Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli.

Abstract:

:soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requires both soxR and a new gene, soxS. soxS is adjacent to soxR, it encodes a 13-kDa protein, and it is required for paraquat resistance. These functions were revealed by studies in which the sequence of the 1.1-kb soxR-soxS region was determined, the 5' ends of the mRNAs were mapped, and complementation tests were performed with soxRS plasmids containing deletions of known sequence. The two genes are divergently transcribed, and the transcripts overlap. The soxS promoter is within the 85-nucleotide intergenic region, whereas the soxR promoter is within soxS. soxS mRNA increases after induction. Both protein products have possible DNA-binding (helix-turn-helix) domains. SoxR contains four cysteines (CX2CXCX5C) that might be part of a sensor region. SoxS shows 17 to 31% homology to the C-terminal portions of members of the AraC family of positive regulators.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wu J,Weiss B

doi

10.1128/jb.173.9.2864-2871.1991

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

2864-71

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

杂志文章
  • Lysine catabolism in Streptomyces spp. is primarily through cadaverine: beta-lactam producers also make alpha-aminoadipate.

    abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.1.299-302.1989

    authors: Madduri K,Stuttard C,Vining LC

    更新日期:1989-01-01 00:00:00

  • Role of a ferredoxin gene cotranscribed with the nifHDK operon in N(2) fixation and nitrogenase "switch-off" of Azoarcus sp. strain BH72.

    abstract::The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.12.3752-3760.2001

    authors: Egener T,Martin DE,Sarkar A,Reinhold-Hurek B

    更新日期:2001-06-01 00:00:00

  • Purification and preliminary characterization of the Escherichia coli K-12 recF protein.

    abstract::The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene produ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.11.6291-6299.1990

    authors: Griffin TJ 4th,Kolodner RD

    更新日期:1990-11-01 00:00:00

  • DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli.

    abstract::The GcvA protein is a LysR family regulatory protein necessary for both activation and repression of the Escherichia coli glycine cleavage enzyme operon (gcv) and negative regulation of gcvA. Gel shift assays indicated that overexpressed GcvA in crude extracts is capable of binding specifically to DNA containing the g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.17.4940-4946.1995

    authors: Wilson RL,Urbanowski ML,Stauffer GV

    更新日期:1995-09-01 00:00:00

  • Role for free isoleucine of glycyl-leucine in the repression of threonine deaminase in Escherichia coli.

    abstract::In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.1.29-39.1974

    authors: Wasmuth JJ,Umbarger HE

    更新日期:1974-01-01 00:00:00

  • Isolation and characterization of the cis-trans-unsaturated fatty acid isomerase of Pseudomonas oleovorans GPo12.

    abstract::Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3256-3261.1999

    authors: Pedrotta V,Witholt B

    更新日期:1999-05-01 00:00:00

  • ISOLOGOUS INTERFERENCE WITH ULTRAVIOLET AND X-RAY IRRADIATED BACTERIOPHAGE T2.

    abstract::Levy, Stuart B. (Institut du Radium, Paris, France). Isologous interference with ultraviolet and X-ray irradiated bacteriophage T(2). J. Bacteriol. 87:1330-1338. 1964.-Qualitative and quantitative analysis of the interference capacity of an irradiated T(2) bacteriophage was made with ultraviolet and X-ray irradiation....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.6.1330-1338.1964

    authors: LEVY SB

    更新日期:1964-06-01 00:00:00

  • Nature of the promoter activated by C.PvuII, an unusual regulatory protein conserved among restriction-modification systems.

    abstract::A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.2.488-497.2005

    authors: Knowle D,Lintner RE,Touma YM,Blumenthal RM

    更新日期:2005-01-01 00:00:00

  • Discovery of a nonclassical siderophore, legiobactin, produced by strains of Legionella pneumophila.

    abstract::The mechanisms by which Legionella pneumophila, a facultative intracellular parasite and the agent of Legionnaires' disease, acquires iron are largely unexplained. Several earlier studies indicated that L. pneumophila does not elaborate siderophores. However, we now present evidence that supernatants from L. pneumophi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.3.749-757.2000

    authors: Liles MR,Scheel TA,Cianciotto NP

    更新日期:2000-02-01 00:00:00

  • Aquaporin Z of Escherichia coli: reassessment of its regulation and physiological role.

