Role of lithium ions in proline transport in Escherichia coli.

Abstract:

:Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depending on the driving force provided. When proline was added to unbuffered cell suspensions under anaerobic conditions, the medium was found to be acidified only in the presence of Li+ but not in the presence of Na+ or K+. This acidification was abolished by the addition of a permeant anion, SCN-, to the medium containing Li+, but this was not demonstrated with cells of a mutant strain deficient in a carrier protein specific for proline. These results support the assumption that proline is taken up by a mechanism of Li+-proline cotransport in E. coli.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kayama-Gonda Y,Kawasaki T

doi

10.1128/JB.139.2.560-564.1979

subject

Has Abstract

pub_date

1979-08-01 00:00:00

pages

560-4

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

139

pub_type

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