Role of PBP1 in cell division of Staphylococcus aureus.

Abstract:

:We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methicillin-susceptible strain, each carrying the pbpA mutation, were unable to grow in the absence of the inducer. Conditional mutants of pbpA transferred into IPTG-free medium underwent a four- to fivefold increase in cell mass, which was not accompanied by a proportional increase in viable titer. Examination of thin sections of such cells by transmission electron microscopy or fluorescence microscopy of intact cells with Nile red-stained membranes showed a morphologically heterogeneous population of bacteria with abnormally increased sizes, distorted axial ratios, and a deficit in the number of cells with completed septa. Immunofluorescence with an antibody specific for PBP1 localized the protein to sites of cell division. No alteration in the composition of peptidoglycan was detectable in pbpA conditional mutants grown in the presence of a suboptimal concentration of IPTG, which severely restricted the rate of growth, and the essential function of PBP1 could not be replaced by PBP2A present in methicillin-resistant cells. These observations suggest that PBP1 is not a major contributor to the cross-linking of peptidoglycan and that its essential function must be intimately integrated into the mechanism of cell division.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pereira SF,Henriques AO,Pinho MG,de Lencastre H,Tomasz A

doi

10.1128/JB.00044-07

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

3525-31

issue

9

eissn

0021-9193

issn

1098-5530

pii

JB.00044-07

journal_volume

189

pub_type

杂志文章
  • Cell division in a species of Erwinia IV. Metabolic blocks in panothenate biosynthesis and their relationship to inhibition of cell division.

    abstract::Grula, Mary M. (Oklahoma State University, Stillwater) and E. A. Grula. Cell division in a species of Erwinia. IV. Metabolic blocks in pantothenate biosynthesis and their relationship to inhibition of cell division. J. Bacteriol. 83:989-997. 1962.-Four compounds that inhibit cell division in an Erwinia sp., d-serine, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.5.989-997.1962

    authors: GRULA MM,GRULA EA

    更新日期:1962-05-01 00:00:00

  • Genome sequence of a novel nicotine-degrading strain, Pseudomonas geniculata N1.

    abstract::A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00625-12

    authors: Tang H,Yu H,Tai C,Huang K,Liu Y,Wang L,Yao Y,Wu G,Xu P

    更新日期:2012-07-01 00:00:00

  • SecA dimer cross-linked at its subunit interface is functional for protein translocation.

    abstract::SecA facilitates protein transport across the eubacterial plasma membrane by its association with cargo proteins and the SecYEG translocon, followed by ATP-driven conformational changes that promote protein translocation in a stepwise manner. Whether SecA functions as a monomer or a dimer during this process has been ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.1.335-338.2006

    authors: Jilaveanu LB,Oliver D

    更新日期:2006-01-01 00:00:00

  • The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome.

    abstract::The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA). Compared with isogenic rep(+) strains, rep mutants show the following characteristics: larger cell size, more DNA per cell, and a slightly lower DNA/mass ratio. The replicating rep ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.805-814.1974

    authors: Lane HE,Denhardt DT

    更新日期:1974-11-01 00:00:00

  • Response of the hyperthermophilic archaeon Sulfolobus solfataricus to UV damage.

    abstract::In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01016-07

    authors: Fröls S,Gordon PM,Panlilio MA,Duggin IG,Bell SD,Sensen CW,Schleper C

    更新日期:2007-12-01 00:00:00

  • DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a phage-tail-like bacteriocin of phytopathogenic Erwinia carotovora subsp. carotovora Er.

    abstract::Carotovoricin Er is a phage-tail-like bacteriocin produced by Erwinia carotovora subsp. carotovora strain Er, a causative agent for soft rot disease in plants. Here we studied binding and killing spectra of carotovoricin Er preparations for various strains of the bacterium (strains 645Ar, EC-2, N786, and P7) and found...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.21.6274-6281.2001

    authors: Nguyen HA,Tomita T,Hirota M,Kaneko J,Hayashi T,Kamio Y

    更新日期:2001-11-01 00:00:00

  • Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase.

    abstract::By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.366-374.1977

    authors: Niemann H,Kwiatkowski B,Westphal U,Stirm S

    更新日期:1977-04-01 00:00:00

  • The glycosyltransferase domain of penicillin-binding protein 2a from Streptococcus pneumoniae catalyzes the polymerization of murein glycan chains.

    abstract::The bacterial peptidoglycan consists of glycan chains of repeating beta-1,4-linked N-acetylglucosaminyl-N-acetylmuramyl units cross-linked through short peptide chains. The polymerization of the glycans, or glycosyltransfer (GT), and transpeptidation (TP) are catalyzed by bifunctional penicillin-binding proteins (PBPs...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.15.4418-4423.2003

    authors: Di Guilmi AM,Dessen A,Dideberg O,Vernet T

    更新日期:2003-08-01 00:00:00

  • Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells.

