Bacteriophage SPP1 Chu is an alkaline exonuclease in the SynExo family of viral two-component recombinases.

abstract：:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

journal_title：Journal of bacteriology

authors： Huang AM,Lee JI,King SC,Wilson TH

更新日期：1992-08-01 00:00:00

abstract：:A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase,...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Rosenfeld HJ,Leifer Z,Maas WK

更新日期：1970-03-01 00:00:00

abstract：:The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma class of carbonic anhydrases. A search of databases queried with Mt-Cam revealed that a majority of the homologs comprise a putative subclass (CamH) in which there is major conservation of all of the residues essential for the...

journal_title：Journal of bacteriology

authors： Zimmerman SA,Tomb JF,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three proteins play key roles in the metabolism of elemental sulfur (S(0)): a membrane-bound oxidoreductase complex (MBX), a cytoplasmic coenzyme A-dependent NADPH sulfur oxidoreductase (NSR), and sulfur-induced protein A (SipA). D...

journal_title：Journal of bacteriology

authors： Bridger SL,Clarkson SM,Stirrett K,DeBarry MB,Lipscomb GL,Schut GJ,Westpheling J,Scott RA,Adams MW

更新日期：2011-12-01 00:00:00

abstract：:We have sequenced the rightmost 1,700 base pairs of bacteriophage PRD1. This region encompasses the right early region and completes the sequence of all PRD1 early functions. We have also mapped the 5' initiation site of right early transcripts in vivo and in vitro. This has allowed us to assign gene XII to an open re...

journal_title：Journal of bacteriology

authors： Gerendasy D,Ito J

更新日期：1990-04-01 00:00:00

abstract：:We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

journal_title：Journal of bacteriology

authors： Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

更新日期：1985-01-01 00:00:00

abstract：:Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early st...

journal_title：Journal of bacteriology

authors： Han MJ,Yoon SS,Lee SY

更新日期：2001-01-01 00:00:00

abstract：:PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...

journal_title：Journal of bacteriology

authors： Bergquist PL,Adelberg EA

更新日期：1972-07-01 00:00:00

abstract：:Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridi...

journal_title：Journal of bacteriology

authors： Kimura A,Hirose K,Kariya Y,Nagai S

更新日期：1976-02-01 00:00:00

abstract：:Mutants lacking an enzyme of the oxidative branch of the hexose monophosphate shunt, 6-phosphogluconolactonase (pgl), have been selected as a new class of glucose-negative derivatives of a phosphoglucose isomerase (pgi) mutant. Glucose negativity is not as complete as in mutants lacking phosphoglucose isomerase and gl...

journal_title：Journal of bacteriology

authors： Kupor SR,Fraenkel DG

更新日期：1969-12-01 00:00:00

abstract：:Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

journal_title：Journal of bacteriology

authors： Guo G,Ding Y,Weiss B

更新日期：1997-01-01 00:00:00

abstract：:Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these c...

journal_title：Journal of bacteriology

authors： Miranda-Casoluengo R,Duffy PS,O'Connell EP,Graham BJ,Mangan MW,Prescott JF,Meijer WG

更新日期：2005-05-01 00:00:00

abstract：:The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...

journal_title：Journal of bacteriology

authors： Xu C,Liu B,Hu B,Han Y,Feng L,Allingham JS,Szarek WA,Wang L,Brockhausen I

更新日期：2011-01-01 00:00:00

abstract：:A draft genome sequence of Tsukamurella sp., an aerobic bacterium isolated from a human sputum specimen, is described here. A new virus or provirus, TPA4, was characterized. ...

journal_title：Journal of bacteriology

authors： Oulmi L,Gorlas A,Gimenez G,Robert C,Boulahrouf A,Raoult D,Roux V

更新日期：2012-10-01 00:00:00

abstract：:Phosphorylation of a major 57-kilodalton protein substrate was observed in cell lysates of Spiroplasma melliferum BC3 incubated with [gamma-32P]ATP. Only serine phosphates have been isolated from the acid hydrolysate of the phosphorylated protein. The 57-kilodalton protein substrate was found, to a large extent, in th...

