Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp.

abstract：:Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

journal_title：Journal of bacteriology

authors： Garcia DL,Dillard JP

更新日期：2008-06-01 00:00:00

abstract：:Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...

journal_title：Journal of bacteriology

authors： Solbiati JO,Ciaccio M,Farías RN,Salomón RA

更新日期：1996-06-01 00:00:00

abstract：:Lipopolysaccharide (LPS) fractions were isolated from three species of blue-green algae of the genus Phormidium, namely, P. africanum, P. laminosum, and P. uncinatum, by using a phenol-water procedure followed by exhaustive extraction with ammonium oxalate. The materials obtained were shown to be closely related bioch...

journal_title：Journal of bacteriology

authors： Mikheyskaya LV,Ovodova RG,Ovodov YS

更新日期：1977-04-01 00:00:00

abstract：:A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1973-05-01 00:00:00

abstract：:The gonococcal lsi-6 locus was cloned and shown by DNA sequence analysis to have homology with the E. coli rfaD gene, which encodes ADP-L-glycero-D-mannoheptose epimerase. This enzyme is involved in the biosynthesis of the lipopolysaccharide precursor ADP-L-glycero-D-mannoheptose. A site-directed frameshift mutation i...

journal_title：Journal of bacteriology

authors： Drazek ES,Stein DC,Deal CD

更新日期：1995-05-01 00:00:00

abstract：:The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...

journal_title：Journal of bacteriology

authors： Götz R,Schmitt R

更新日期：1987-07-01 00:00:00

abstract：:Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

journal_title：Journal of bacteriology

authors： Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

更新日期：1998-04-01 00:00:00

abstract：:In a horse serum-based medium containing a full complement of fatty acids, cells of Spiroplasma citri were seen to preferentially incorporate palmitic acid. In the same medium, which had a steryl ester-to-sterol ratio of 3.64, a steryl ester-to-sterol ratio of 0.23 was seen in the cells, cholesterol being preferential...

journal_title：Journal of bacteriology

authors： Freeman BA,Sissenstein R,McManus TT,Woodward JE,Lee IM,Mudd JB

更新日期：1976-03-01 00:00:00

abstract：:The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

journal_title：Journal of bacteriology

authors： Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

更新日期：2019-11-20 00:00:00

abstract：:The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...

journal_title：Journal of bacteriology

authors： White WB,Ferry JG

更新日期：1992-08-01 00:00:00

abstract：:Lysozyme Chalaropsis, a beta-1,4-N,6-O-diacetylmuramidase, attacks the cell wall murein of Staphylococcus aureus H in a random manner. In hypertonic sucrose solutions, true protoplasts are produced and, in the process, mesosomal tubules are extruded into the medium. ...

journal_title：Journal of bacteriology

authors： Robinson JP,Robinson RD,Hash JH

更新日期：1974-02-01 00:00:00

abstract：:Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed fro...

journal_title：Journal of bacteriology

authors： Brøndsted L,Atlung T

更新日期：1994-09-01 00:00:00

abstract：:Ellar, D. J. (Syracuse University, Syracuse, N.Y.), and D. G. Lundgren. Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium. J. Bacteriol. 92:1748-1764. 1966.-A study was made of the fine structure of sporulating cells of Bacillus cereus grown in a chemically defined medium. The devel...

journal_title：Journal of bacteriology

authors： Ellar DJ,Lundgren DG

更新日期：1966-12-01 00:00:00

abstract：:Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...

journal_title：Journal of bacteriology

authors： Summers RG,Wendt-Pienkowski E,Motamedi H,Hutchinson CR

更新日期：1992-03-01 00:00:00

abstract：:Flagellum formation by ubiquinone- and menaquinone-deficient mutant strains of Escherichia coli K-12 was studied under both aerobic and anaerobic growth conditions. Ubiquinone was found to be obligatory for aerobic flagellum formation but could be replaced by menaquinone for anaerobic flagellum formation. A mutant dev...