    abstract::THREE PREVIOUSLY STUDIED R FACTORS WERE USED: 222/R4, controlling transmissible resistance to sulfonamide, streptomycin, chloromycetin, and tetracycline (SU(r) SM(r) CM(r) TC(r)); 222/R3, a derivative of 222/R4 (now termed 222/R3W) having lost TC(r); and R15, controlling infectious resistance to SU and SM only. Two ty...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.1.166-177.1970

    authors: Nisioka T,Mitani M,Clowes RC

    更新日期:1970-07-01 00:00:00

  • Cytosine methylation by the SuaI restriction-modification system: implications for genetic fidelity in a hyperthermophilic archaeon.

    abstract::5-methylcytosine in chromosomal DNA represents a potential source of frequent spontaneous mutation for hyperthermophiles. To determine the relevance of this threat for the archaeon Sulfolobus acidocaldarius, the mode of GGCC methylation by its restriction-modification system, SuaI, was investigated. Distinct isoschizo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.15.4657-4661.2003

    authors: Grogan DW

    更新日期:2003-08-01 00:00:00

  • Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli.

    abstract::Lysyl-transfer ribonucleic acid synthetase (EC 6.1.1.6) was identified as four polypeptide spots after two-dimensional polyacrylamide gel electrophoresis of whole-cell lysates of Escherichia coli. Identification was made by migration with partially purified enzyme preparations, by peptide map patterns, by mutant analy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.345-351.1981

    authors: Hirshfield IN,Bloch PL,Van Bogelen RA,Neidhardt FC

    更新日期:1981-04-01 00:00:00

  • Activity and transcriptional regulation of bacterial protein-like glycerol-3-phosphate dehydrogenase of the haloarchaea in Haloferax volcanii.

    abstract::Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00276-11

    authors: Rawls KS,Martin JH,Maupin-Furlow JA

    更新日期:2011-09-01 00:00:00

  • New Twists and Turns in Bacterial Locomotion and Signal Transduction.

    abstract::Prokaryotic organisms occupy the most diverse set of environments and conditions on our planet. Their ability to sense and respond to a broad range of external cues remain key research areas in modern microbiology, central to behaviors that underlie beneficial and pathogenic interactions of bacteria with multicellular...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00439-19

    authors: Watts KJ,Vaknin A,Fuqua C,Kazmierczak BI

    更新日期:2019-09-20 00:00:00

  • Nonrandom minichromosome replication in Escherichia coli K-12.

    abstract::The intervals between rounds of chromosome and minichromosome replication were measured by density shift experiments and found to be similar. Thus the minichromosome, a lambda asnA oriC bacteriophage, mostly replicates once each division cycle rather than randomly, despite its high copy number. Slight differences betw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.430-433.1987

    authors: Koppes LJ,von Meyenburg K

    更新日期:1987-01-01 00:00:00

  • The RpoS sigma factor in the dissimilatory Fe(III)-reducing bacterium Geobacter sulfurreducens.

    abstract::Geobacter sulfurreducens RpoS sigma factor was shown to contribute to survival in stationary phase and upon oxygen exposure. Furthermore, a mutation in rpoS decreased the rate of reduction of insoluble Fe(III) but not of soluble forms of iron. This study suggests that RpoS plays a role in regulating metabolism of Geob...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.16.5543-5546.2004

    authors: Núñez C,Adams L,Childers S,Lovley DR

    更新日期:2004-08-01 00:00:00

  • Functional characterization of SsaE, a novel chaperone protein of the type III secretion system encoded by Salmonella pathogenicity island 2.

    abstract::The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00863-09

    authors: Miki T,Shibagaki Y,Danbara H,Okada N

    更新日期:2009-11-01 00:00:00

  • Morphogenesis of the bacterial division septum: identification of potential sites of division in lkyD mutants of Salmonella typhimurium.

    abstract::It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied un...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.1019-1024.1980

    authors: Fung JC,MacAlister TJ,Weigand RA,Rothfield LI

    更新日期:1980-08-01 00:00:00

  • Amplification and deletion of the amyE+-tmrB+ gene region in a Bacillus subtilis recombinant-phage genome by the tmrA7 mutation.

    abstract::A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The amyE+-tmrB+ gene region, approximately 12.6 kilobases, in the phage genome was amplified in a tunicamycin-resistant (Tmr) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.2.549-556.1986

    authors: Furusato T,Takano J,Yamane K,Hashiguchi K,Tanimoto A,Mori M,Yoda K,Yamasaki M,Tamura G

