Abstract:
:Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analysis suggested that an inositol-inducible promoter for the iol operon (iol promoter) might be a promoter-like sequence in the 5' region of iolA, which is probably recognized by sigmaA. S1 nuclease analysis implied that a rho-independent terminator-like structure in the 3' region of iolJ might be a terminator for iol transcription. Disruption of the iol promoter prevented synthesis of the iol transcript as well as that of Idh, implying that the iol operon is most probably transcribed as an 11.5-kb mRNA containing the 10 iol genes. Immediately upstream of the iol operon, two genes (iolR and iolS) with divergent orientations to the iol operon were found. Disruption of iolR (but not iolS) caused constitutive synthesis of the iol transcript and Idh, indicating that the iolR gene encodes a transcription-negative regulator (presumably a repressor) for the iol operon. Northern and S1 nuclease analyses revealed that the iolRS genes were cotranscribed from another inositol-inducible promoter, which is probably recognized by sigmaA. The promoter assignments of the iol and iolRS operons were confirmed in vivo with a lacZ fusion integrated into the amyE locus.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Yoshida KI,Aoyama D,Ishio I,Shibayama T,Fujita Ydoi
10.1128/jb.179.14.4591-4598.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
4591-8issue
14eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract::After UV irradiation, Escherichia coli uvrA mutant cells show higher survival on minimal than on rich growth medium, i.e., they show minimal-medium recovery. This effect of rich growth medium on survival is not observed in a uvrA mutant carrying an mmrA1 mutation, and the uvrA mmrA strain showed the same survival rate...
journal_title:Journal of bacteriology
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doi:10.1128/JB.154.2.743-747.1983
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doi:10.1128/JB.91.6.2146-2154.1966
更新日期:1966-06-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.180.3.647-654.1998
更新日期:1998-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2006-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.901-907.1977
更新日期:1977-02-01 00:00:00
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更新日期:2007-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.171.10.5322-5324.1989
更新日期:1989-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.236-244.1966
更新日期:1966-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.560-564.1979
更新日期:1979-08-01 00:00:00
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更新日期:2004-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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doi:10.1128/JB.144.1.432-434.1980
更新日期:1980-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.965-967.1984
更新日期:1984-03-01 00:00:00
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更新日期:1999-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.8.4610-4615.1990
更新日期:1990-08-01 00:00:00
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更新日期:2003-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1320-1326.1967
更新日期:1967-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00