Regulatory citrate lyase mutants of Salmonella typhimurium.

abstract：:The secondary metabolite hydrogen cyanide (HCN) is produced by Pseudomonas fluorescens from glycine, essentially under microaerophilic conditions. The genetic basis of HCN synthesis in P. fluorescens CHA0 was investigated. The contiguous structural genes hcnABC encoding HCN synthase were expressed from the T7 promoter...

journal_title：Journal of bacteriology

authors： Laville J,Blumer C,Von Schroetter C,Gaia V,Défago G,Keel C,Haas D

更新日期：1998-06-01 00:00:00

abstract：:LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...

journal_title：Journal of bacteriology

authors： Bowers CW,Lau F,Silhavy TJ

更新日期：2003-10-01 00:00:00

abstract：:Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...

journal_title：Journal of bacteriology

authors： Loewen PC,Switala J

更新日期：1987-12-01 00:00:00

abstract：:Cultures of recombination-deficient strains of Escherichia coli are composed of three classes of cells: (i) viable cells, which can undergo 20 or more generations, (ii) residually dividing cells, which can undergo fewer than 20 generations (probably an average of fewer than 6), and (iii) nondividing cells, which are i...

journal_title：Journal of bacteriology

authors： Capaldo FN,Ramsey G,Barbour SD

更新日期：1974-04-01 00:00:00

abstract：:Proteus mirabilis, an agent of urinary tract infection, expresses at least four fimbrial types. Among these are the MR/P (mannose-resistant/Proteus-like) fimbriae. MrpA, the structural subunit, is optimally expressed at 37 degrees C in Luria broth cultured statically for 48 h by each of seven strains examined. Genes e...

journal_title：Journal of bacteriology

authors： Bahrani FK,Mobley HL

更新日期：1994-06-01 00:00:00

abstract：:The Escherichia coli gene cluster encoding enzymatic activities responsible for the synthesis and activation of 2,3-dihydroxybenzoic acid in the formation of the catechol siderophore enterobactin was localized to a 4.2-kilobase chromosomal DNA fragment. Analysis of various subclones and transposon insertion mutations ...

journal_title：Journal of bacteriology

authors： Nahlik MS,Fleming TP,McIntosh MA

更新日期：1987-09-01 00:00:00

abstract：:The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times...

journal_title：Journal of bacteriology

authors： Willison JC,Tissot G

更新日期：1994-06-01 00:00:00

abstract：:ermC specifies an rRNA methyltransferase that confers resistance to erythromycin. The expression of this determinant is induced by the addition of erythromycin. The induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. We now show that synthesis of the ermC gene pr...

journal_title：Journal of bacteriology

authors： Denoya CD,Bechhofer DH,Dubnau D

更新日期：1986-12-01 00:00:00

abstract：:A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

journal_title：Journal of bacteriology

authors： Rastogi VK,Watson RJ

更新日期：1991-05-01 00:00:00

abstract：:Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...

journal_title：Journal of bacteriology

authors： Serrano M,Vieira F,Moran CP Jr,Henriques AO

更新日期：2008-12-01 00:00:00

abstract：:Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

journal_title：Journal of bacteriology

authors： Sakib SN,Reddi G,Almagro-Moreno S

更新日期：2018-03-26 00:00:00

abstract：:The Escherichia coli pap genetic determinant includes 11 genes and encodes expression of Pap pili on the bacterial surface. An RNase E-dependent mRNA-processing event in the intercistronic papB-papA region results in the accumulation of a papA-gene-specific mRNA in considerable excess of the primary papB-papA mRNA tra...

journal_title：Journal of bacteriology

authors： Nilsson P,Naureckiene S,Uhlin BE

更新日期：1996-02-01 00:00:00

abstract：:Most strains of Pseudomonas aeruginosa can express two chemically and immunologically distinct types of lipopolysaccharide (LPS), an antigenically conserved form called A band and the serotype-specific form called B band. To study the molecular controls regulating expression of the A-band LPS antigen, we have cloned t...

journal_title：Journal of bacteriology

authors： Lightfoot J,Lam JS

更新日期：1991-09-01 00:00:00

abstract：:Studies on the composition of total fatty acids of Alcaligenes faecalis harvested at different growth phases have been carried out. Ability of the organism to desaturate palmitic and stearic acid has also been tested. The organism contained palmitic (16:0), stearic (18:0), palmitoleic (16:1), cis-vaccenic (18:1), cycl...

journal_title：Journal of bacteriology

authors： Ghaneker AS,Nair PM

更新日期：1973-05-01 00:00:00

abstract：:The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR...

