Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions.

abstract：:Acyl-homoserine lactone (acyl-HSL) quorum-sensing signaling is common to many Proteobacteria. Acyl-HSLs are synthesized by the LuxI family of synthases, and the signal response is mediated by members of the LuxR family of transcriptional regulators. Burkholderia thailandensis is a member of a closely related cluster o...

journal_title：Journal of bacteriology

authors： Chandler JR,Duerkop BA,Hinz A,West TE,Herman JP,Churchill ME,Skerrett SJ,Greenberg EP

更新日期：2009-10-01 00:00:00

abstract：:Vibrio cholerae hemolysin (HlyA) is a 65-kDa water-soluble pore-forming toxin that causes lysis of eukaryotic cells by destroying selective permeability of the plasma membrane bilayer. The HlyA monomer self-assembles on the target cell surface to the more stable beta-barrel amphipathic heptamer, which inserts into the...

journal_title：Journal of bacteriology

authors： Dutta S,Mazumdar B,Banerjee KK,Ghosh AN

更新日期：2010-01-01 00:00:00

abstract：:The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...

journal_title：Journal of bacteriology

authors： Umbreit JN,Strominger JL

更新日期：1972-12-01 00:00:00

abstract：:The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site, the two gene products TnpS and TnpT and (ii) the 2.4-kb tnpT-res-tnpS region is 48 kb apart from the tnpA gene (M. Tsuda,...

journal_title：Journal of bacteriology

authors： Genka H,Nagata Y,Tsuda M

更新日期：2002-09-01 00:00:00

abstract：:Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. Th...

journal_title：Journal of bacteriology

authors： Paustian ML,May BJ,Cao D,Boley D,Kapur V

更新日期：2002-12-01 00:00:00

abstract：:In Klebsiella aerogenes, arylsulfatase synthesis was repressed by inorganic sulfate, sulfite, sulfide, thiosulfate, and cysteine, but not by methionine under normal growth conditions. We isolated cysteine-requiring mutants (Cys minus), and mutants (AtsS minus, AtsR minus) in which the regulation of arylsulfatase synth...

journal_title：Journal of bacteriology

authors： Adachi T,Murooka Y,Harada T

更新日期：1975-01-01 00:00:00

abstract：:A procedure for efficient transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts with plasmid DNA was developed. Transformation frequencies with S. fradiae protoplasts were strongly influenced by the temperatures for cell growth, protoplast formation, and protoplast regeneration. Transformatio...

journal_title：Journal of bacteriology

authors： Matsushima P,Baltz RH

更新日期：1985-07-01 00:00:00

abstract：:Prolyl-phenylalanine-specific serine protease (dentilisin) is a major extracellular protease produced by Treponema denticola. The gene, prtP, coding for the protease was recently cloned and sequenced (K. Ishihara, T. Miura, H. K. Kuramitsu, and K. Okuda, Infect. Immun. 64:5178-5186, 1996). In order to determine the ro...

journal_title：Journal of bacteriology

authors： Ishihara K,Kuramitsu HK,Miura T,Okuda K

更新日期：1998-08-01 00:00:00

abstract：:Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

journal_title：Journal of bacteriology

authors： Fitz-James PC

更新日期：1971-03-01 00:00:00

abstract：:The propanediol utilization (pdu) operon of Salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme B12-dependent propanediol dehydratase. A clone that expresses propanediol dehydratase activity was isolated from a Salmonella genomic library. DNA sequence analysis showe...

journal_title：Journal of bacteriology

authors： Bobik TA,Xu Y,Jeter RM,Otto KE,Roth JR

更新日期：1997-11-01 00:00:00

abstract：:Acclimation to high salt concentrations involves concerted changes in gene expression. For the majority of salt-regulated genes, the mechanism underlying the induction process is not known. The gene ggpS (sll1566), which encodes the glucosylglycerol-phosphate synthase responsible for the synthesis of the compatible so...

journal_title：Journal of bacteriology

authors： Klähn S,Höhne A,Simon E,Hagemann M

更新日期：2010-09-01 00:00:00

abstract：:In Rhodobacter sphaeroides, MreB, MreC, MreD, PBP2, and RodA are encoded at the same locus. The localizations of PBP2, MreB, and MreC, which have all been implicated in the synthesis of the peptidoglycan layer, were investigated under different growth conditions to gain insight into the relationships between these pro...

journal_title：Journal of bacteriology

authors： Slovak PM,Porter SL,Armitage JP

更新日期：2006-03-01 00:00:00

abstract：:One of the pleiotropic phenotypes of mutations affecting DNA gyrase activity in Salmonella typhimurium is the constitutive deattenuation of the histidine operon. In the present work, we isolated and characterized a suppressor mutation which restores his attenuation in the presence of a defective gyrase. Such a suppres...

