Morphogenesis of the bacterial division septum: identification of potential sites of division in lkyD mutants of Salmonella typhimurium.

Abstract:

:It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied under conditions in which ingrowth of inner membrane and murein was prevented by inactivation of the envA gene product. In aseptate filaments of the LkyD EnvA strain, outer membrane blebs occurred with the usual frequency and were preferentially located over regions where new septa were formed when cell division was subsequently permitted to resume. The results indicate that the outer membrane blebs of the LkyD strain are markers for potential sites of cell division, implying that an alteration in association of outer membrane and murein exists in these sites before the initiation of septal ingrowth. This localized change in cell envelope organization is independent of the septation-inducing effects of the envA gene product.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Fung JC,MacAlister TJ,Weigand RA,Rothfield LI

doi

10.1128/JB.143.2.1019-1024.1980

subject

Has Abstract

pub_date

1980-08-01 00:00:00

pages

1019-24

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

143

pub_type

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