Isolation and properties of the complex between the enhancer binding protein NIFA and the sensor NIFL.

Abstract:

:In Azotobacter vinelandii, activation of nif gene expression by the transcriptional regulatory enhancer binding protein NIFA is controlled by the sensor protein NIFL in response to changes in levels of oxygen and fixed nitrogen in vivo. NIFL is a novel redox-sensing flavoprotein which is also responsive to adenosine nucleotides in vitro. Inhibition of NIFA activity by NIFL requires stoichiometric amounts of the two proteins, implying that the mechanism of inhibition is by direct protein-protein interaction rather than by catalytic modification of the NIFA protein. The formation of the inhibitory complex between NIFL and NIFA may be regulated by the intracellular ATP/ADP ratio. We show that adenosine nucleotides promote complex formation between purified NIFA and NIFL in vitro, allowing isolation of the NIFL-NIFA complex. The complex can also be isolated from cell extracts containing coexpressed NIFL and NIFA in the presence of MgADP. Removal of the nucleotide causes dissociation of the complex. Experiments with truncated proteins demonstrate that the amino-terminal domain of NIFA and the C-terminal region of NIFL potentiate the ADP-dependent stimulation of NIFL-NIFA complex formation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Money T,Jones T,Dixon R,Austin S

doi

10.1128/JB.181.15.4461-4468.1999

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

4461-8

issue

15

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

杂志文章
  • Localization of acid phosphatase activity in the basidia of Coprinus micaceus.

    abstract::Gill lamellae from the young fruiting bodies of the basidiomycete Coprinus micaceus possess cytoplasmic particles with cytochemically demonstrable acid phosphatase activity; they are presumed to be lysosomes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.1.443-446.1973

    authors: Nehemiah JL

    更新日期:1973-07-01 00:00:00

  • Simple method for identification of plasmid-coded proteins.

    abstract::Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.692-693.1979

    authors: Sancar A,Hack AM,Rupp WD

    更新日期:1979-01-01 00:00:00

  • Lactic acid translocation: terminal step in glycolysis by Streptococcus faecalis.

    abstract::Streptococcus faecalis obtains metabolic energy chiefly from the conversion of glucose to lactic acid; the present experiments deal with the mechanism of lactic acid translocation across the cytoplasmic membrane. Efflux of [(14)C]lactate from preloaded cells was accelerated by raising the external pH, and also by the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.3.1141-1148.1974

    authors: Harold FM,Levin E

    更新日期:1974-03-01 00:00:00

  • Isolation and characterization of a new plasmid from a Flavobacterium sp. which carries the genes for degradation of 2,4-dichlorophenoxyacetate.

    abstract::A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade thes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.3897-3902.1988

    authors: Chaudhry GR,Huang GH

    更新日期:1988-09-01 00:00:00

  • Cloning and characterization of a gene (mspA) encoding the major sheath protein of Treponema maltophilum ATCC 51939(T).

    abstract::The major sheath protein-encoding gene (mspA) of the oral spirochete Treponema maltophilum ATCC 51939(T) was cloned by screening a genomic library with an anti-outer membrane fraction antibody. The mspA gene encodes a precursor protein of 575 amino acids with a predicted molecular mass of 62.3 kDa, including a signal ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.3.1025-1029.1999

    authors: Heuner K,Choi BK,Schade R,Moter A,Otto A,Göbel UB

    更新日期:1999-02-01 00:00:00

  • Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

    abstract::A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.3.1072-1074.1974

    authors: Lederberg EM,Cohen SN

    更新日期:1974-09-01 00:00:00

  • Characterization of the extracellular cellulase from a mesophilic clostridium (strain C7).

