Site-directed mutagenesis of the mecA gene from a methicillin-resistant strain of Staphylococcus aureus.

abstract：:Oceaniovalibus guishaninsula, as a representative of a new genus within the family Rhodobacteraceae, was isolated from surface seawater that was sulfidic. Here, we present the draft genome sequence of the type strain, JLT2003(T). ...

journal_title：Journal of bacteriology

authors： Tang K,Liu K,Jiao N

更新日期：2012-12-01 00:00:00

abstract：:The specificity of chitinase C-1 of Streptomyces griseus HUT 6037 for the hydrolysis of the beta-1,4-glycosidic linkages in partially acetylated chitosan is different from that of other microbial chitinases. In order to study the primary structure of this unique chitinase, the chiC gene specifying chitinase C-1 was cl...

journal_title：Journal of bacteriology

authors： Ohno T,Armand S,Hata T,Nikaidou N,Henrissat B,Mitsutomi M,Watanabe T

更新日期：1996-09-01 00:00:00

abstract：:The lasA gene (whose product is involved in the production of extracellular elastolytic activity) was isolated from a genomic bank containing DNA from Pseudomonas aeruginosa FRD1. Recombinant plasmid pELA1, containing the lasA gene, complemented the temperature-sensitive elastase mutation (lasA1) in P. aeruginosa PAO-...

journal_title：Journal of bacteriology

authors： Goldberg JB,Ohman DE

更新日期：1987-03-01 00:00:00

abstract：:We used a Southern hybridization assay to locate precisely the sites at which DNA replication is arrested in the terminus region of the Escherichia coli chromosome. The assay was based on the properties of restriction fragments that contain stalled replication forks. Replication forks that entered the terminus from th...

journal_title：Journal of bacteriology

authors： Pelletier AJ,Hill TM,Kuempel PL

更新日期：1988-09-01 00:00:00

abstract：:In Escherichia coli, putrescine is metabolized to succinate for use as a carbon and nitrogen source by the putrescine utilization pathway (Puu pathway). One gene in the puu gene cluster encodes a transcription factor, PuuR, which has a helix-turn-helix DNA-binding motif. DNA microarray analysis of an E. coli puuR muta...

journal_title：Journal of bacteriology

authors： Nemoto N,Kurihara S,Kitahara Y,Asada K,Kato K,Suzuki H

更新日期：2012-07-01 00:00:00

abstract：:Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...

journal_title：Journal of bacteriology

authors： Ghosh BK,Lampen JO,Remsen CC

更新日期：1969-11-01 00:00:00

abstract：:The phylum Bacteroidetes is large and diverse, with rapid gliding motility and the ability to digest macromolecules associated with many genera and species. Recently, a novel protein secretion system, the Por secretion system (PorSS), was identified in two members of the phylum, the gliding bacterium Flavobacterium jo...

journal_title：Journal of bacteriology

authors： McBride MJ,Zhu Y

更新日期：2013-01-01 00:00:00

abstract：:The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

journal_title：Journal of bacteriology

authors： Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

更新日期：1982-07-01 00:00:00

abstract：:A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c re...

journal_title：Journal of bacteriology

authors： Tzagoloff A,Akai A,Needleman RB

更新日期：1975-06-01 00:00:00

abstract：:The carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. Increasing concentrations of CO(2) were added as NaHCO(3) to a specially prepared CO(2)-free medium which was tubed and inoculated under nitrogen. Prior depletion of CO(2) in the inoculum was found...

journal_title：Journal of bacteriology

authors： Dehority BA

更新日期：1971-01-01 00:00:00

abstract：:The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...

journal_title：Journal of bacteriology

authors： Inatomi K,Maeda M

更新日期：1988-12-01 00:00:00

abstract：:A fusion constructed between the putative attenuator plus the first 219 nucleotides of the ermC (erythromycin resistance) structural gene and a 5' terminally deleted lacZ gene produced a moderate, basal level of beta-galactosidase which was increased by erythromycin addition. Another construction containing an intact ...

journal_title：Journal of bacteriology

authors： Kirsch DR,Lai MH

更新日期：1984-07-01 00:00:00

abstract：:A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicate...

journal_title：Journal of bacteriology

authors： Sherman LA,van de Putte P

更新日期：1982-04-01 00:00:00

abstract：:Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential...

journal_title：Journal of bacteriology

authors： Jiménez E,Villar-Tajadura MA,Marín M,Fontecha J,Requena T,Arroyo R,Fernández L,Rodríguez JM

