Evidence for extrusion of unfolded extracellular enzyme polypeptide chains through membranes of Bacillus amyloliquefaciens.

abstract：:A 6.2-kb region of DNA corresponding to complementation groups II and III of the Erwinia amylovora hrp gene cluster was analyzed. Transposon mutagenesis indicated that the two complementation groups are required for secretion of harpin, an elicitor of the hypersensitive reaction. The sequence of the region revealed 10...

journal_title：Journal of bacteriology

authors： Kim JF,Wei ZM,Beer SV

更新日期：1997-03-01 00:00:00

abstract：:We used cells carrying plasmids causing the overproduction of Gln3p, Ure2p, or both of these proteins to elucidate the ability of Ure2p to prevent the activation of gene expression by Gln3p in cells growing in a glutamine-containing medium. Our results indicate that Ure2p probably does not interfere with the binding o...

journal_title：Journal of bacteriology

authors： Blinder D,Coschigano PW,Magasanik B

更新日期：1996-08-01 00:00:00

abstract：:The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and crystallized. A temperat...

journal_title：Journal of bacteriology

authors： Dallas WS,Gowen JE,Ray PH,Cox MJ,Dev IK

更新日期：1992-09-01 00:00:00

abstract：:Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...

journal_title：Journal of bacteriology

authors： Snow R

更新日期：1967-09-01 00:00:00

abstract：:Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

journal_title：Journal of bacteriology

authors： Friedberg I,Avigad G

更新日期：1968-08-01 00:00:00

abstract：:The ability of Clostridium perfringens type A to produce an enterotoxin active in human food poisoning has been shown to be directly related to the ability of the organism to sporulate. Enterotoxin was produced only in a sporulation medium and not in a growth medium in which sporulation was repressed. Mutants with an ...

journal_title：Journal of bacteriology

authors： Duncan CL,Strong DH,Sebald M

更新日期：1972-04-01 00:00:00

abstract：:The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...

journal_title：Journal of bacteriology

authors： Bjorn MJ,Sokol PA,Iglewski BH

更新日期：1979-04-01 00:00:00

abstract：:A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...

journal_title：Journal of bacteriology

authors： Sabaty M,Kaplan S

更新日期：1996-01-01 00:00:00

abstract：:DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...

journal_title：Journal of bacteriology

authors： Vujaklija D,Horinouchi S,Beppu T

更新日期：1993-05-01 00:00:00

abstract：:Klebsiella pneumoniae is a nosocomial pathogen frequently isolated from opportunistic infections, especially in clinical environments. In spite of its potential pathogenicity, this microorganism has several metabolic potentials that could be used in biotechnology applications. K. pneumoniae is able to metabolize glyce...

journal_title：Journal of bacteriology

authors： Marçal D,Rêgo AT,Carrondo MA,Enguita FJ

更新日期：2009-02-01 00:00:00

abstract：:Form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of the Calvin-Benson-Bassham cycle may be divided into two broad phylogenetic groups, referred to as red-like and green-like, based on deduced large subunit amino acid sequences. Unlike the form I enzyme from the closely related organism Rhodobacter spha...

journal_title：Journal of bacteriology

authors： Horken KM,Tabita FR

更新日期：1999-07-01 00:00:00

abstract：:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

journal_title：Journal of bacteriology

authors： Huang AM,Lee JI,King SC,Wilson TH

更新日期：1992-08-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was pur...

journal_title：Journal of bacteriology

authors： Walderich B,Höltje JV

更新日期：1989-06-01 00:00:00

abstract：:Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...

journal_title：Journal of bacteriology

authors： Massa SM,Sharma AD,Siletti C,Tu Z,Godfrey JJ,Gutheil WG,Huynh TN

更新日期：2020-11-19 00:00:00

abstract：:Rhodococcus sp. strain DK17 is capable of utilizing various derivatives of benzene and bicyclics containing both aromatic and alicyclic moieties as sole carbon and energy sources. Here, we present the 9,107,362-bp draft genome sequence of DK17 and its genomic analysis in comparison with other members of the genus Rhod...

journal_title：Journal of bacteriology

authors： Yoo M,Kim D,Choi KY,Chae JC,Zylstra GJ,Kim E

更新日期：2012-08-01 00:00:00

abstract：UNLABELLED:Resistance to methicillin and other β-lactam antibiotics in staphylococci is due to mecA, which is carried on a genomic island, staphylococcal cassette chromosome mec (SCCmec). The chromosomal excision and integration of SCCmec are mediated by the site-specific recombinase CcrAB or CcrC, encoded within this ...

