Specialized transduction with lambda plac5: dependence on recB.

abstract：:We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...

journal_title：Journal of bacteriology

authors： Strohmaier H,Remler P,Renner W,Högenauer G

更新日期：1995-08-01 00:00:00

abstract：:Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

journal_title：Journal of bacteriology

authors： McCullough J,Herskowitz I

更新日期：1979-04-01 00:00:00

abstract：:The plasmids R1162 and pSC101 have origins of conjugative transfer (oriTs) and corresponding relaxases that are closely related. The oriTs are made up of a highly conserved core, where DNA is cleaved by the relaxase prior to transfer, and an inverted repeat that differs in size and sequence. We show that in each case ...

journal_title：Journal of bacteriology

authors： Jandle S,Meyer R

更新日期：2006-01-01 00:00:00

abstract：:Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in Escherichia coli. J. Bacteriol. 91:1298-1304. 1966.-The drug-resistance factor, R, and the sex factor, F, have homologous traits, ...

journal_title：Journal of bacteriology

authors： Hirota Y,Fujii T,Nishimua Y

更新日期：1966-03-01 00:00:00

abstract：:A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

journal_title：Journal of bacteriology

authors： Hausman SZ,London J

更新日期：1987-04-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...

journal_title：Journal of bacteriology

authors： Daniel JW,Babcock K

更新日期：1966-10-01 00:00:00

abstract：:NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae UPM791, the nifA gene is part of a gene cluster (orf71 orf79 fixW orf5 fixABCX nifAB) separated by 896 bp from an upstream and divergent truncated duplication of nifH (DeltanifH). Sy...

journal_title：Journal of bacteriology

authors： Martínez M,Palacios JM,Imperial J,Ruiz-Argüeso T

更新日期：2004-10-01 00:00:00

abstract：:The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and crystallized. A temperat...

journal_title：Journal of bacteriology

authors： Dallas WS,Gowen JE,Ray PH,Cox MJ,Dev IK

更新日期：1992-09-01 00:00:00

abstract：:Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth. The role of autolysis during gonococcal infection is not known, but possible advantages for the bacterial population include provision of nutrients to a starving population, modulation of the host immune response by rele...

journal_title：Journal of bacteriology

authors： Garcia DL,Dillard JP

更新日期：2006-10-01 00:00:00

abstract：:The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extra...

journal_title：Journal of bacteriology

authors： Van Etten JL,Brambl RM

更新日期：1968-10-01 00:00:00

abstract：:Spirillum lipoferum grows vigorously on malate, succinate, lactate, or pyruvate, moderately on galactose or acetate, and poorly on glucose or citrate. It reduces 15N2. Acetylene reduction rates decrease rapidly when the pH of the culture rises above 7.8. The organism is highly aerobic and had doubling times as low as ...

journal_title：Journal of bacteriology

authors： Okon Y,Albrecht SL,Burris RH

更新日期：1976-09-01 00:00:00

abstract：:Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

journal_title：Journal of bacteriology

authors： Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

更新日期：2002-07-01 00:00:00

abstract：:Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...

journal_title：Journal of bacteriology

authors： Chapman LF,Nester EW

更新日期：1969-03-01 00:00:00

abstract：:The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...

journal_title：Journal of bacteriology

authors： Kennedy JR,Richardson SH

更新日期：1969-12-01 00:00:00

abstract：:Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...

journal_title：Journal of bacteriology

authors： Zheng G,Yan LZ,Vederas JC,Zuber P

更新日期：1999-12-01 00:00:00

abstract：:Stable, metabolically active protoplasts of Staphylococcus aureus have been prepared by the use of a staphylolytic enzyme produced by Aeromonas hydrophila. Respiratory and glycolytic rates and synthesis of nucleic acids, protein, and lipid in these protoplasts, stabilized variously in 1.1 M sucrose, 0.37 M sodium succ...

journal_title：Journal of bacteriology

authors： Coles NW,Gross R

更新日期：1973-09-01 00:00:00

abstract：:Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

journal_title：Journal of bacteriology

authors： Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

更新日期：2007-05-01 00:00:00

abstract：:Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...

