Selection for citrate synthase deficiency in icd mutants of Escherichia coli.

Abstract:

:Cultures of isocitrate dehydrogenase-deficient (icd) mutants were overgrown by double mutants (icd glt) lacking citrate synthase activity also. The icd mutants grew more slowly than wild-type cells or the double mutants because they accumulated an inhibitory metabolite (possibly citrate). Intracellular citrate levels were several hundred-fold higher in icd cells than in wild-type or icd glt cells. Final growth yields of the wild type and the icd mutant on limiting glucose were equivalent and greater than the growth yield of icd glt double mutants. The icd gene mapped between 60 and 74 min. icd mutants were resistant to nalidixic acid, but glt and icd glt mutants and wild-type cells were sensitive, indicating that resistance results from accumulation of isocitrate, citrate, or a derivative of these compounds.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lakshmi TM,Helling RB

doi

10.1128/JB.127.1.76-83.1976

subject

Has Abstract

pub_date

1976-07-01 00:00:00

pages

76-83

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

127

pub_type

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