Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts.

abstract：:Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...

journal_title：Journal of bacteriology

authors： Skurray RA,Reeves P

更新日期：1974-01-01 00:00:00

abstract：:The major acid-soluble spore proteins (ASSPs) of Bacillus subtilis were detected by immunoprecipitation of radioactively labeled in vitro- and in vivo-synthesized proteins. ASSP synthesis in vivo began 2 h after the initiation of sporulation (t2) and reached its maximum rate at t7. This corresponded to the time of syn...

journal_title：Journal of bacteriology

authors： Leventhal JM,Chambliss GH

更新日期：1982-12-01 00:00:00

abstract：:A Pseudomonas strain produced an enzyme capable of deacylating 7 beta-(4-carboxybutanamido)cephalosporanic acid to 7-aminocephalosporanic acid in response to glutaric acid. The gene for the enzyme was cloned within the PstI site of pBR325 as a 7.35-kilobase-pair DNA segment from a mutant of this strain whose enzyme is...

journal_title：Journal of bacteriology

authors： Matsuda A,Komatsu KI

更新日期：1985-09-01 00:00:00

abstract：:Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...

journal_title：Journal of bacteriology

authors： Boehm M,Nield J,Zhang P,Aro EM,Komenda J,Nixon PJ

更新日期：2009-10-01 00:00:00

abstract：:Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...

journal_title：Journal of bacteriology

authors： Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

更新日期：1998-06-01 00:00:00

abstract：:Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

journal_title：Journal of bacteriology

authors： Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

更新日期：2019-06-21 00:00:00

abstract：:Glucose kinase is required for glucose repression of several catabolite-controlled genes in Streptomyces coelicolor and certainly plays an important role in glucose repression in these organisms. We report here that glucose kinase null mutants of S. coelicolor retain transcriptional regulation of the chitinase 63 gene...

journal_title：Journal of bacteriology

authors： Ingram C,Westpheling J

更新日期：1995-06-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 can fix N(2) under oxic conditions, and the activity of nitrogen fixation occurs exclusively in heterocysts, cells differentiated from vegetative cells in response to a limitation of a combined-nitrogen source in the growth medium. At the late stages of heter...

journal_title：Journal of bacteriology

authors： Shi L,Li JH,Cheng Y,Wang L,Chen WL,Zhang CC

更新日期：2007-07-01 00:00:00

abstract：:We present the complete genome sequence of Yersinia pestis KIM, the etiologic agent of bubonic and pneumonic plague. The strain KIM, biovar Mediaevalis, is associated with the second pandemic, including the Black Death. The 4.6-Mb genome encodes 4,198 open reading frames (ORFs). The origin, terminus, and most genes en...

journal_title：Journal of bacteriology

authors： Deng W,Burland V,Plunkett G 3rd,Boutin A,Mayhew GF,Liss P,Perna NT,Rose DJ,Mau B,Zhou S,Schwartz DC,Fetherston JD,Lindler LE,Brubaker RR,Plano GV,Straley SC,McDonough KA,Nilles ML,Matson JS,Blattner FR,Perry RD

更新日期：2002-08-01 00:00:00

abstract：:The histidine kinase (HK) component of many two-component regulatory systems exhibits regulated ability to phosphorylate itself and to participate in transfer of phosphate to and from its cognate response regulator. The signaling system that controls expression of the UhpT sugar phosphate transporter in Escherichia co...

journal_title：Journal of bacteriology

authors： Wright JS,Olekhnovich IN,Touchie G,Kadner RJ

更新日期：2000-11-01 00:00:00

abstract：:Methanococcus aeolicus, Methanococcus maripaludis, and Methanococcus voltae contain similar levels of four enzymes of branched-chain amino acid biosynthesis: acetohydroxy acid synthase, acetohydroxy acid isomeroreductase, dihydroxy acid dehydratase, and transaminase B. Following growth at low partial pressures of H2-C...

journal_title：Journal of bacteriology

authors： Xing RY,Whitman WB

更新日期：1991-03-01 00:00:00

abstract：:The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...

journal_title：Journal of bacteriology

authors： Domelier AS,van der Mee-Marquet N,Sizaret PY,Héry-Arnaud G,Lartigue MF,Mereghetti L,Quentin R

更新日期：2009-08-01 00:00:00

abstract：:Two of the three plasmids of the wild-type Rhizobium meliloti 41 (pRme41a and pRme41c) carry a copy of ISRm2, a 2.7-kilobase-long transposable element. ISRm2 is terminated by 22-base-pair (bp) inverted repeat sequences, exhibiting some homology to the inverted repeats of elements generating 9-bp target sequence duplic...

journal_title：Journal of bacteriology

authors： Dusha I,Kovalenko S,Banfalvi Z,Kondorosi A

更新日期：1987-04-01 00:00:00

abstract：:We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...

journal_title：Journal of bacteriology

authors： Park JT,Mahadevan S

更新日期：1988-08-01 00:00:00

abstract：:Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with ...

