The extent of migration of the Holliday junction is a crucial factor for gene conversion in Rhizobium etli.

Abstract:

:Gene conversion, defined as the nonreciprocal transfer of DNA, is one result of homologous recombination. Three steps in recombination could give rise to gene conversion: (i) DNA synthesis for repair of the degraded segment, (ii) Holliday junction migration, leading to heteroduplex formation, and (iii) repair of mismatches in the heteroduplex. There are at least three proteins (RuvAB, RecG, and RadA) that participate in the second step. Their roles have been studied for homologous recombination, but evidence of their relative role in gene conversion is lacking. In this work, we showed the effect on gene conversion of mutations in ruvB, recG, and radA in Rhizobium etli, either alone or in combination, using a cointegration strategy previously developed in our laboratory. The results indicate that the RuvAB system is highly efficient for gene conversion, since its absence provokes smaller gene conversion segments than those in the wild type as well as a shift in the preferred position of conversion tracts. The RecG system possesses a dual role for gene conversion. Inactivation of recG leads to longer gene conversion tracts than those in the wild type, indicating that its activity may hinder heteroduplex extension. However, under circumstances where it is the only migration activity present (as in the ruvB radA double mutant), conversion segments can still be seen, indicating that RecG can also promote gene conversion. RadA is the least efficient system in R. etli but is still needed for the production of detectable gene conversion tracts.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Castellanos M,Romero D

doi

10.1128/JB.00111-09

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

4987-95

issue

15

eissn

0021-9193

issn

1098-5530

pii

JB.00111-09

journal_volume

191

pub_type

杂志文章
  • Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome.

    abstract::A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.4.1087-1092.1994

    authors: Zuerner RL,Stanton TB

    更新日期:1994-02-01 00:00:00

  • Effect of bacteriophage lambda infection on synthesis of groE protein and other Escherichia coli proteins.

    abstract::We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1050-1063.1982

    authors: Drahos DJ,Hendrix RW

    更新日期:1982-03-01 00:00:00

  • A cytochrome cbb3 (cytochrome c) terminal oxidase in Azospirillum brasilense Sp7 supports microaerobic growth.

    abstract::Spectral analysis indicated the presence of a cytochrome cbb3 oxidase under microaerobic conditions in Azospirillum brasilense Sp7 cells. The corresponding genes (cytNOQP) were isolated by using PCR. These genes are organized in an operon, preceded by a putative anaerobox. The phenotype of an A. brasilense cytN mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.21.5689-5696.1998

    authors: Marchal K,Sun J,Keijers V,Haaker H,Vanderleyden J

    更新日期:1998-11-01 00:00:00

  • Functional complementation between bacterial MDR-like export systems: colicin V, alpha-hemolysin, and Erwinia protease.

    abstract::The antibacterial protein Colicin V (ColV) is secreted from gram-negative bacteria by a signal sequence-independent pathway. The proteins that mediate the export of ColV share sequence similarities with components from other signal sequence-independent export systems such as those for alpha-hemolysin (Hly) and Erwinia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7549-7556.1991

    authors: Fath MJ,Skvirsky RC,Kolter R

    更新日期:1991-12-01 00:00:00

  • RflM functions as a transcriptional repressor in the autogenous control of the Salmonella Flagellar master operon flhDC.

    abstract::Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00728-13

    authors: Singer HM,Erhardt M,Hughes KT

    更新日期:2013-09-01 00:00:00

  • Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.

    abstract::We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4488-4500.1995

    authors: Strohmaier H,Remler P,Renner W,Högenauer G

    更新日期:1995-08-01 00:00:00

  • Bacteriophage infection in rod-shaped gram-positive bacteria: evidence for a preferential polar route for phage SPP1 entry in Bacillus subtilis.

    abstract::Entry into the host bacterial cell is one of the least understood steps in the life cycle of bacteriophages. The different envelopes of Gram-negative and Gram-positive bacteria, with a fluid outer membrane and exposing a thick peptidoglycan wall to the environment respectively, impose distinct challenges for bacteriop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05104-11

    authors: Jakutytė L,Baptista C,São-José C,Daugelavičius R,Carballido-López R,Tavares P

    更新日期:2011-09-01 00:00:00

  • DnaA protein is required for replication of the minimal replicon of the broad-host-range plasmid RK2 in Escherichia coli.

    abstract::The minimal origin of replication of the broad-host-range plasmid RK2 has two potential recognition sequences for the DnaA protein of Escherichia coli. DNA transfer by transformation into a dnaA-null mutant of E. coli showed that DnaA protein is needed for replication or maintenance of mini-RK2. We isolated and purifi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4703-4709.1987

    authors: Gaylo PJ,Turjman N,Bastia D

    更新日期:1987-10-01 00:00:00

  • Oxidation of phenolate siderophores by the multicopper oxidase encoded by the Escherichia coli yacK gene.

    abstract::A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.16.4866-4875.2001

    authors: Kim C,Lorenz WW,Hoopes JT,Dean JF

    更新日期:2001-08-01 00:00:00

  • Thickness and elasticity of gram-negative murein sacculi measured by atomic force microscopy.

    abstract::Atomic force microscopy was used to measure the thickness of air-dried, collapsed murein sacculi from Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Air-dried sacculi from E. coli had a thickness of 3.0 nm, whereas those from P. aeruginosa were 1.5 nm thick. When rehydrated, the sacculi of both bacteria swelle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.22.6865-6875.1999

    authors: Yao X,Jericho M,Pink D,Beveridge T

    更新日期:1999-11-01 00:00:00

  • Gene fusion is a possible mechanism underlying the evolution of STA1.

