Abstract:
:The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end product inhibition by valine, also increased its specific activity and lowered the Km for alpha-acetolactate synthesis. The mutation increased the sensitivity of acetohydroxy acid synthase III to dialysis and heat treatment and reduced the requirement for thiamine pyrophosphate addition to the assay mixture for activity. A strain carrying the ilvH612 mutation grew better than a homologous ilvH+ strain in the presence of leucine. The data indicate that this is a consequence of a more active acetohydroxy acid synthase III isozyme rather than the result of an alteration of the leucine-mediated repression of the ilvIH operon.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ricca E,Limauro D,Lago CT,de Felice Mdoi
10.1128/jb.170.11.5197-5199.1988subject
Has Abstractpub_date
1988-11-01 00:00:00pages
5197-9issue
11eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.4071-4074.1997
更新日期:1997-06-01 00:00:00
abstract::SecA facilitates protein transport across the eubacterial plasma membrane by its association with cargo proteins and the SecYEG translocon, followed by ATP-driven conformational changes that promote protein translocation in a stepwise manner. Whether SecA functions as a monomer or a dimer during this process has been ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.335-338.2006
更新日期:2006-01-01 00:00:00
abstract::The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same cu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.100-104.1986
更新日期:1986-04-01 00:00:00
abstract::Finnerty, W. R. (University of Iowa, Iowa City), and R. E. Kallio. Origin of palmitic acid carbon in palmitates formed from hexadecane-1-C(14) and tetradecane-1-C(14) by Micrococcus cerificans. J. Bacteriol. 87:1261-1265. 1964.-Degradation of the palmitic acid moiety of cetyl palmitate and myristyl palmitate formed fr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.6.1261-1265.1964
更新日期:1964-06-01 00:00:00
abstract::We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.389-398.1997
更新日期:1997-01-01 00:00:00
abstract::Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00747-09
更新日期:2009-11-01 00:00:00
abstract::Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.3.677-683.1997
更新日期:1997-02-01 00:00:00
abstract::Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcripti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00868-06
更新日期:2006-11-01 00:00:00
abstract::Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535-545. 1966.-Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.535-545.1966
更新日期:1966-02-01 00:00:00
abstract::The propanediol utilization (pdu) operon of Salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme B12-dependent propanediol dehydratase. A clone that expresses propanediol dehydratase activity was isolated from a Salmonella genomic library. DNA sequence analysis showe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6633-6639.1997
更新日期:1997-11-01 00:00:00
abstract::Pseudomonas aeruginosa and members of the Burkholderia cepacia complex often coexist in both the soil and the lungs of cystic fibrosis patients. To gain an understanding of how these different species affect each other's physiology when coexisting, we performed a screen to identify P. aeruginosa genes that are induced...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.8.2376-2384.2004
更新日期:2004-04-01 00:00:00
abstract::During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.7.2372-2375.2001
更新日期:2001-04-01 00:00:00
abstract::Bap (biofilm-associated protein) is a 254-kDa staphylococcal surface protein implicated in formation of biofilms by staphylococci isolated from chronic mastitis infections. The presence of potential EF-hand motifs in the amino acid sequence of Bap prompted us to investigate the effect of calcium on the multicellular b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7490-7498.2004
更新日期:2004-11-01 00:00:00
abstract::Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.384-389.1985
更新日期:1985-10-01 00:00:00
abstract::We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3447-3456.1996
更新日期:1996-06-01 00:00:00
abstract::A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade thes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3897-3902.1988
更新日期:1988-09-01 00:00:00
abstract::A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.500-508.1981
更新日期:1981-08-01 00:00:00
abstract::The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green fluorescent protein (GFP) in the cytoplasm of Escherichia coli. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.197-203.1999
更新日期:1999-01-01 00:00:00
abstract::Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00477-09
更新日期:2009-09-01 00:00:00
abstract::The lasA gene (whose product is involved in the production of extracellular elastolytic activity) was isolated from a genomic bank containing DNA from Pseudomonas aeruginosa FRD1. Recombinant plasmid pELA1, containing the lasA gene, complemented the temperature-sensitive elastase mutation (lasA1) in P. aeruginosa PAO-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1349-1351.1987
更新日期:1987-03-01 00:00:00
abstract::Silicibacter sp. strain TM1040, a member of the Roseobacter clade, forms a symbiosis with unicellular phytoplankton, which is inextricably linked to the biphasic "swim or stick" lifestyle of the bacteria. Mutations in flaC bias the population toward the motile phase. Renewed examination of the FlaC(-) strain (HG1016) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01777-12
更新日期:2013-02-01 00:00:00
abstract::Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.758-769.1980
更新日期:1980-02-01 00:00:00
abstract::Falcone, Giuseppe (Rutgers, The State University, New Brunswick, N.J.), and Walter J. Nickerson. Reduction of selenite by intact yeast cells and cell-free preparations. J. Bacteriol. 85:754-762. 1963.-Nonproliferating cell suspensions of Candida albicans rapidly reduced selenite to red, metallic selenium in the absenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.754-762.1963
更新日期:1963-04-01 00:00:00
abstract::Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analys...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4591-4598.1997
更新日期:1997-07-01 00:00:00
abstract::An increase in the apparent molecular weight of the principal outer membrane protein (POMP) of Neisseria gonorrhoeae is associated with introduction of the penB2 genetic marker, which results in low-level, relatively nonspecific antibiotic resistance. Limited proteolysis of the two forms of POMP showed that they had f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.847-851.1980
更新日期:1980-08-01 00:00:00
abstract::Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.22.7857-7862.2005
更新日期:2005-11-01 00:00:00
abstract::The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.541-543.1993
更新日期:1993-01-01 00:00:00
abstract::The reoxidation of NADH generated in reactions within the mitochondrial matrix of Saccharomyces cerevisiae is catalyzed by an NADH dehydrogenase designated Ndi1p (C. A. M. Marres, S. de Vries, and L. A. Grivell, Eur. J. Biochem. 195:857-862, 1991). Gene disruption analysis was used to examine possible metabolic functi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.16.4051-4055.1998
更新日期:1998-08-01 00:00:00
abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.153-160.1979
更新日期:1979-01-01 00:00:00
abstract::We purified membrane-bound cytochrome c-550 [cytochrome c-550(m)] to an electrophoretically homogeneous state from Nitrobacter winogradskyi. The cytochrome showed peaks at 409 and 525 nm in the oxidized form and peaks at 416, 521, and 550 nm in the reduced form. The molecular weight of the cytochrome was estimated to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.14.4400-4404.1993
更新日期:1993-07-01 00:00:00