Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.

Abstract:

:A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent (Q(10) between 27 C and 37 C was about 2), energy-dependent, and pH-dependent and was inhibited by iodoacetate, 2,4-dinitrophenol, potassium fluoride, and sodium azide. The uptake obeyed saturation kinetics with an apparent K(m) of 6.6 x 10(-6) M and V(max) of 4.0 x 10(-10) mol per min per mg (dry weight). At the steady state the intracellular concentration of PteGlu was 120-fold higher from that of the medium. Reduced folates like 5-CHO-H(4)PteGlu and methyl-tetrahydrofolate (5-CH(3)-H(4)PteGlu) as well as 2,4-diaminoanalogues (amethopterin and aminopterin) were shown to compete for the PteGlue-carrier.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mandelbaum-Shavit F,Grossowicz N

doi

10.1128/JB.114.2.485-490.1973

subject

Has Abstract

pub_date

1973-05-01 00:00:00

pages

485-90

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

114

pub_type

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