journal_title：Journal of bacteriology

authors： Blanc-Potard AB,Bossi L

更新日期：1994-04-01 00:00:00

abstract：:In Azotobacter vinelandii, activation of nif gene expression by the transcriptional regulatory enhancer binding protein NIFA is controlled by the sensor protein NIFL in response to changes in levels of oxygen and fixed nitrogen in vivo. NIFL is a novel redox-sensing flavoprotein which is also responsive to adenosine n...

journal_title：Journal of bacteriology

authors： Money T,Jones T,Dixon R,Austin S

更新日期：1999-08-01 00:00:00

abstract：:Cyanobacteria are monophyletic organisms that perform oxygenic photosynthesis. While they exhibit great diversity, they have a common set of genes. However, the essentiality of them for viability has hampered the elucidation of their functions. One example of these genes is cyabrB1 (also known as calA in Anabaena sp. ...

journal_title：Journal of bacteriology

authors： Higo A,Nishiyama E,Nakamura K,Hihara Y,Ehira S

更新日期：2019-08-08 00:00:00

abstract：:Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. Spontaneous mutants which are deficient for virulence and extracellular polysaccharide (Eps) production accumulate in large numbers in stationary-phase cultures of this bacterium, a phenomenon which we have called stationary-phase v...

journal_title：Journal of bacteriology

authors： Rajeshwari R,Sonti RV

更新日期：2000-09-01 00:00:00

abstract：:A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....

journal_title：Journal of bacteriology

authors： Sonstein SA,Hammel JM,Bondi A

更新日期：1971-08-01 00:00:00

abstract：:MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We al...

journal_title：Journal of bacteriology

authors： Chubiz LM,Rao CV

更新日期：2010-09-01 00:00:00

abstract：:The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

journal_title：Journal of bacteriology

authors： Amikam D,Benziman M

更新日期：1989-12-01 00:00:00

abstract：:A system for high-level expression of heparinase I, heparinase II, heparinase III, chondroitinase AC, and chondroitinase B in Flavobacterium heparinum is described. hepA, along with its regulatory region, as well as hepB, hepC, cslA, and cslB, cloned downstream of the hepA regulatory region, was integrated in the chro...

journal_title：Journal of bacteriology

authors： Blain F,Tkalec AL,Shao Z,Poulin C,Pedneault M,Gu K,Eggimann B,Zimmermann J,Su H

更新日期：2002-06-01 00:00:00

abstract：:Anabaenopeptins (AP) are bioactive cyclic hexapeptides synthesized nonribosomally in cyanobacteria. APs are characterized by several conserved motifs, including the ureido bond, N-methylation in position 5, and d-Lys in position 2. All other positions of the AP molecule are variable, resulting in numerous structural v...

journal_title：Journal of bacteriology

authors： Christiansen G,Philmus B,Hemscheidt T,Kurmayer R

更新日期：2011-08-01 00:00:00

abstract：:Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...

journal_title：Journal of bacteriology

authors： Beeser AE,Cooper TG

更新日期：1999-09-01 00:00:00

abstract：:rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...

journal_title：Journal of bacteriology

authors： Schneider DA,Gourse RL

更新日期：2003-10-01 00:00:00

abstract：:Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

journal_title：Journal of bacteriology

authors： HARPER MK,CURRAN HR,PALLANSCH MJ

更新日期：1964-11-01 00:00:00

abstract：:In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...

journal_title：Journal of bacteriology

authors： Whitworth DE,Holmes AB,Irvine AG,Hodgson DA,Scanlan DJ

更新日期：2008-03-01 00:00:00

abstract：:The blp locus of a type 6A strain of Streptococcus pneumoniae encodes a two-peptide bacteriocin, pneumocin MN, which mediates intraspecies competition during mouse nasopharyngeal colonization. This locus is regulated by a quorum-sensing mechanism consisting of a dedicated two-component regulatory system and a peptide ...

journal_title：Journal of bacteriology

authors： Dawid S,Sebert ME,Weiser JN

更新日期：2009-03-01 00:00:00

abstract：:Sialidase activity varies widely among strains and tends to correlate with strain virulence in the avian pathogen Mycoplasma synoviae. To characterize the forms of selection acting on enzymes required for sialic acid scavenging and catabolism, the ratios of nonsynonymous (K(a)) to synonymous (K(s)) mutation frequency ...

journal_title：Journal of bacteriology

authors： May M,Brown DR

更新日期：2009-06-01 00:00:00

abstract：:Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless...

journal_title：Journal of bacteriology

authors： Sato H,Watanabe T,Higuchi K,Teruya K,Ohtake A,Murata Y,Saito H,Aizawa C,Danbara H,Maehara N

更新日期：2000-07-01 00:00:00

abstract：:The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...

journal_title：Journal of bacteriology

authors： Moris M,Braeken K,Schoeters E,Verreth C,Beullens S,Vanderleyden J,Michiels J

更新日期：2005-08-01 00:00:00

abstract：:Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...

journal_title：Journal of bacteriology

authors： Kelliher JL,Leder Macek AJ,Grudzinski KM,Radin JN,Kehl-Fie TE

更新日期：2020-10-22 00:00:00