    abstract::Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased transcription in enriched medium required the RpoS sigma factor. Expression of the aqpZ-lac fusion was not affected by up- o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.15.4304-4307.2002

    authors: Soupene E,King N,Lee H,Kustu S

    更新日期:2002-08-01 00:00:00

  • Halocin S8: a 36-amino-acid microhalocin from the haloarchaeal strain S8a.

    abstract::Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.17.4951-4958.2000

    authors: Price LB,Shand RF

    更新日期:2000-09-01 00:00:00

  • Temperature Control of psaA Expression by PsaE and PsaF in Yersinia pestis.

    abstract::PsaA, the subunit of the fimbria originally referred to as the "pH 6 antigen," is required for full virulence of Yersinia pestis during bubonic plague. The expression of psaA is dependent upon specific environmental signals, and while the signals (high temperature and acidic pH) are defined, the mechanisms underlying ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00217-19

    authors: Quinn JD,Weening EH,Miner TA,Miller VL

    更新日期:2019-07-24 00:00:00

  • Regulation of the Escherichia coli hfq gene encoding the host factor for phage Q beta.

    abstract::The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.2.531-534.1994

    authors: Kajitani M,Kato A,Wada A,Inokuchi Y,Ishihama A

    更新日期:1994-01-01 00:00:00

  • The Yersinia Yop virulon: LcrV is required for extrusion of the translocators YopB and YopD.

    abstract::LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.5.1207-1214.1998

    authors: Sarker MR,Neyt C,Stainier I,Cornelis GR

    更新日期:1998-03-01 00:00:00

  • Identification and complementation of frameshift mutations associated with loss of cytadherence in Mycoplasma pneumoniae.

    abstract::Mycoplasma pneumoniae cytadherence is mediated by a specialized, polar attachment organelle. Certain spontaneously arising cytadherence mutants (designated class I) lack HMW2, fail to localize the adhesin protein P1 to the attachment organelle, and exhibit accelerated turnover of proteins HMW1, HMW3, and P65. Insertio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.14.4404-4410.1999

    authors: Fisseha M,Göhlmann HW,Herrmann R,Krause DC

    更新日期:1999-07-01 00:00:00

  • Covalent association of beta-1,3-glucan with beta-1,6-glucosylated mannoproteins in cell walls of Candida albicans.

    abstract::Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a beta-1,3-glucan antiserum. They lost their beta-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, sugges...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.13.3788-3792.1995

    authors: Kapteyn JC,Montijn RC,Dijkgraaf GJ,Van den Ende H,Klis FM

    更新日期:1995-07-01 00:00:00

  • Immunological response of three mouse strains to typhoid vaccine and Vi antigen.

    abstract::Vi-agglutinin, active cutaneous anaphylaxis and protective responses (ed(50)) of three mouse strains (CFW, NIH, and Balb/cAnN) to acetone-inactivated typhoid vaccine and soluble Vi antigen were compared. Seven days after immunization with either typhoid vaccine or Vi antigen the three strains of mice differed with res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.8-12.1969

    authors: Esposito VM,Feeley JC,Leeder WD,Pittman M

    更新日期:1969-07-01 00:00:00

  • Purification and characterization of PBP4a, a new low-molecular-weight penicillin-binding protein from Bacillus subtilis.

    abstract::Penicillin-binding protein 4a (PBP4a) from Bacillus subtilis was overproduced and purified to homogeneity. It clearly exhibits DD-carboxypeptidase and thiolesterase activities in vitro. Although highly isologous to the Actinomadura sp. strain R39 DD-peptidase (B. Granier, C. Duez, S. Lepage, S. Englebert, J. Dusart, O...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1595-1599.2001

    authors: Duez C,Vanhove M,Gallet X,Bouillenne F,Docquier J,Brans A,Frère J

    更新日期:2001-03-01 00:00:00

  • Procedure for isolating mutants defective in metabolite transport or utilization.

    abstract::Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.3.1216-1218.1975

    authors: Berg CM,Rossi JJ,Coleman JV,Conlon M

    更新日期:1975-03-01 00:00:00

  • METHICILLIN-INDUCED LYSOZYME-SENSITIVE FORMS OF STAPHYLOCOCCI.

