Characterization of the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in the initiating glycosyltransferase and polymerase steps.

abstract：:A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...

journal_title：Journal of bacteriology

authors： Bradshaw HD Jr,Traxler BA,Minkley EG Jr,Nester EW,Gordon MP

更新日期：1990-07-01 00:00:00

abstract：:We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

journal_title：Journal of bacteriology

authors： Tian H,Beckwith J

更新日期：2002-01-01 00:00:00

abstract：:The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

journal_title：Journal of bacteriology

authors： Peng JB,Yan WM,Bao XZ

更新日期：1994-05-01 00:00:00

abstract：:The plasmids R1162 and pSC101 have origins of conjugative transfer (oriTs) and corresponding relaxases that are closely related. The oriTs are made up of a highly conserved core, where DNA is cleaved by the relaxase prior to transfer, and an inverted repeat that differs in size and sequence. We show that in each case ...

journal_title：Journal of bacteriology

authors： Jandle S,Meyer R

更新日期：2006-01-01 00:00:00

abstract：:Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.). Hydrolysis of proteins and nucleic acids by Lancefield group A and other streptococci. J. Bacteriol. 86:1270-1274. 1963.-Classically, group A streptococci have been considered to be nonproteolytic in spite of the observation that a proteinase is produc...

journal_title：Journal of bacteriology

authors： DEIBEL RH

更新日期：1963-12-01 00:00:00

abstract：:SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are Sig...

journal_title：Journal of bacteriology

authors： Hümpel A,Gebhard S,Cook GM,Berney M

更新日期：2010-05-01 00:00:00

abstract：:The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...

journal_title：Journal of bacteriology

authors： Wang RJ,Morse HG,Morse ML

更新日期：1970-12-01 00:00:00

abstract：:Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily u...

journal_title：Journal of bacteriology

authors： Hill KE,Marchesi JR,Weightman AJ

更新日期：1999-04-01 00:00:00

abstract：:The autolytic capacity of Streptococcus faecium (S. faecalis ATCC 9790) varied during synchronous cell division. This phenomenon was initially observed in rapidly dividing populations (TD=30 to 33 min) synchronized by a combination of induction and size selection techniques. To minimize the problems inherent in studie...

journal_title：Journal of bacteriology

authors： Hinks RP,Daneo-Moore L,Shockman GD

更新日期：1978-02-01 00:00:00

abstract：:Phosphate import is required for the growth of mycobacteria and is regulated by environmental inorganic phosphate (P(i)) concentrations, although the mechanism of this regulation has not been characterized. The expression of genes involved in P(i) acquisition is frequently regulated by two-component regulatory systems...

journal_title：Journal of bacteriology

authors： Glover RT,Kriakov J,Garforth SJ,Baughn AD,Jacobs WR Jr

更新日期：2007-08-01 00:00:00

abstract：:Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

journal_title：Journal of bacteriology

authors： Paiment A,Hocking J,Whitfield C

更新日期：2002-12-01 00:00:00

abstract：:All Escherichia coli strains so far examined possess a chromosomally encoded nanATEK-yhcH operon for the catabolism of sialic acids. These unique nine-carbon sugars are synthesized primarily by higher eukaryotes and can be used as carbon, nitrogen, and energy sources by a variety of microbial pathogens or commensals. ...

journal_title：Journal of bacteriology

authors： Kalivoda KA,Steenbergen SM,Vimr ER,Plumbridge J

更新日期：2003-08-01 00:00:00

abstract：:The transport of the tricarboxylic acid cycle C(4)-dicarboxylic acids was studied in both the wild-type strain and tricarboxylic acid cycle mutants of Bacillus subtilis. Active transport of malate, fumarate, and succinate was found to be inducible by these dicarboxylic acids or by precursors to them, whereas glucose o...

journal_title：Journal of bacteriology

authors： Ghei OK,Kay WW

更新日期：1973-04-01 00:00:00

abstract：:The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl ...

journal_title：Journal of bacteriology

authors： Maier E,Polleichtner G,Boeck B,Schinzel R,Benz R

更新日期：2001-01-01 00:00:00

abstract：:The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

journal_title：Journal of bacteriology

authors： Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

更新日期：2009-02-01 00:00:00

abstract：:We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

journal_title：Journal of bacteriology

authors： Lessner FH,Venters BJ,Keiler KC

更新日期：2007-01-01 00:00:00

abstract：:In gram-negative bacteria, a pathway for aerobic degradation of phenylacetic acid (PAA) that proceeds via phenylacetyl-coenzyme A (CoA) and hydrolytic ring fission plays a central role in the degradation of a range of aromatic compounds. In contrast, the PAA pathway and its role are not well characterized in gram-posi...

journal_title：Journal of bacteriology

authors： Navarro-Llorens JM,Patrauchan MA,Stewart GR,Davies JE,Eltis LD,Mohn WW

更新日期：2005-07-01 00:00:00

abstract：:In Pseudomonas putida DOT-T1E multidrug efflux pumps of the resistance-nodulation-division family make a major contribution to solvent resistance. Two pumps have been identified: TtgABC, expressed constitutively, and TtgDEF, induced by aromatic hydrocarbons. A double mutant lacking both efflux pumps was able to surviv...

