Allosteric regulation of the glucose:H+ symporter of Lactobacillus brevis: cooperative binding of glucose and HPr(ser-P).

Abstract:

:Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant analog of HPr which conformationally resembles HPr(ser-P) but not free HPr, specifically binds to membranes derived from glucose-grown L. brevis cells if and only if a substrate of the glucose permease is also present.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ye JJ,Saier MH Jr

doi

10.1128/jb.177.7.1900-1902.1995

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

1900-2

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

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