cse15, cse60, and csk22 are new members of mother-cell-specific sporulation regulons in Bacillus subtilis.

Abstract:

:We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requirement for sigmaE. Maximal expression of both cse15 and cse60 further depended on the DNA-binding protein SpoIIID. Primer extension results revealed -10 and -35 sequences upstream of the cse15 and cse60 coding sequences very similar to those utilized by sigmaE-containing RNA polymerase. Alignment of these and other regulatory regions led to a revised consensus sequence for sigmaE-dependent promoters. A third transcriptional unit, designated csk22, was localized at approximately 173 degrees on the chromosome. Transcription of csk22 was activated at h 4 of sporulation, required the late mother-cell regulator sigmaK, and was repressed by the GerE protein. Sequences in the csk22 promoter region were similar to those of other sigmaK-dependent promoters. The cse60 locus was deduced to encode an acidic product of only 60 residues. A 37.6-kDa protein apparently encoded by cse15 was weakly related to the heavy chain of myosins, as well as to other myosin-like proteins, and is predicted to contain a central, 100 residue-long coiled-coil domain. Finally, csk22 is inferred to encode a 18.2-kDa hydrophobic product with five possible membrane-spanning helices, which could function as a transporter.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Henriques AO,Bryan EM,Beall BW,Moran CP Jr

doi

10.1128/jb.179.2.389-398.1997

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

389-98

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

杂志文章
  • A dominant-negative fur mutation in Bradyrhizobium japonicum.

    abstract::In many bacteria, the ferric uptake regulator (Fur) protein plays a central role in the regulation of iron uptake genes. Because iron figures prominently in the agriculturally important symbiosis between soybean and its nitrogen-fixing endosymbiont Bradyrhizobium japonicum, we wanted to assess the role of Fur in the i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.5.1409-1414.2004

    authors: Benson HP,LeVier K,Guerinot ML

    更新日期:2004-03-01 00:00:00

  • The Yersinia Yop virulon: LcrV is required for extrusion of the translocators YopB and YopD.

    abstract::LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.5.1207-1214.1998

    authors: Sarker MR,Neyt C,Stainier I,Cornelis GR

    更新日期:1998-03-01 00:00:00

  • Chemical studies on cellular components of Bordetella pertussis. I. Purification and properties of agglutinogen.

    abstract::Onoue, Kaoru, (Kyushu University, Fukuoka, Japan), Masayasu Kitagawa, and Yuichi Yamamura. Chemical studies on cellular components of Bordetella pertussis. I. Purification and properties of agglutinogen. J. Bacteriol. 82:648-656. 1961.-A method is described for the preparation of the agglutinogen of Hemophilus pertuss...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.648-656.1961

    authors: ONOUE K,KITAGAWA M,YAMAMURA Y

    更新日期:1961-11-01 00:00:00

  • Large intergenic cruciform-like supermotifs in the Lactobacillus plantarum genome.

    abstract::Twenty-four Lactobacillus plantarum supermotifs (LPSMs) with lengths from approximately 800 to 1,000 nucleotides were identified in the L. plantarum genome. LPSMs were conserved in other L. plantarum strains but not in other species. Secondary structure analysis predicted that LPSMs may fold into cruciform-like struct...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01672-08

    authors: Wels M,Bongers RS,Boekhorst J,Molenaar D,Sturme M,de Vos WM,Siezen RJ,Kleerebezem M

    更新日期:2009-05-01 00:00:00

  • Toxicity of Pasteurella tularensis killed by ionizing radiation.

    abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Landay ME,Wright GG,Pulliam JD,Finegold MJ

    更新日期:1968-09-01 00:00:00

  • SigmaS controls multiple pathways associated with intracellular multiplication of Legionella pneumophila.

    abstract::Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01578-08

    authors: Hovel-Miner G,Pampou S,Faucher SP,Clarke M,Morozova I,Morozov P,Russo JJ,Shuman HA,Kalachikov S

    更新日期:2009-04-01 00:00:00

  • Delayed expression of in vivo restriction activity following conjugal transfer of Escherichia coli hsdK (restriction-modification) genes.

    abstract::Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4905-4906.1993

    authors: Prakash-Cheng A,Ryu J

    更新日期:1993-08-01 00:00:00

  • Relationship between sulfadiazine resistance and the failure to ferment maltose in Neisseria meningitidis.

