title：Journal of bacteriology

authors： Frymier JS,Reed TD,Fletcher SA,Csonka LN

更新日期：1997-05-01 00:00:00

abstract：:It previously has been proposed that the Escherichia coli SecB protein promotes the export of the maltose-binding protein (MBP) from the cytoplasm by preventing the folding of the precursor MBP (preMBP) into a translocation-incompetent conformation. The export of wild-type MBP is only partially blocked in SecB- cells....

journal_title：Journal of bacteriology

authors： Collier DN,Bassford PJ Jr

更新日期：1989-09-01 00:00:00

abstract：:In Escherichia coli, the following genes are involved in motility and chemotaxis. The H gene is the structural gene for flagellin. Mutation in the mot gene results in paralysis of the flagella, and mutation in the fla genes leads to an absence of flagella. The cheA, cheB, and cheC genes are required for chemotaxis. Th...

journal_title：Journal of bacteriology

authors： Armstrong JB,Adler J

更新日期：1969-01-01 00:00:00

abstract：:Strains of Staphylococcus aureus, an important human pathogen, display up to 20% variability in their genome sequence, and most sequence information is available for human clinical isolates that have not been subjected to genetic analysis of virulence attributes. S. aureus strain Newman, which was also isolated from a...

journal_title：Journal of bacteriology

authors： Baba T,Bae T,Schneewind O,Takeuchi F,Hiramatsu K

更新日期：2008-01-01 00:00:00

abstract：:We isolated an unusual mutant in which the arabinose operon carried an araC lesion that led to a dominant fucose resistance while retaining recessive constitutivity. All previously characterized C(c) mutations have been recessive for both fucose resistance and constitutivity. ...

journal_title：Journal of bacteriology

authors： Nathanson NM,Schleif R

更新日期：1973-08-01 00:00:00

abstract：:The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the prod...

journal_title：Journal of bacteriology

authors： Brinsmade SR,Escalante-Semerena JC

更新日期：2004-03-01 00:00:00

abstract：:Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of th...

journal_title：Journal of bacteriology

authors： Molenaar D,Hagting A,Alkema H,Driessen AJ,Konings WN

更新日期：1993-09-01 00:00:00

abstract：:soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...

journal_title：Journal of bacteriology

authors： Wu J,Weiss B

更新日期：1991-05-01 00:00:00

abstract：:The effect of heat shock on Myxococcus xanthus was investigated during both glycerol- and starvation-induced development. Cells heat shocked at 40 degrees C for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees C. Additionally, M. xanthus cells heat shocked p...

journal_title：Journal of bacteriology

authors： Killeen KP,Nelson DR

更新日期：1988-11-01 00:00:00

abstract：:The spoIIG promoter is used by RNA polymerase containing sigma A (E sigma A), the primary form of RNA polymerase found in vegetative cells in Bacillus subtilis. However, the spoIIG promoter is active only after the onset of sporulation. Activation of the spoIIG promoter requires the product of the spo0A gene (Spo0A). ...

journal_title：Journal of bacteriology

authors： Satola SW,Baldus JM,Moran CP Jr

更新日期：1992-03-01 00:00:00

abstract：:Isolated membrane vesicles from the obligately acidophilic bacterium Bacillus acidocaldarius generated an electrochemical gradient of protons (delta mu- H+) upon energization with ascorbate-phenazine methosulfate at pH 6.0 or 3.0. At pH 6.0, there was little or no transmembrane pH gradient (delta pH), but a transmembr...

journal_title：Journal of bacteriology

authors： Guffanti AA,Mann M,Sherman TL,Krulwich TA

更新日期：1984-08-01 00:00:00

abstract：:The location of the Escherichia coli K-12 genes determining or regulating glutamate transport, and the location of the gene determining glutamate decarboxylase synthesis, were established by conjugation. The ability to grow on glutamate as the sole source of carbon and energy was used to select for glutamate transport...

journal_title：Journal of bacteriology

authors： Marcus M,Halpern YS

更新日期：1967-04-01 00:00:00

abstract：:The bacterium Rhodobacter capsulatus is capable of an unusual form of genetic exchange, mediated by a transducing bacteriophage-like particle called the gene transfer agent (GTA). GTA production by R. capsulatus is controlled at the level of transcription by a cellular two-component signal transduction system that inc...

journal_title：Journal of bacteriology

authors： Lang AS,Beatty JT

更新日期：2002-02-01 00:00:00

abstract：:An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the...

journal_title：Journal of bacteriology

authors： Kong D,Masker W

更新日期：1994-10-01 00:00:00