    abstract::An extracellular, 700,000-Mr multiprotein complex that catalyzed the hydrolysis of crystalline cellulose (Avicel) was isolated from cultures of Clostridium sp. strain C7, a mesophile from freshwater sediment. In addition to cellulose (Avicel, ball-milled filter paper), the multiprotein complex hydrolyzed carboxymethyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4231-4237.1990

    authors: Cavedon K,Leschine SB,Canale-Parola E

    更新日期:1990-08-01 00:00:00

  • Developmental regulation of a Rhizobium cell surface antigen during growth of pea root nodules.

    abstract::A monoclonal antibody, AFRC MAC 203, was used to examine the expression of a nodule-induced cell surface antigen associated with lipopolysaccharide in Rhizobium leguminosarum bv. viciae 3841. Silver-enhanced immunogold-labeled tissue sections revealed that, in very young tissues of pea root nodules, the nodule-induced...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4537-4542.1989

    authors: VandenBosch KA,Brewin NJ,Kannenberg EL

    更新日期:1989-09-01 00:00:00

  • Cloning of Escherichia coli and Pseudomonas aeruginosa phosphomannose isomerase genes and their expression in alginate-negative mutants of Pseudomonas aeruginosa.

    abstract::The phosphomannose isomerase (pmi) gene of Escherichia coli was cloned on a broad-host-range cosmid vector and expressed in Pseudomonas aeruginosa at a low level. Plasmid pAD3, which harbors the E. coli pmi gene, contains a 6.2-kilobase-pair HindIII fragment derived from the chromosome of E. coli. Subcloning produced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.249-257.1985

    authors: Darzins A,Nixon LL,Vanags RI,Chakrabarty AM

    更新日期:1985-01-01 00:00:00

  • Complete genome sequence of Listeria seeligeri, a nonpathogenic member of the genus Listeria.

    abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01415-09

    authors: Steinweg C,Kuenne CT,Billion A,Mraheil MA,Domann E,Ghai R,Barbuddhe SB,Kärst U,Goesmann A,Pühler A,Weisshaar B,Wehland J,Lampidis R,Kreft J,Goebel W,Chakraborty T,Hain T

    更新日期:2010-03-01 00:00:00

  • Cloning and characterization of two immunophilin-like genes, ilpA and fkpA, on a single 3.9-kilobase fragment of Aeromonas hydrophila genomic DNA.

    abstract::Antiserum to Aeromonas hydrophila A6 cell envelopes was shown in a previous study (C. Y. F. Wong, G. Mayrhofer, M. W. Heuzenroeder, H. M. Atkinson, D. M. Quinn, and R. L. P. Flower, FEMS Immunol. Med. Microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. In this study, colony blot ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.11.3397-3403.1997

    authors: Wong CY,Heuzenroeder MW,Quinn DM,Flower RL

    更新日期:1997-06-01 00:00:00

  • Culture of Campylobacter jejuni with sodium deoxycholate induces virulence gene expression.

    abstract::Campylobacter jejuni, a spiral-shaped gram-negative bacterium, is a leading bacterial cause of human food-borne illness. Acute disease is associated with C. jejuni invasion of the intestinal epithelium. Further, maximal host cell invasion requires the secretion of proteins termed Campylobacter invasion antigens (Cia)....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01736-07

    authors: Malik-Kale P,Parker CT,Konkel ME

    更新日期:2008-04-01 00:00:00

  • Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms.

    abstract::Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.2.527-534.1981

    authors: Kawamoto I,Oka T,Nara T

    更新日期:1981-05-01 00:00:00

  • Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, phi EC2-resistant mutants.

    abstract::Outer membrane alterations were characterized in spontaneous mutants of the Erwinia chrysanthemi 3937jRH, which were selected for resistance to bacteriophage phi EC2. All but one of the mutants analyzed were affected in their lipopolysaccharide (LPS) structure, lacking the entire heterogeneous region of apparent high ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4011-4017.1987

    authors: Schoonejans E,Expert D,Toussaint A

    更新日期:1987-09-01 00:00:00

  • Identification and disruption of lisRK, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and virulence in Listeria monocytogenes.

