Levels and activity of the Pseudomonas putida global regulatory protein Crc vary according to growth conditions.

abstract：:ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...

journal_title：Journal of bacteriology

authors： Berlyn MB,Giles NH

更新日期：1969-07-01 00:00:00

abstract：:The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...

journal_title：Journal of bacteriology

authors： Osman SF,Fett WF

更新日期：1989-03-01 00:00:00

abstract：:Two unusual characteristics of some outer membrane proteins of Rhizobium leguminosarum are described. First, most of the major outer membrane proteins could only be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after lysozyme treatment of the isolated cell envelopes, suggesting a very strong,...

journal_title：Journal of bacteriology

authors： de Maagd RA,Wientjes FB,Lugtenberg BJ

更新日期：1989-07-01 00:00:00

abstract：:The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...

journal_title：Journal of bacteriology

authors： Chatfield LK,Orr E,Boulnois GJ,Wilkins BM

更新日期：1982-12-01 00:00:00

abstract：:The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...

journal_title：Journal of bacteriology

authors： Every D,Skerman TM

更新日期：1980-02-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:In the chloroplasts of higher plants and algae, the biosynthesis of the chlorophyll precursor delta-aminolevulinic acid (ALA) involves at least three enzymes and a tRNA species. Here we demonstrate that in cell extracts of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 ALA was formed from glutamate i...

journal_title：Journal of bacteriology

authors： O'Neill GP,Peterson DM,Schön A,Chen MW,Söll D

更新日期：1988-09-01 00:00:00

abstract：:The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...

journal_title：Journal of bacteriology

authors： Trinh S,Reysset G

更新日期：1997-06-01 00:00:00

abstract：:In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254. ...

journal_title：Journal of bacteriology

authors： Kunnimalaiyaan S,Rakowski SA,Filutowicz M

更新日期：2007-07-01 00:00:00

abstract：:The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gen...

journal_title：Journal of bacteriology

authors： Metcalf WW,Zhang JK,Shi X,Wolfe RS

更新日期：1996-10-01 00:00:00

abstract：:Although the pervasive soil and water microorganism Pseudomonas aeruginosa demonstrates heightened sensitivity to UV radiation, this species possesses a recA gene that, based on structural and functional properties, could mediate a DNA damage-responsive regulon similar to the SOS regulon of Escherichia coli. To determ...

journal_title：Journal of bacteriology

authors： Warner-Bartnicki AL,Miller RV

更新日期：1992-03-01 00:00:00

abstract：:Cyanothece sp. strain ATCC 51142 is a unicellular, diazotrophic cyanobacterium which demonstrated extensive metabolic periodicities of photosynthesis, respiration, and nitrogen fixation when grown under N2-fixing conditions. N2 fixation and respiration peaked at 24-h intervals early in the dark or subjective-dark peri...

journal_title：Journal of bacteriology

authors： Colón-López MS,Sherman DM,Sherman LA

更新日期：1997-07-01 00:00:00

abstract：:The function of serine protease EpiP in epidermin biosynthesis was investigated. Epidermin is synthesized as a 52-amino-acid precursor peptide, EpiA, which is posttranslationally modified and processed to the mature 22-amino-acid peptide antibiotic. epiP was expressed in Staphylococcus carnosus with xylose-regulated e...

journal_title：Journal of bacteriology

authors： Geissler S,Götz F,Kupke T

更新日期：1996-01-01 00:00:00

abstract：:In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...

journal_title：Journal of bacteriology

authors： Pianzzola MJ,Soubes M,Touati D

更新日期：1996-12-01 00:00:00

abstract：:In this study, we show that Salmonella produces an O-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. Structural analysis of purified Salmonella extracellular polysaccharides yielded predominantly a repeating oligosaccharide unit similar to that of Salmonella enterica serova...

journal_title：Journal of bacteriology

authors： Gibson DL,White AP,Snyder SD,Martin S,Heiss C,Azadi P,Surette M,Kay WW

更新日期：2006-11-01 00:00:00

abstract：:The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10 degrees C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstab...

journal_title：Journal of bacteriology

authors： Tanabe H,Goldstein J,Yang M,Inouye M

更新日期：1992-06-01 00:00:00

abstract：:Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...