journal_title：Journal of bacteriology

authors： Joshi AC,Kaur P,Nair RK,Lele DS,Nandicoori VK,Gopal B

更新日期：2019-02-25 00:00:00

abstract：:One of the pleiotropic phenotypes of mutations affecting DNA gyrase activity in Salmonella typhimurium is the constitutive deattenuation of the histidine operon. In the present work, we isolated and characterized a suppressor mutation which restores his attenuation in the presence of a defective gyrase. Such a suppres...

journal_title：Journal of bacteriology

authors： Blanc-Potard AB,Bossi L

更新日期：1994-04-01 00:00:00

abstract：:ermC specifies an rRNA methyltransferase that confers resistance to erythromycin. The expression of this determinant is induced by the addition of erythromycin. The induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. We now show that synthesis of the ermC gene pr...

journal_title：Journal of bacteriology

authors： Denoya CD,Bechhofer DH,Dubnau D

更新日期：1986-12-01 00:00:00

abstract：:Aerobacter cloacae UW-C83 and Escherichia coli K-12 were grown under various anaerobic environments. Y(atp) values were calculated by determination of cell weights and analyses for fermentation products. These Y(atp) values are compared with others reported in the literature. Limitation of growth by factors other than...

journal_title：Journal of bacteriology

authors： Hernandez E,Johnson MJ

更新日期：1967-10-01 00:00:00

abstract：:Hook-associated proteins (HAPs) were excreted into the culture medium of the Fla+ strain as well as into the growth medium of the filamentless mutants of Salmonella typhimurium. This indicates that the bacteria synthesize HAPs excessively, beyond the amount required for construction of flagella. The extra HAPs are she...

journal_title：Journal of bacteriology

authors： Homma M,Iino T

更新日期：1985-12-01 00:00:00

abstract：:Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...

journal_title：Journal of bacteriology

authors： Kanipes MI,Papp-Szabo E,Guerry P,Monteiro MA

更新日期：2006-05-01 00:00:00

abstract：:Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms. The E. coli che region was introduced into Salmonella recipients by means of a series of F-prime e...

journal_title：Journal of bacteriology

authors： DeFranco AL,Parkinson JS,Koshland DE Jr

更新日期：1979-07-01 00:00:00

abstract：:Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

journal_title：Journal of bacteriology

authors： Whelan WL,Ballou CE

更新日期：1975-12-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts. Previous work showed that the group 2 sigma factor sigE (alr4249; previously called sigF) is upregulated in differentiating heterocysts 16 h after nitrogen step-down. We now show th...

journal_title：Journal of bacteriology

authors： Mella-Herrera RA,Neunuebel MR,Kumar K,Saha SK,Golden JW

更新日期：2011-04-01 00:00:00

abstract：:Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...

journal_title：Journal of bacteriology

authors： Buckner CM,Schyns G,Moran CP Jr

更新日期：1998-07-01 00:00:00

abstract：:Transcriptional regulation was demonstrated in Rickettsia prowazekii, an obligate intracytoplasmic bacterium. The level of citrate synthase (gltA) mRNA II, from promoter P2, was greater in the total RNA isolated from heavily infected L929 cells than in moderately infected L929 cells; conversely, the level of ATP/ADP t...

journal_title：Journal of bacteriology

authors： Cai J,Winkler HH

更新日期：1996-09-01 00:00:00

abstract：:We report a draft sequence of the genome of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (Neofelis nebulosa). As predicted, the reported genome contains several gene clusters for cholesterol degradation. This is the second available genome seq...

journal_title：Journal of bacteriology

authors： Ge F,Li W,Chen G,Liu Y,Zhang G,Yong B,Wang Q,Wang N,Huang Z,Li W,Wang J,Wu C,Xie Q,Liu G

更新日期：2011-09-01 00:00:00

abstract：:The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highl...

journal_title：Journal of bacteriology

authors： Vonder Haar RA,Legrain M,Kolbe HV,Jacobs E

更新日期：1994-10-01 00:00:00