    abstract::lisRK encodes a two-component regulatory system in the food pathogen Listeria monocytogenes LO28. Following identification of the operon in an acid-tolerant Tn917 mutant, a deletion in the histidine kinase component was shown to result in a growth phase variation in acid tolerance, an ability to grow in high ethanol c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.21.6840-6843.1999

    authors: Cotter PD,Emerson N,Gahan CG,Hill C

    更新日期:1999-11-01 00:00:00

  • Hydroxyethylthiazole uptake in Escherichia coli: general properties and relationship between uptake and thiamine biosynthesis.

    abstract::Uptake of (35)S-hydroxyethylthiazole (4-methyl-5-hydroxyethylthiazole) by Escherichia coli intact cells was studied. Hydroxyethylthiazole was taken up in the presence and absence of glucose at the same rate. The uptake was almost proportional to a hydroxyethylthiazole concentration gradient up to 0.1 mM with no tenden...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1280-1286.1973

    authors: Yamasaki H,Sanemori H,Yamada K,Kawasaki T

    更新日期:1973-12-01 00:00:00

  • TcpH influences virulence gene expression in Vibrio cholerae by inhibiting degradation of the transcription activator TcpP.

    abstract::Expression of toxT, the transcription activator of cholera toxin and pilus production in Vibrio cholerae, is the consequence of a complex cascade of regulatory events that culminates in activation of the toxT promoter by TcpP and ToxR, two membrane-localized transcription factors. Both are encoded in operons with gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.24.8309-8316.2004

    authors: Beck NA,Krukonis ES,DiRita VJ

    更新日期:2004-12-01 00:00:00

  • Characterization of the periplasmic region of PomB, a Na+-driven flagellar stator protein in Vibrio alginolyticus.

    abstract::The stator proteins PomA and PomB form a complex that couples Na(+) influx to torque generation in the polar flagellar motor of Vibrio alginolyticus. This stator complex is anchored to an appropriate place around the rotor through a putative peptidoglycan-binding (PGB) domain in the periplasmic region of PomB (PomB(C)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00113-11

    authors: Li N,Kojima S,Homma M

    更新日期:2011-08-01 00:00:00

  • Development and trifoliin A-binding ability of the capsule of Rhizobium trifolii.

    abstract::The age-dependent lectin-binding ability of Rhizobium trifolii 0403 capsular polysaccharide (CPS) was examined by following the development of the capsule and its ability to interact with the white clover lectin trifoliin A. Bacteria grown on agar plates for 3, 5, 7, 14, and 21 days were examined by electron microscop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.145-152.1984

    authors: Sherwood JE,Vasse JM,Dazzo FB,Truchet GL

    更新日期:1984-07-01 00:00:00

  • Regulation of sialic acid metabolism in Escherichia coli: role of N-acylneuraminate pyruvate-lyase.

    abstract::In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.854-860.1985

    authors: Vimr ER,Troy FA

    更新日期:1985-11-01 00:00:00

  • Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation.

    abstract::When allyl alcohol was used as a suicide substrate, spontaneous mutants and UV light- and nitrous acid-generated mutants of Methylobacterium organophilum XX were selected which grew on methylamine but not on methanol. There was no detectable methanol dehydrogenase (MDH) activity in crude extracts of these mutants, yet...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.141-148.1988

    authors: Machlin SM,Tam PE,Bastien CA,Hanson RS

    更新日期:1988-01-01 00:00:00

  • Positive and negative control of ompB transcription in Escherichia coli by cyclic AMP and the cyclic AMP receptor protein.

    abstract::The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an omp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.3.664-670.1992

    authors: Huang L,Tsui P,Freundlich M

    更新日期:1992-02-01 00:00:00

  • Complementation of the lytD1 mutation of Escherichia coli by either the cI or cro gene of bacteriophage lambda.