    abstract::The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.3.557-561.1967

    authors: Kingsbury DT

    更新日期:1967-09-01 00:00:00

  • Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes for the propanediol dehydratase.

    abstract::The propanediol utilization (pdu) operon of Salmonella typhimurium encodes proteins required for the catabolism of propanediol, including a coenzyme B12-dependent propanediol dehydratase. A clone that expresses propanediol dehydratase activity was isolated from a Salmonella genomic library. DNA sequence analysis showe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6633-6639.1997

    authors: Bobik TA,Xu Y,Jeter RM,Otto KE,Roth JR

    更新日期:1997-11-01 00:00:00

  • Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.

    abstract::Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling stu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.13.4399-4402.1997

    authors: Webb E,Claas K,Downs DM

    更新日期:1997-07-01 00:00:00

  • Genetic evidence of distinct physiological regulation mechanisms in the sigma(54) Pu promoter of Pseudomonas putida.

    abstract::The activity of the toluene-responsive sigma(54) Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when ame...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.4.956-960.2000

    authors: Cases I,de Lorenzo V

    更新日期:2000-02-01 00:00:00

  • Identification of mycobacterium tuberculosis DNA sequences encoding exported proteins by using phoA gene fusions.

    abstract::The activity of bacterial alkaline phosphatase (PhoA) is dependent on it being exported across the plasma membrane. A plasmid vector (pJEM11) allowing fusions between phoA and genes encoding exported proteins was constructed to study protein export in mycobacteria. Introduction of the Mycobacterium fortuitum beta-lact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.1.59-65.1995

    authors: Lim EM,Rauzier J,Timm J,Torrea G,Murray A,Gicquel B,Portnoi D

    更新日期:1995-01-01 00:00:00

  • Mechanism of action of the antifungal antibiotic pyrrolnitrin.

    abstract::Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.310-318.1969

    authors: Tripathi RK,Gottlieb D

    更新日期:1969-10-01 00:00:00

  • Characteristics of some multiply recombination-deficient strains of Escherichia coli.

    abstract::Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13. All multiple rec(-) strains carrying recA13 were similar to AB...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.231-239.1969

    authors: Willetts NS,Clark AJ

    更新日期:1969-10-01 00:00:00

  • The frequency of persisters in Escherichia coli reflects the kinetics of awakening from dormancy.

    abstract::A genetically homogenous bacterial population may contain physiologically distinct subpopulations. In one such case, a minor part of an otherwise antibiotic-sensitive bacterial population maintains a nondividing state even in a growth-supporting environment and is therefore not killed by bactericidal antibiotics. This...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00056-10

    authors: Jõers A,Kaldalu N,Tenson T

    更新日期:2010-07-01 00:00:00

  • Characterization of lipoprotein EnvA in Chlamydia psittaci 6BC.

    abstract::The primary sequence of the small cysteine-rich protein (EnvA) of Chlamydia psittaci 6BC has been shown to possess a potential lipid modification/signal peptidase II-processing site, and the mature protein was labeled by a [3H]palmitic acid precursor. We further characterized the mature EnvA, showing that it lacks the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.19.6082-6087.1994

    authors: Everett KD,Desiderio DM,Hatch TP

    更新日期:1994-10-01 00:00:00

  • Isolation and characterization of an Escherichia coli mutant affected in the regulation of adenylate cyclase.

    abstract::A mutant, cyaR1, affecting regulation of adenylate cyclase expression or activity is described. It was obtained as a thermoresistant revertant of a strain harboring a thermosensitive transcription termination factor, rho (rho-15). This mutant failed to synthesize adenosine 3',5'-phosphate and exhibited a carbohydrate-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.148.3.753-761.1981

    authors: Guidi-Rontani C,Danchin A,Ullmann A

    更新日期:1981-12-01 00:00:00

  • Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci.

    abstract::Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.2.169-180.1961

    authors: FIRSHEIN W

    更新日期:1961-08-01 00:00:00

  • Rejection of impassable substrates by Yersinia type III secretion machines.

    abstract::Type III machines of pathogenic Yersinia spp. transport Yop proteins across the bacterial envelope into host cells. Translational fusions of yopE to the dihydrofolate reductase gene (dhfr) or the beta-galactosidase gene (lacZ) generate hybrid proteins that block type III injection of Yop proteins into host cells, cons...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.20.7090-7102.2005

    authors: Sorg JA,Miller NC,Marketon MM,Schneewind O

    更新日期:2005-10-01 00:00:00

  • mRNA composition and control of bacterial gene expression.