journal_title：Journal of bacteriology

authors： Rojas A,Duque E,Mosqueda G,Golden G,Hurtado A,Ramos JL,Segura A

更新日期：2001-07-01 00:00:00

abstract：:The function of AI-2 in many bacteria and the physiological role of LuxS, the enzyme responsible for its production, remain matters of debate. Here, we show that in Staphylococcus aureus the luxS gene forms a monocistronic transcriptional unit under the control of a sigma(70)-dependent promoter. The gene was transcrib...

journal_title：Journal of bacteriology

authors： Doherty N,Holden MT,Qazi SN,Williams P,Winzer K

更新日期：2006-04-01 00:00:00

abstract：:Streptomyces sp. strain HK803 produces six analogues of phoslactomycin (Plm A through Plm F). With the exception of Plm B, these analogues contain a C-18 hydroxyl substituent esterified with a range of short-alkyl-chain carboxylic acids. Deletion of the plmS(2) open reading frame (ORF), showing high sequence similarit...

journal_title：Journal of bacteriology

authors： Ghatge MS,Reynolds KA

更新日期：2005-12-01 00:00:00

abstract：:Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls...

journal_title：Journal of bacteriology

authors： Shockman GD,Martin JT

更新日期：1968-11-01 00:00:00

abstract：:Oxolinic acid reduced RNA synthesis rates whether chromosome supercoiling decreased, increased, or remained unchanged. Thus, inhibition of RNA synthesis by oxolinic acid appears to involve factors other than average DNA supercoiling level. Coumermycin A1 caused RNA synthesis rates to increase or decrease roughly in pa...

journal_title：Journal of bacteriology

authors： Manes SH,Pruss GJ,Drlica K

更新日期：1983-07-01 00:00:00

abstract：:The type II secretion system (main terminal branch of the general secretion pathway) is used by diverse gram-negative bacteria to secrete extracellular proteins. Proteins secreted by this pathway are synthesized with an N-terminal signal peptide which is removed upon translocation across the inner membrane, but the si...

journal_title：Journal of bacteriology

authors： Lindeberg M,Boyd CM,Keen NT,Collmer A

更新日期：1998-03-01 00:00:00

abstract：:Niederpruem, Donald J. (Indiana University Medical Center, Indianapolis). Respiration of basidiospores of Schizophyllum commune. J. Bacteriol. 88:210-215. 1964.-The aerobic metabolism of basidiospores of the wood-rotting mushroom Schizophyllum commune was investigated by use of manometric techniques and specific respi...

journal_title：Journal of bacteriology

authors： NIEDERPRUEM DJ

更新日期：1964-07-01 00:00:00

abstract：:The ftsB gene of Escherichia coli is believed to be involved in cell division. In this report, we show that plasmids containing the nrdB gene could complement the ftsB mutation, suggesting that ftsB is an allele of nrdB. We compared changes in the cell shape of isogenic nrdA, nrdB, ftsB, and pbpB strains at permissive...

journal_title：Journal of bacteriology

authors： Taschner PE,Verest JG,Woldringh CL

更新日期：1987-01-01 00:00:00

abstract：:Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite. ...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ,Coleman JV,Conlon M

更新日期：1975-03-01 00:00:00

abstract：:Thiamine pyrophosphate (TPP) is synthesized de novo in Salmonella typhimurium and is a required cofactor for many enzymes in the cell. Five kinase activities have been implicated in TPP synthesis, which involves joining a 4-methyl-5-(beta-hydroxyethyl)thiazole (THZ) moiety and a 4-amino-5-hydroxymethyl-2-methylpyrimid...

journal_title：Journal of bacteriology

authors： Petersen LA,Downs DM

更新日期：1997-08-01 00:00:00

abstract：:The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...

journal_title：Journal of bacteriology

authors： Mercer AA,Loutit JS

更新日期：1979-10-01 00:00:00

abstract：:Strains monosomic for chromosome I of Saccharomyces cerevisiae contain 25 to 35% fewer rRNA genes than do normal diploid strains. When these strains are repeatedly subcultured, colonies are isolated that have magnified their number of rRNA genes to the diploid amount while remaining monosomic for chromosome I. We have...

journal_title：Journal of bacteriology

authors： Kaback DB,Halvorson HO

更新日期：1978-04-01 00:00:00

abstract：:The mecA-27r gene from Staphylococcus aureus 27r encodes penicillin-binding protein 2a (PBP2a-27r), which causes this strain to be methicillin resistant. Removal or replacement of the N-terminal transmembrane domain had no effect on binding of penicillin, but removal of portions of the putative transglycosylase domain...

journal_title：Journal of bacteriology

authors： Wu CY,Alborn WE Jr,Flokowitsch JE,Hoskins J,Unal S,Blaszczak LC,Preston DA,Skatrud PL

更新日期：1994-01-01 00:00:00