Transport of nonmetabolizable opines by Agrobacterium tumefaciens.

Abstract:

:We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopaline) present in the bacterium. The transport of these opines required active cellular metabolism. Nonradioactive octopine, nopaline, and arginine competed effectively with [14C]octopine and [14C]nopaline for transport into A. tumefaciens A136, suggesting that the transport of these opines occurs via an arginine transport pathway not encoded by the Ti plasmid.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Krishnan M,Burgner JW,Chilton WS,Gelvin SB

doi

10.1128/jb.173.2.903-905.1991

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

903-5

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

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