journal_title：Journal of bacteriology

authors： Wehr CT,Waskell L,Glaser DA

更新日期：1975-01-01 00:00:00

abstract：:A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...

journal_title：Journal of bacteriology

authors： Yang GC,Schrank GD,Freeman BA

更新日期：1977-02-01 00:00:00

abstract：:Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

journal_title：Journal of bacteriology

authors： Arnold WN,Garrison RG

更新日期：1979-03-01 00:00:00

abstract：:Both the acridine half-mustard, ICR191, and the nonalkylating azaacridine derivative, ICR364-OH, induce three classes of frameshift mutations in the histidine operon of Salmonella typhimurium. (i) One class is completely stable in reversion tests and is presumed to represent deletion of one or a few critical nucleotid...

journal_title：Journal of bacteriology

authors： Oeschger NS,Hartman PE

更新日期：1970-02-01 00:00:00

abstract：:The gene for the catabolic NAD-linked glutamate dehydrogenase of Peptostreptococcus asaccharolyticus was cloned by selection of Escherichia coli for complementation of a biosynthetic defect. Cloned fragments containing the gene and the P. asaccharolyticus transcription and translation signals are very highly expressed...

journal_title：Journal of bacteriology

authors： Snedecor B,Chu H,Chen E

更新日期：1991-10-01 00:00:00

abstract：:The first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a c...

journal_title：Journal of bacteriology

authors： Vary PS,Garbe JC,Franzen M,Frampton EW

更新日期：1982-03-01 00:00:00

abstract：:The virulence factors of the cariogenic bacterium Streptococcus sobrinus have been difficult to assess because of a lack of tools for the genetic manipulation of this organism. The construction of an Escherichia coli-Streptococcus shuttle vector, pDL289, that can be mobilized into S. sobrinus by the conjugative plasmi...

journal_title：Journal of bacteriology

authors： Buckley ND,Lee LN,LeBlanc DJ

更新日期：1995-09-01 00:00:00

abstract：:The experiments presented in this paper suggest that the shift observed in sedimentation of deoxyribonucleic acid from cells of Escherichia coli subjected to amino acid starvation is related to inhibition of ribonucleic acid synthesis rather than to its release from the membrane at the termination of replication. ...

journal_title：Journal of bacteriology

authors： Yaffe E,Grossman N,Ron EZ

更新日期：1979-02-01 00:00:00

abstract：:The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...

journal_title：Journal of bacteriology

authors： Lafontaine ER,Cope LD,Aebi C,Latimer JL,McCracken GH Jr,Hansen EJ

更新日期：2000-03-01 00:00:00

abstract：:Nearly every known species of Eubacteria encodes a homolog of the Borrelia burgdorferi EbfC DNA-binding protein. We now demonstrate that fluorescently tagged EbfC associates with B. burgdorferi nucleoids in vivo and that chromatin immunoprecipitation (ChIP) of wild-type EbfC showed it to bind in vivo to sites througho...

journal_title：Journal of bacteriology

authors： Jutras BL,Bowman A,Brissette CA,Adams CA,Verma A,Chenail AM,Stevenson B

更新日期：2012-07-01 00:00:00

abstract：:Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage u...

journal_title：Journal of bacteriology

authors： Pickard D,Thomson NR,Baker S,Wain J,Pardo M,Goulding D,Hamlin N,Choudhary J,Threfall J,Dougan G

更新日期：2008-04-01 00:00:00

abstract：:The Escherichia coli K-12 hisT gene has been cloned, and its organization and expression have been analyzed on multicopy plasmids. The hisT gene, which encodes tRNA pseudouridine synthase I (PSUI), was isolated on a Clarke-Carbon plasmid known to contain the purF gene. The presence of the hisT gene on this plasmid was...

journal_title：Journal of bacteriology

authors： Marvel CC,Arps PJ,Rubin BC,Kammen HO,Penhoet EE,Winkler ME

更新日期：1985-01-01 00:00:00

abstract：:XbaI digestion and pulsed-field gel electrophoresis of the genome of Salmonella typhimurium LT2 yields 24 fragments: 23 fragments (total size, 4,807 kb) are from the chromosome, and one fragment (90 kb) is from the virulence plasmid pSLT. Some of the 23 fragments from the chromosome were located on the linkage map by ...

journal_title：Journal of bacteriology

authors： Liu SL,Sanderson KE

更新日期：1992-03-01 00:00:00

abstract：:Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be posttranslationally modified for full functionality; in enterobacteria, this is accomplished by two enzymes, namely, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a...

journal_title：Journal of bacteriology

authors： Klee SM,Sinn JP,Holmes AC,Lehman BL,Krawczyk T,Peter KA,McNellis TW

更新日期：2019-05-08 00:00:00

abstract：:Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat inno...

journal_title：Journal of bacteriology

authors： Kondo I,Ishikawa T,Nakahara H

更新日期：1974-01-01 00:00:00

abstract：:In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

journal_title：Journal of bacteriology

authors： Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

更新日期：2017-05-09 00:00:00