journal_title：Journal of bacteriology

authors： Higashi T,Iwasaki Y,Ohnishi Y,Horinouchi S

更新日期：2007-05-01 00:00:00

abstract：:Cnm is a surface-associated protein present in a subset of Streptococcus mutans strains that mediates binding to extracellular matrices, intracellular invasion, and virulence. Here, we showed that cnm transcription is controlled by the global regulators CovR and VicRKX. In silico analysis identified multiple putative ...

journal_title：Journal of bacteriology

authors： Araújo Alves L,Ganguly T,Mattos-Graner RO,Kajfasz J,Harth-Chu EN,Lemos JA,Abranches J

更新日期：2018-11-06 00:00:00

abstract：:A number of laboratories are now engaged in the genetic analysis of Candida albicans. This diploid yeast, the major fungal pathogen of humans, is imperfect. Parasexual techniques have been devised for complementation and recombination analysis in this organism. This paper attempts to address the question of the extent...

journal_title：Journal of bacteriology

authors： Poulter R,Hanrahan V

更新日期：1983-11-01 00:00:00

abstract：:Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells...

journal_title：Journal of bacteriology

authors： Kay WW,Phipps BM,Ishiguro EE,Trust TJ

更新日期：1985-12-01 00:00:00

abstract：:pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...

journal_title：Journal of bacteriology

authors： Cornelis G,Bennett PM,Grinsted J

更新日期：1976-09-01 00:00:00

abstract：:An R plasmid, R100-1, was mapped previously (Yoshikawa, 1974) by transduction from an integratively suppressed Hfr strain to a recipient with a mutation in gene dnaA. By this method various types of transductants of plasmid R100-1 that exist autonomously or in the integrated state were obtained. Seventy-one such trans...

journal_title：Journal of bacteriology

authors： Yoshikawa M

更新日期：1975-12-01 00:00:00

abstract：:An S-adenosylhomocysteine deaminase has been isolated and purified from streptonigrin-producing Streptomyces flocculus ATCC 13257. Deamination represents the major metabolic route of S-adenosylhomocysteine in this organism. The protein was found to be monomeric with a molecular weight of 56,100 +/- 1,600. The activity...

journal_title：Journal of bacteriology

authors： Zulty JJ,Speedie MK

更新日期：1989-12-01 00:00:00

abstract：:Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...

journal_title：Journal of bacteriology

authors： Xu D,Yang C,Kaplan HB

更新日期：1998-12-01 00:00:00

abstract：:Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...

journal_title：Journal of bacteriology

authors： Helmstetter CE

更新日期：1974-10-01 00:00:00

abstract：:Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...

journal_title：Journal of bacteriology

authors： Vinuesa P,Reuhs BL,Breton C,Werner D

更新日期：1999-09-01 00:00:00

abstract：:Citrate lyase, the key enzyme of anaerobic citrate catabolism, could not be deleted from Salmonella typhimurium. The only class of mutants found had a mode of covalent regulation that strongly resembled the Escherichia coli system: citrate lyase was only active, i.e., acetylated, when a cosubstrate was present. ...

journal_title：Journal of bacteriology

authors： Kulla HG

更新日期：1983-01-01 00:00:00

abstract：:Colicinogenic factors ColI and ColV, which have been shown to behave as sex factors, could not be induced with mitomycin C. In contrast, the ColE(1), ColE(2), and ColE(3) factors, which do not exhibit any fertility factor characteristics, are inducible by this agent. The induced production of colicins E(1), E(2), and ...

journal_title：Journal of bacteriology

authors： Herschman HR,Helinski DR

更新日期：1967-09-01 00:00:00

abstract：:In the thermophilic archaeon Methanobacterium thermoautotrophicum Marburg, the structural gene for isoleucyl-tRNA synthetase (ileS) is flanked upstream by orf401 and downstream by purL. orf401 encodes a 43.5-kDa protein with an unknown function. Northern (RNA) hybridization and S1 nuclease protection experiments showe...

journal_title：Journal of bacteriology

authors： Jenal U,Thurner C,Leisinger T

更新日期：1993-09-01 00:00:00

abstract：:Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13. All multiple rec(-) strains carrying recA13 were similar to AB...

journal_title：Journal of bacteriology

authors： Willetts NS,Clark AJ

更新日期：1969-10-01 00:00:00