    abstract::The lytD1 mutant of Escherichia coli exhibits temperature-sensitive growth which is attributed to cellular autolysis at the restrictive temperature. Either of two cloned phage lambda genes, identified as cI and cro, suppressed the lytD1(Ts) lysis phenotype, suggesting that lytD encodes a DNA-binding protein with a DNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.893-895.1991

    authors: Dai DX,Ishiguro EE

    更新日期:1991-01-01 00:00:00

  • EVIDENCE OF LYSOGENY IN DERIVATIVES OF ESCHERICHIA COLI.

    abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.446-452.1965

    authors: FRAMPTON EW,BRINKLEY BR

    更新日期:1965-08-01 00:00:00

  • Signal transduction protein PII phosphatase PphA is required for light-dependent control of nitrate utilization in synechocystis sp. strain PCC 6803.

    abstract::Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.19.6683-6690.2005

    authors: Kloft N,Forchhammer K

    更新日期:2005-10-01 00:00:00

  • Highly conjugative pMG1-like plasmids carrying Tn1546-like transposons that encode vancomycin resistance in Enterococcus faecium.

    abstract::A total of 12 VanA-type vancomycin-resistant enterococci, consisting of 10 Enterococcus faecium isolates and two Enterococcus avium isolates, were examined in detail. The vancomycin resistance conjugative plasmids pHTalpha (65.9 kbp), pHTbeta (63.7 kbp), and pHTgamma (66.5 kbp) were isolated from each of three differe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.23.7024-7028.2003

    authors: Tomita H,Tanimoto K,Hayakawa S,Morinaga K,Ezaki K,Oshima H,Ike Y

    更新日期:2003-12-01 00:00:00

  • Replicative deoxyribonucleic acid synthesis in isolated mitochondria from Saccharomyces cerevisiae.

    abstract::The characteristics of a system for the in vitro synthesis of mitochondrial deoxyribonucleic acid (mtDNA) in mitochondria isolated from Saccharomyces cerevisiae are described. In this system the exclusive product of the reaction is mtDNA. Under optimal conditions the initial rate of synthesis is close to the calculate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.973-982.1977

    authors: Mattick JS,Hall RM

    更新日期:1977-06-01 00:00:00

  • A naturally occurring gene amplification leading to sulfonamide and trimethoprim resistance in Streptococcus agalactiae.

    abstract::Gene amplifications have been detected as a transitory phenomenon in bacterial cultures. They are predicted to contribute to rapid adaptation by simultaneously increasing the expression of genes clustered on the chromosome. However, genome amplifications have rarely been described in natural isolates. Through DNA arra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01357-07

    authors: Brochet M,Couvé E,Zouine M,Poyart C,Glaser P

    更新日期:2008-01-01 00:00:00

  • Xenorhabdus nematophilus as a model for host-bacterium interactions: rpoS is necessary for mutualism with nematodes.

    abstract::Xenorhabdus nematophilus, a gram-negative bacterium, is a mutualist of Steinernema carpocapsae nematodes and a pathogen of larval-stage insects. We use this organism as a model of host-microbe interactions to identify the functions bacteria require for mutualism, pathogenesis, or both. In many gram-negative bacteria, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.16.4687-4693.2001

    authors: Vivas EI,Goodrich-Blair H

    更新日期:2001-08-01 00:00:00

  • Characterization of the ccpA gene of Enterococcus faecalis: identification of starvation-inducible proteins regulated by ccpA.

    abstract::Inactivation of ccpA in Enterococcus faecalis leads to reduction of the growth rate, derepression of the galKETR operon in the presence of a mixture of glucose and galactose, and reduction of transcription of ldh in the presence of glucose. Moreover, the E. faecalis ccpA gene fully complements a Bacillus subtilis ccpA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.20.5799-5806.2000

    authors: Leboeuf C,Leblanc L,Auffray Y,Hartke A

    更新日期:2000-10-01 00:00:00