    更新日期:1986-02-01 00:00:00

  • Genome sequence of the repetitive-sequence-rich Mycoplasma fermentans strain M64.

    abstract::Mycoplasma fermentans is a microorganism commonly found in the genitourinary and respiratory tracts of healthy individuals and AIDS patients. The complete genome of the repetitive-sequence-rich M. fermentans strain M64 is reported here. Comparative genomics analysis revealed dramatic differences in genome size between...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05228-11

    authors: Shu HW,Liu TT,Chan HI,Liu YM,Wu KM,Shu HY,Tsai SF,Hsiao KJ,Hu WS,Ng WV

    更新日期:2011-08-01 00:00:00

  • The thioredoxin system of Penicillium chrysogenum and its possible role in penicillin biosynthesis.

    abstract::Penicillium chrysogenum is an important producer of penicillin antibiotics. A key step in their biosynthesis is the oxidative cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N by the enzyme isopenicillin N synthase (IPNS). bis-ACV, the oxidized disulfide form of ACV is, however, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.4.973-984.1994

    authors: Cohen G,Argaman A,Schreiber R,Mislovati M,Aharonowitz Y

    更新日期:1994-02-01 00:00:00

  • Role of transmembrane pH gradient and membrane binding in nisin pore formation.

    abstract::Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.1.135-140.1997

    authors: Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

    更新日期:1997-01-01 00:00:00

  • Construction of an Enterococcus faecalis Tn917-mediated-gene-disruption library offers insight into Tn917 insertion patterns.

    abstract::Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.21.7280-7289.2004

    authors: Garsin DA,Urbach J,Huguet-Tapia JC,Peters JE,Ausubel FM

    更新日期:2004-11-01 00:00:00

  • Impact of phosphorylation of specific residues in the tyrosine autokinase, Wzc, on its activity in assembly of group 1 capsules in Escherichia coli.

    abstract::Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6437-6447.2002

    authors: Paiment A,Hocking J,Whitfield C

    更新日期:2002-12-01 00:00:00

  • EFFECT OF TUNGSTATE ON THE UPTAKE AND FUNCTION OF MOLYBDATE IN AZOTOBACTER AGILIS.

    abstract::Bulen, William A. (Charles F. Kettering Foundation, Yellow Springs, Ohio). Effect of tungstate on the uptake and function of molybdate in Azotobacter agilis. J. Bacteriol. 82:130-134. 1961.-The reported competitive inhibition of molybdate by tungstate was investigated in an effort to elucidate molybdenum functions ass...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.1.130-134.1961

    authors: Bulen WA

    更新日期:1961-07-01 00:00:00

  • Iron respiration-driven proton translocation in aerobic bacteria.

    abstract::Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.729-731.1986

    authors: Short KA,Blakemore RP

    更新日期:1986-08-01 00:00:00

  • Effect of degradative plasmid CAM-OCT on responses of Pseudomonas bacteria to UV light.

    abstract::The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.975-982.1989

    authors: McBeth DL

    更新日期:1989-02-01 00:00:00

  • Allosteric regulation of the glucose:H+ symporter of Lactobacillus brevis: cooperative binding of glucose and HPr(ser-P).

    abstract::Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.7.1900-1902.1995

    authors: Ye JJ,Saier MH Jr

    更新日期:1995-04-01 00:00:00

  • NONPHOTOREACTIVATING REPAIR OF ULTRAVIOLET LIGHT-DAMAGED MICROCOCCUS LYSODEIKTICUS CELLS.

    abstract::Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.5.1225-1230.1965

    authors: ELDER RL,BEERS RF Jr

    更新日期:1965-05-01 00:00:00

  • Complete genome sequence of Bifidobacterium breve CECT 7263, a strain isolated from human milk.

    abstract::Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00691-12

    authors: Jiménez E,Villar-Tajadura MA,Marín M,Fontecha J,Requena T,Arroyo R,Fernández L,Rodríguez JM

    更新日期:2012-07-01 00:00:00

  • Complementation between uncF alleles affecting assembly of the F1F0-ATPase complex of Escherichia coli.

    abstract::A mutant affected in the b subunit (coded by the uncF gene) of the F1F0-ATPase in Escherichia coli was isolated by a localized mutagenesis procedure in which a plasmid carrying the unc genes was mutagenized in vivo. The biochemical properties of cells carrying the uncF515 allele were examined in a strain carrying the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.420-426.1985

    authors: Jans DA,Hatch L,Fimmel AL,Gibson F,Cox GB

    更新日期:1985-04-01 00:00:00