journal_title：Journal of bacteriology

authors： Wang L,Ahmed MH,Safo MK,Archer GL

更新日期：2015-09-01 00:00:00

abstract：:Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

journal_title：Journal of bacteriology

authors： Ma Z,Masuda N,Foster JW

更新日期：2004-11-01 00:00:00

abstract：:Ruthenium red stained a capsule external to the exine of the Azotobacter cyst. The central body is therefore surrounded by three layers, the intine, the exine, and the capsule, all containing acid mucopolysaccharide. Vesicles that appear to originate from the contracting cell membrane of the central body may account f...

journal_title：Journal of bacteriology

authors： Pope LM,Wyss O

更新日期：1970-04-01 00:00:00

abstract：:We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...

journal_title：Journal of bacteriology

authors： Freifelder D,Folkmanis A,Kirschner I

更新日期：1971-03-01 00:00:00

abstract：:By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...

journal_title：Journal of bacteriology

authors： Vermeulen CA,Venema G

更新日期：1974-05-01 00:00:00

abstract：:Derivatives of Pseudomonas sp. B13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the TOL plasmid of Pseudomonas putida mt-2 were studied. The utilization of these substrates, a property not shared by the parent strains, was shown to dep...

journal_title：Journal of bacteriology

authors： Reineke W,Knackmuss HJ

更新日期：1980-05-01 00:00:00

abstract：:Biodegradation of metal-citrate complexes by Pseudomonas fluorescens depends on the nature of the complex formed between the metal and citric acid. Bidentate Fe(III)-, Ni-, and Zn-citrate complexes were readily biodegraded, but the tridentate Cd- and Cu-citrate, and U-citrate complexes were not. The biodegradation of ...

journal_title：Journal of bacteriology

authors： Joshi-Tope G,Francis AJ

更新日期：1995-04-01 00:00:00

abstract：:Virulent bacteria of the genus Yersinia secrete a number of virulence determinants called Yops. These proteins lack typical signal sequences and are not posttranslationally processed. Two gene loci have been identified as being involved in the specific Yop secretion system (G. Cornelis, p. 231-265, In C. E. Hormache, ...

journal_title：Journal of bacteriology

authors： Bergman T,Erickson K,Galyov E,Persson C,Wolf-Watz H

更新日期：1994-05-01 00:00:00

abstract：:Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...

journal_title：Journal of bacteriology

authors： Conrad B,Bashkirov VI,Hofemeister J

更新日期：1992-11-01 00:00:00

abstract：:The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of beta-mercaptoethanol or incubated with thiol-specific reagents. The enzyme was protected from inactivation in the presence ...

journal_title：Journal of bacteriology

authors： Pompeo F,van Heijenoort J,Mengin-Lecreulx D

更新日期：1998-09-01 00:00:00

abstract：:Cyclic adenosine 3',5'-monophosphate isolated from Mycobacterium smegmatis cells was identified by thin-layer chromatography, stepwise conversion to adenosine 5'-monophosphate and adenosine, ultraviolet absorption spectrum, phosphate analysis, and detection by two relatively specific radioisotopic methods. ...

journal_title：Journal of bacteriology

authors： Lee CH

更新日期：1977-12-01 00:00:00

abstract：:In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...

journal_title：Journal of bacteriology

authors： Suzuki T,Hitomi A,Magee PT,Sakaguchi S

更新日期：1994-06-01 00:00:00

abstract：:The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...

journal_title：Journal of bacteriology

authors： Ricca E,Limauro D,Lago CT,de Felice M

更新日期：1988-11-01 00:00:00

abstract：:Transcriptional factor CarD is the only reported prokaryotic analog of eukaryotic high-mobility-group A (HMGA) proteins, in that it has contiguous acidic and AT hook DNA-binding segments and multifunctional roles in Myxococcus xanthus carotenogenesis and fruiting body formation. HMGA proteins are small, randomly struc...

journal_title：Journal of bacteriology

authors： Cayuela ML,Elías-Arnanz M,Peñalver-Mellado M,Padmanabhan S,Murillo FJ

更新日期：2003-06-01 00:00:00