journal_title：Journal of bacteriology

authors： Ramon AM,Porta A,Fonzi WA

更新日期：1999-12-01 00:00:00

abstract：:Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

journal_title：Journal of bacteriology

authors： Caparrós M,Pisabarro AG,de Pedro MA

更新日期：1992-09-01 00:00:00

abstract：:The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...

journal_title：Journal of bacteriology

authors： Aygun-Sunar S,Bilaloglu R,Goldfine H,Daldal F

更新日期：2007-12-01 00:00:00

abstract：:Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...

journal_title：Journal of bacteriology

authors： Hitchcock MJ,Hodgson B,Linforth JL

更新日期：1980-05-01 00:00:00

abstract：:A cosmid vector has been constructed, using a lambda replicon. A library of cosmids from Anabaena variabilis ATCC 29413 based on use of this vector is shown to be highly deficient in sites for the two type II restriction endonucleases found in that organism. ...

journal_title：Journal of bacteriology

authors： Herrero A,Elhai J,Hohn B,Wolk CP

更新日期：1984-11-01 00:00:00

abstract：:An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...

journal_title：Journal of bacteriology

authors： Lackey D,Walker GC,Keng T,Linn S

更新日期：1977-08-01 00:00:00

abstract：:Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Mutations in gldN cause a partial defect in gliding. A novel bacteriophage selection strategy was used to aid construction of a strain with a deletion spanning gldN and the closely related gene gldO in an otherwise wild-type F. johnso...

journal_title：Journal of bacteriology

authors： Rhodes RG,Samarasam MN,Shrivastava A,van Baaren JM,Pochiraju S,Bollampalli S,McBride MJ

更新日期：2010-03-01 00:00:00

abstract：:We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expres...

journal_title：Journal of bacteriology

authors： Tsui HC,Mukherjee D,Ray VA,Sham LT,Feig AL,Winkler ME

更新日期：2010-01-01 00:00:00

abstract：:We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...

journal_title：Journal of bacteriology

authors： Pereira SF,Henriques AO,Pinho MG,de Lencastre H,Tomasz A

更新日期：2007-05-01 00:00:00

abstract：:The fatty acid pattern in three hydrocarbon-utilizing bacteria during growth on various substrates was examined. The predominant fatty acids in acetate-grown cells were C(16), C(16:1), C(18:1), and Br-C(19) and the major fatty acids in propane-grown cells were C(15), C(17), C(17:1), C(18:1), and Br-C(18). When one org...

journal_title：Journal of bacteriology

authors： Dunlap KR,Perry JJ

更新日期：1967-12-01 00:00:00

abstract：:The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma class of carbonic anhydrases. A search of databases queried with Mt-Cam revealed that a majority of the homologs comprise a putative subclass (CamH) in which there is major conservation of all of the residues essential for the...

journal_title：Journal of bacteriology

authors： Zimmerman SA,Tomb JF,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:The Escherichia coli lacZ gene was used as a model system to identify specific sequence elements affecting mRNA stability. Various insertions and substitutions at the ribosome-binding site increased or decreased the rate of mRNA inactivation by up to fourfold. Deletion of a dyad symmetry, which may give rise to a very...

journal_title：Journal of bacteriology

authors： Petersen C

更新日期：1991-04-01 00:00:00

abstract：:The antibiotic peptide nisin is the first known lantibiotic that uses a docking molecule within the bacterial cytoplasmic membrane for pore formation. Through specific interaction with the cell wall precursor lipid II, nisin forms defined pores which are stable for seconds and have pore diameters of 2 to 2.5 nm. ...

journal_title：Journal of bacteriology

authors： Wiedemann I,Benz R,Sahl HG

更新日期：2004-05-01 00:00:00

abstract：:The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highl...

journal_title：Journal of bacteriology

authors： Vonder Haar RA,Legrain M,Kolbe HV,Jacobs E

更新日期：1994-10-01 00:00:00

abstract：:Bacteria use three distinct systems for iron-sulfur (Fe/S) cluster biogenesis: the ISC, SUF, and NIF machineries. The ISC and SUF systems are widely distributed, and many bacteria possess both of them. In Escherichia coli, ISC is the major and constitutive system, whereas SUF is induced under iron starvation and/or ox...

journal_title：Journal of bacteriology

authors： Albrecht AG,Netz DJ,Miethke M,Pierik AJ,Burghaus O,Peuckert F,Lill R,Marahiel MA

更新日期：2010-03-01 00:00:00

abstract：:We performed a systematic survey of transcription of a large region of the Rhizobium meliloti symbiotic plasmid pSym. This led to the discovery of two new sequences induced during symbiosis. The first sequence was linked to the known nitrogen fixation (nif-fix) gene cluster, and its expression depended on the nifA gen...

journal_title：Journal of bacteriology

authors： David M,Domergue O,Pognonec P,Kahn D

更新日期：1987-05-01 00:00:00