    abstract::Aldrich, K. M. (The University of Kansas, Lawrence), and C. P. Sword. Methicillin-induced lysozyme-sensitive forms of staphylococci. J. Bacteriol. 87:690-695. 1964.-Staphylococcus aureus and S. epidermidis grown in the presence of sublethal amounts of methicillin were converted to enlarged spheres within 2 to 4 hr, as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.690-695.1964

    authors: ALDRICH KM,SWORD CP

    更新日期:1964-03-01 00:00:00

  • Degradation of 3-phenylbutyric acid by Pseudomonas sp.

    abstract::Pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-PB) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. During early exponential growth, a catechol substance identified as 3-(2,3-di...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Sariaslani FS,Sudmeier JL,Focht DD

    更新日期:1982-10-01 00:00:00

  • Bacterial catalysis of nitrosation: involvement of the nar operon of Escherichia coli.

    abstract::We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.359-364.1988

    authors: Ralt D,Wishnok JS,Fitts R,Tannenbaum SR

    更新日期:1988-01-01 00:00:00

  • Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.

    abstract::Exponentially growing populations of Bacillus subtilis contain two morphologically and functionally distinct cell types: motile individuals and nonmotile multicellular chains. Motility differentiation arises because RNA polymerase and the alternative sigma factor sigma(D) activate expression of flagellin in a subpopul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00521-09

    authors: Chen R,Guttenplan SB,Blair KM,Kearns DB

    更新日期:2009-09-01 00:00:00

  • Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation.

    abstract::Mouse-virulent Salmonella typhimurium strains SR-11 and ATCC 14028-1s express curli fibers, thin aggregative fibers, at ambient temperature on plates as judged by Western blot analysis and electron microscopy. Concomitantly with curli expression, cells develop a rough and dry colony morphology and bind the dye Congo r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.3.722-731.1998

    authors: Römling U,Bian Z,Hammar M,Sierralta WD,Normark S

    更新日期:1998-02-01 00:00:00

  • Regulation of Bacteriodes fragilis katB mRNA by oxidative stress and carbon limitation.

    abstract::Regulation of the katB catalase gene in the anaerobic bacterium Bacteroides fragilis was studied. Northern blot hybridization analyses revealed that katB was transcribed as an approximately 1.6-kb monocistronic mRNA. The levels of katB mRNA increased > 15-fold when anaerobic, mid-logarithmic-phase cultures were expose...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7033-7039.1997

    authors: Rocha ER,Smith CJ

    更新日期:1997-11-01 00:00:00

  • Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions.

    abstract::Transcriptional fusions to Salmonella typhimurium chromosomal genes were constructed by integration of a suicide fusion vector into the chromosome by homologous recombination with random cloned chromosomal fragments. We describe here a transductional method using the generalized transducing phage of S. typhimurium, P2...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.21.7086-7091.1993

    authors: Mahan MJ,Slauch JM,Mekalanos JJ

    更新日期:1993-11-01 00:00:00

  • Differential regulation of two oligogalacturonate outer membrane channels, KdgN and KdgM, of Dickeya dadantii (Erwinia chrysanthemi).

    abstract::The entry of oligogalacturonates into Dickeya dadantii occurs through the specific channel KdgM. The genome of the bacterium encodes a second member of this family of outer membrane proteins, KdgN. We showed that this protein is also involved in the uptake of oligogalacturonates. When KdgN was reconstituted in proteol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00218-07

    authors: Condemine G,Ghazi A

    更新日期:2007-08-01 00:00:00

  • Nucleic acid hybridization analysis of an integrated plasmid in Staphylococcus aureus.

    abstract::A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1424-1428.1975

    authors: Novick R,Zouzias D,Rush M

    更新日期:1975-12-01 00:00:00

  • Nature of the complementation products formed by a complementing mutant of neurospora crassa.

    abstract::The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.3.787-792.1968

    authors: Sundaram TK,Fincham JR

    更新日期:1968-03-01 00:00:00

  • Phylogenetic analysis and evolution of RNase P RNA in proteobacteria.

    abstract::The secondary structures of the eubacterial RNase P RNAs are being elucidated by a phylogenetic comparative approach. Sequences of genes encoding RNase P RNA from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. These sequences allow the refinement, to ne...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3855-3863.1991

    authors: Brown JW,Haas ES,James BD,Hunt DA,Liu JS,Pace NR

    更新日期:1991-06-01 00:00:00