journal_title：Journal of bacteriology

authors： Platt MW,Reizer J,Rottem S

更新日期：1990-05-01 00:00:00

abstract：:Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...

journal_title：Journal of bacteriology

authors： Bennett HP,Clarke DJ

更新日期：2005-01-01 00:00:00

abstract：:Gene conversion, defined as the nonreciprocal transfer of DNA, is one result of homologous recombination. Three steps in recombination could give rise to gene conversion: (i) DNA synthesis for repair of the degraded segment, (ii) Holliday junction migration, leading to heteroduplex formation, and (iii) repair of misma...

journal_title：Journal of bacteriology

authors： Castellanos M,Romero D

更新日期：2009-08-01 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pa...

journal_title：Journal of bacteriology

authors： Mowa MB,Warner DF,Kaplan G,Kana BD,Mizrahi V

更新日期：2009-02-01 00:00:00

abstract：:Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...

journal_title：Journal of bacteriology

authors： Allen NE,MacQuillan AM

更新日期：1969-03-01 00:00:00

abstract：:Entry into the host bacterial cell is one of the least understood steps in the life cycle of bacteriophages. The different envelopes of Gram-negative and Gram-positive bacteria, with a fluid outer membrane and exposing a thick peptidoglycan wall to the environment respectively, impose distinct challenges for bacteriop...

journal_title：Journal of bacteriology

authors： Jakutytė L,Baptista C,São-José C,Daugelavičius R,Carballido-López R,Tavares P

更新日期：2011-09-01 00:00:00

abstract：:Harvey, R. J. (University of California, Davis) and E. B. Collins. Citrate transport system of Streptococcus diacetilactis. J. Bacteriol. 83:1005-1009. 1962.-The uptake of citrate by Streptococcus diacetilactis is mediated by a transport system that was distinguished from passive diffusion by inducibility and kinetics...

journal_title：Journal of bacteriology

authors： HARVEY RJ,COLLINS EB

更新日期：1962-05-01 00:00:00

abstract：:Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolate...

journal_title：Journal of bacteriology

authors： SOMERSON NL,COOK MK

更新日期：1965-08-01 00:00:00

abstract：:Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...

journal_title：Journal of bacteriology

authors： HERMAN A,HALVORSON H

更新日期：1963-04-01 00:00:00

abstract：:Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

journal_title：Journal of bacteriology

authors： Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

更新日期：1998-04-01 00:00:00

abstract：:Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...

journal_title：Journal of bacteriology

authors： Sebulsky MT,Hohnstein D,Hunter MD,Heinrichs DE

更新日期：2000-08-01 00:00:00

abstract：:Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...

journal_title：Journal of bacteriology

authors： Ramamoorthi R,Lidstrom ME

更新日期：1995-01-01 00:00:00

abstract：:The gene GK3045 (741 bp) from Geobacillus kaustophilus HTA426 was cloned, sequenced, and overexpressed into Escherichia coli Rosetta (DE3). The deduced protein was a 30-kDa monomeric esterase with high homology to carboxylesterases from Geobacillus thermoleovorans NY (99% identity) and Geobacillus stearothermophilus (...

journal_title：Journal of bacteriology

authors： Montoro-García S,Martínez-Martínez I,Navarro-Fernández J,Takami H,García-Carmona F,Sánchez-Ferrer A

更新日期：2009-05-01 00:00:00

abstract：:The addition of 1 mM orthophenanthroline (OP) into a mating mixture drastically reduced the production of recombinants. Examination of the effect of OP on each step of conjugation showed that the effect on the following steps could not account for the up to 500-fold reduction of recombinant formation: (i) preliminary ...

journal_title：Journal of bacteriology

authors： Ou JT,Reim R

更新日期：1976-10-01 00:00:00

abstract：:Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...

journal_title：Journal of bacteriology

authors： Tripathi RK,Gottlieb D

更新日期：1969-10-01 00:00:00