    abstract::The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.11.3037-3044.2000

    authors: Liang ST,Xu YC,Dennis P,Bremer H

    更新日期:2000-06-01 00:00:00

  • Events following prophage Mu induction.

    abstract::Escherichia coli strains lysogenic for a thermoinducible Mu prophage (Mu cts62) undergo rapid lysis about 50 min after heat induction. Induction of Mu cts62 apparently causes damage to the host sequences in which Mu is inserted. The normal expression of A, BU, and X genes of Mu is needed for this specific deleterious ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.2.437-442.1975

    authors: Razzaki T,Bukhari AI

    更新日期:1975-05-01 00:00:00

  • Intergeneric communication in dental plaque biofilms.

    abstract::Dental plaque is a complex biofilm that accretes in a series of discrete steps proceeding from a gram-positive streptococcus-rich biofilm to a structure rich in gram-negative anaerobes. This study investigated information flow between two unrelated plaque bacteria, Streptococcus cristatus and Porphyromonas gingivalis....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.24.7067-7069.2000

    authors: Xie H,Cook GS,Costerton JW,Bruce G,Rose TM,Lamont RJ

    更新日期:2000-12-01 00:00:00

  • The response of a Bacillus subtilis temperature-sensitive sigA mutant to heat stress.

    abstract::The mutant sigA allele of Bacillus subtilis DB1005 was confirmed to be temperature sensitive (ts) and transferable among strains of B. subtilis by chromosomal transformation and gene conversion. This ts sigA allele had a pleiotropic effect on gene expression of DB1005. The induction of certain heat shock proteins in D...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3102-3110.1994

    authors: Chang BY,Chen KY,Wen YD,Liao CT

    更新日期:1994-06-01 00:00:00

  • Analysis of a mutation affecting the specificity domain for prohead binding of the bacteriophage lambda terminase.

    abstract::Genetic studies have identified a specificity domain for prohead binding in the C-terminal 32 amino acids of gpA, the large subunit of bacteriophage lambda terminase (S. Frackman, D. A. Siegele, and M. Feiss, J. Mol. Biol. 180:283-300, 1984). In the present work, an amber mutation, Aam42, in the fifth-to-last codon of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.3.850-856.1992

    authors: Sippy J,Feiss M

    更新日期:1992-02-01 00:00:00

  • Identification of proteins of the outer (L and P) rings of the flagellar basal body of Escherichia coli.

    abstract::Synthesis of the Salmonella typhimurium hook protein from the gene cloned on a multicopy plasmid results in partial suppression of the flagellar assembly defects of certain classes of Escherichia coli mutants (K. Ohnishi, M. Homma, K. Kutsukake, and T. Iino, J. Bacteriol, 169:1485-1488, 1987). This phenomenon allowed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.4.1489-1492.1987

    authors: Jones CJ,Homma M,Macnab RM

    更新日期:1987-04-01 00:00:00

  • Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis.

    abstract::Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.782-796.1985

    authors: Shaw JH,Clewell DB

    更新日期:1985-11-01 00:00:00

  • Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis.

    abstract::The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.12.4071-4074.1997

    authors: Trinh S,Reysset G

    更新日期:1997-06-01 00:00:00

  • Transduction in Bacillus subtilis.

    abstract::Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.106-111.1962

    authors: THORNE CB

    更新日期:1962-01-01 00:00:00

  • Functional characterization of a novel xylanase from a corn strain of Erwinia chrysanthemi.

    abstract::A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.2093-2100.2001

    authors: Hurlbert JC,Preston JF 3rd

    更新日期:2001-03-01 00:00:00

  • Cell wall of Mycobacterium lepraemurium strain Hawaii.

    abstract::The chemical properties of the cell wall of Mycobacterium lepraemurium strain Hawaii were investigated. Five subunits of the cell wall, arabinose mycolate, mycolic acids, tetrapeptide (Ala-Gln-diaminopimelic acid-Ala), disaccharide (N-acetylglucosaminyl-beta-1,4-N-glycolylmuramic acid), and arabinogalactan, were obtai...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.515-518.1973

    authors: Azuma I,Yamamura Y,Tanaka Y,Kosaka K,Mori T

    更新日期:1973-01-01 00:00:00