更新日期：2012-07-01 00:00:00

abstract：:Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Tra...

journal_title：Journal of bacteriology

authors： Poulsen TS,Chang YY,Hove-Jensen B

更新日期：1999-11-01 00:00:00

abstract：:Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...

journal_title：Journal of bacteriology

authors： Nash HA,Robertson CA,Flamm E,Weisberg RA,Miller HI

更新日期：1987-09-01 00:00:00

abstract：:Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

journal_title：Journal of bacteriology

authors： Iqbal J,Qureshi SA

更新日期：2010-06-01 00:00:00

abstract：:Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent toleranc...

journal_title：Journal of bacteriology

authors： Ramos JL,Duque E,Huertas MJ,Haïdour A

更新日期：1995-07-01 00:00:00

abstract：:A study was made of how the catabolism of a carbon and energy source is affected by the biosynthetic demands of growing bacterial cells. Cultures of Aerobacter aerogenes in l-histidine medium were grown in a chemostat at rates determined by the supply of either sulfate or a required amino acid, l-arginine. It was disc...

journal_title：Journal of bacteriology

authors： Jensen DE,Neidhardt FC

更新日期：1969-04-01 00:00:00

abstract：:Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...

journal_title：Journal of bacteriology

authors： Braunstein M,Griffin TJ IV,Kriakov JI,Friedman ST,Grindley ND,Jacobs WR Jr

更新日期：2000-05-01 00:00:00

abstract：:Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl...

journal_title：Journal of bacteriology

authors： Williams RP,Gott CL,Qadri SM

更新日期：1971-05-01 00:00:00

abstract：:Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...

journal_title：Journal of bacteriology

authors： Kloos DU,DiMarco AA,Elsemore DA,Timmis KN,Ornston LN

更新日期：1995-10-01 00:00:00

abstract：:Hoffman, Heiner (New York University, New York, N.Y.), Jon Valdina, and Michael E. Frank. Effects of high incubation temperature upon the cell wall of Escherichia coli. J. Bacteriol. 91:1635-1637. 1966.-Escherichia coli cells grown at 45 C were found to swell when suspended in distilled water, and were broken down whe...

journal_title：Journal of bacteriology

authors： Hoffman H,Valdina J,Frank ME

更新日期：1966-04-01 00:00:00

abstract：UNLABELLED:Streptococcus pneumoniae is able to integrate exogenous DNA into its genome by natural genetic transformation. Transient accumulation of high levels of the only S. pneumoniae alternative σ factor is insufficient for development of full competence without expression of a second competence-specific protein, Co...

journal_title：Journal of bacteriology

authors： Tovpeko Y,Bai J,Morrison DA

更新日期：2016-08-11 00:00:00

abstract：:Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids. Concatemerization arises from either replication or homologous recombination. Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combinatio...

journal_title：Journal of bacteriology

authors： Vellani TS,Myers RS

更新日期：2003-04-01 00:00:00

abstract：:In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

journal_title：Journal of bacteriology

authors： Oliver EJ,Mortlock RP

更新日期：1971-10-01 00:00:00

abstract：:Group A streptococcus (GAS) produces millions of infections worldwide, including mild mucosal infections, postinfection sequelae, and life-threatening invasive diseases. During infection, GAS readily acquires nutritional iron from host heme and hemoproteins. Here, we identified a new heme importer, named SiaFGH, and i...

journal_title：Journal of bacteriology

authors： Chatterjee N,Cook LCC,Lyles KV,Nguyen HAT,Devlin DJ,Thomas LS,Eichenbaum Z

更新日期：2020-06-25 00:00:00

abstract：:The CO dehydrogenase structural genes (cox) and orf4 are clustered in the transcriptional order coxM--> coxS--> coxL--> orf4 on the 128-kb megaplasmid pHCG3 of the carboxidotroph Oligotropha carboxidovorans OM5. Sequence analysis suggested association of molybdopterin cytosine dinucleotide and flavin adenine dinucleot...

journal_title：Journal of bacteriology

authors： Schübel U,Kraut M,Mörsdorf G,Meyer O

更新日期：1995-04-01 00:00:00

abstract：:In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase. ...

journal_title：Journal of bacteriology

authors： Schué M,Dover LG,Besra GS,Parkhill J,Brown NL

更新日期：2009-01-01 00:00:00

abstract：:Mutants deficient in the production of bacteriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus. Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were ...

journal_title：Journal of bacteriology

authors： Pierson BK,Keith LM,Leovy JG

更新日期：1984-07-01 00:00:00