journal_title：Journal of bacteriology

authors： Hertz R,Bar-Tana J

更新日期：1977-12-01 00:00:00

abstract：:A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

journal_title：Journal of bacteriology

authors： Taylor BF,Campbell WL,Chinoy I

更新日期：1970-05-01 00:00:00

abstract：:The terminal oxidase content of Bacillus firmus OF4, a facultative alkaliphile that grows well over the pH range of 7.5 to 10.5, was studied by difference spectroscopy. Evidence was found for three terminal oxidases under different growth conditions. The growth pH and the stage of growth profoundly affected the expres...

journal_title：Journal of bacteriology

authors： Hicks DB,Plass RJ,Quirk PG

更新日期：1991-08-01 00:00:00

abstract：:Insertion-duplication mutagenesis was used to generate mutants of Streptococcus pneumoniae that produced truncated forms of PspA (pneumococcal surface protein A). The truncated products, representing from 20 to 80% of the complete PspA molecule, were all secreted from the cell and could be detected in unconcentrated c...

journal_title：Journal of bacteriology

authors： Yother J,Handsome GL,Briles DE

更新日期：1992-01-01 00:00:00

abstract：:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

journal_title：Journal of bacteriology

authors： Hartmann RK,Toschka HY,Erdmann VA

更新日期：1991-04-01 00:00:00

abstract：:The production of extracellular alpha-amylase and protease by protoplasts of Bacillus amyloliquefaciens has been achieved. The production of enzymically active protease was totally dependent on a high concentration of either Mg2+, Ca2+, or spermidine, but production of active alpha-amylase was not. This cation depende...

journal_title：Journal of bacteriology

authors： Sanders RL,May BK

更新日期：1975-09-01 00:00:00

abstract：:The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

journal_title：Journal of bacteriology

authors： Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

更新日期：1975-11-01 00:00:00

abstract：:The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...

journal_title：Journal of bacteriology

authors： Ellermeier JR,Slauch JM

更新日期：2008-01-01 00:00:00

abstract：:The structural properties required for the binding of peptide substrates to the Escherichia coli periplasmic protein involved in oligopeptide transport were surveyed by measuring the ability of different peptides to compete for binding in an equilibrium dialysis assay with the tripeptide Ala-Phe-[3H]Gly. The protein s...

journal_title：Journal of bacteriology

authors： Guyer CA,Morgan DG,Staros JV

更新日期：1986-11-01 00:00:00

abstract：:The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

journal_title：Journal of bacteriology

authors： de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

更新日期：1989-12-01 00:00:00

abstract：:Gaffar, Abdul (Brigham Young University, Provo, Utah), David R. Terry, and Richard D. Sagers. Amino acid composition of walls from single and filamentous cells of Clostridium acidiurici. J. Bacteriol. 91:1618-1624. 1966.-The walls from single and filamentous cells of Clostridium acidiurici were shown to contain 11 ami...

journal_title：Journal of bacteriology

authors： Gaffar A,Terry DR,Sagers RD

更新日期：1966-04-01 00:00:00

abstract：:High-affinity iron acquisition in Bacillus subtilis is mediated via the bacillibactin catechole siderophore pathway. Three of the four essential pathway steps, bacillibactin synthesis, Fe-bacillibactin uptake, and Fe-bacillibactin hydrolysis have been characterized previously. The functional and regulatory components ...

journal_title：Journal of bacteriology

authors： Miethke M,Schmidt S,Marahiel MA

更新日期：2008-08-01 00:00:00

abstract：:Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

journal_title：Journal of bacteriology

authors： Kumar S

更新日期：1976-02-01 00:00:00

abstract：:Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

journal_title：Journal of bacteriology

authors： Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

更新日期：2006-08-01 00:00:00

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00

abstract：:When Methanobacterium thermoautotrophicum cells were incubated in 50 mM potassium phosphate buffer (pH 7.0) containing 1 M sucrose and autolysate from Methanobacterium wolfei, they were transformed into protoplasts. The protoplasts, which possessed no cell wall, lysed in buffer without sucrose. Unlike whole cells, the...

journal_title：Journal of bacteriology

authors： Mountfort DO,Mörschel E,Beimborn DB,Schönheit P

更新日期：1986-11-01 00:00:00