    abstract::DNA from the STA1 (extracellular glucoamylase) gene of Saccharomyces diastaticus was used as a probe to enable the cloning by colony hybridization of three DNA fragments from Saccharomyces cerevisiae; these were designated S1, S2, and SGA (intracellular, sporulation-specific glucoamylase gene). To examine the evolutio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.2142-2149.1987

    authors: Yamashita I,Nakamura M,Fukui S

    更新日期:1987-05-01 00:00:00

  • Mutations in Rhizobium phaseoli that lead to arrested development of infection threads.

    abstract::Two Rhizobium phaseoli mutants, isolated previously by Tn5 mutagenesis, elicited infection threads which ceased development prematurely, usually within root hairs. These infection threads were wide, globular, and otherwise altered in morphology, compared with normal infection threads. Anatomy and division of the root ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1392-1401.1986

    authors: Noel KD,Vandenbosch KA,Kulpaca B

    更新日期:1986-12-01 00:00:00

  • A second global regulator gene (arcB) mediating repression of enzymes in aerobic pathways of Escherichia coli.

    abstract::In Escherichia coli anaerobic growth lowers the basal or induced levels of numerous enzymes associated with aerobic metabolism. Mutations in arcA (dye) at min 0 relieve this pleiotropic anaerobic repression and render the cell sensitive to the redox dye toluidine blue. In this study we identified a second pleiotropic ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.868-873.1989

    authors: Iuchi S,Cameron DC,Lin EC

    更新日期:1989-02-01 00:00:00

  • Structural Characterization of the Fla2 Flagellum of Rhodobacter sphaeroides.

    abstract:UNLABELLED:Rhodobacter sphaeroides is a free-living alphaproteobacterium that contains two clusters of functional flagellar genes in its genome: one acquired by horizontal gene transfer (fla1) and one that is endogenous (fla2). We have shown that the Fla2 system is normally quiescent and under certain conditions produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00170-15

    authors: de la Mora J,Uchida K,del Campo AM,Camarena L,Aizawa S,Dreyfus G

    更新日期:2015-09-01 00:00:00

  • Novel one-step cloning vector with a transposable element: application to the Myxococcus xanthus genome.

    abstract::A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.270-275.1985

    authors: Furuichi T,Inouye M,Inouye S

    更新日期:1985-10-01 00:00:00

  • VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to exsB of Pseudomonas aeruginosa.

    abstract::Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4230-4237.1995

    authors: Allaoui A,Scheen R,Lambert de Rouvroit C,Cornelis GR

    更新日期:1995-08-01 00:00:00

  • Complete genome sequence of Lactobacillus plantarum JDM1.

    abstract::Lactobacillus plantarum is a lactic acid bacterium (LAB) species commonly used as a probiotic. We have sequenced the genome of Lactobacillus plantarum JDM1, which is a Chinese commercial LAB with several probiotic functions, using a GS 20 system. We recommend that each commercial probiotic strain should undergo comple...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00587-09

    authors: Zhang ZY,Liu C,Zhu YZ,Zhong Y,Zhu YQ,Zheng HJ,Zhao GP,Wang SY,Guo XK

    更新日期:2009-08-01 00:00:00

  • Influences of growth substrates and oxygen on the electron transport system in Acinetobacter sp. HO1-N.

    abstract::The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various concentrations of oxygen or types of growth substrates. Spectrophotometric analysis revealed that the quantity and type...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.3.859-868.1980

    authors: Ensley BD Jr,Finnerty WR

    更新日期:1980-06-01 00:00:00

  • DNA binding: a novel function of Pseudomonas aeruginosa type IV pili.

    abstract::The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1455-1464.2005

    authors: van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

    更新日期:2005-02-01 00:00:00

  • Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination.

    abstract::Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.1.108-121.1976

    authors: Canosi U,Siccardi AG,Falaschi A,Mazza G

    更新日期:1976-04-01 00:00:00

  • Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus.

    abstract::Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00574-06

    authors: Kasai K,Nishizawa T,Takahashi K,Hosaka T,Aoki H,Ochi K

    更新日期:2006-10-01 00:00:00

  • Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR.

    abstract:UNLABELLED:Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00820-15

    authors: Uhde A,Brühl N,Goldbeck O,Matano C,Gurow O,Rückert C,Marin K,Wendisch VF,Krämer R,Seibold GM

    更新日期:2016-07-28 00:00:00

  • Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli.

    abstract::Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.17.5549-5559.1992

    authors: Caparrós M,Pisabarro AG,de Pedro MA

    更新日期:1992-09-01 00:00:00

  • Comparison of the two isofunctional enol-lactone hydrolases from Acinetobacter calcoaceticus.

    abstract::The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.369-374.1971

    authors: Katagiri M,Wheelis ML

    更新日期:1971-05-01 00:00:00

  • Rhizobium leguminosarum mutants incapable of normal extracellular polysaccharide production.

    abstract::Mutants of Rhizobium leguminosarum which are deficient in normal polysaccharide production have been isolated and characterized. A correlation between diminished production of extracellular polysaccharide and reduced infection and nodulation efficiency has been observed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1454-1456.1980

    authors: Napoli C,Albersheim P

    更新日期:1980-03-01 00:00:00

  • Anaerobic regulation of Bacillus subtilis Krebs cycle genes.

    abstract::Krebs cycle enzyme activity in Bacillus subtilis was examined under aerobic and anaerobic conditions. Citrate synthase and aconitase activities in cells grown anaerobically in the presence of nitrate were reduced by as much as 10- and 30-fold, respectively, from levels observed under aerobic culture conditions. The ma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.13.3304-3311.1998

    authors: Nakano MM,Zuber P,Sonenshein AL

    更新日期:1998-07-01 00:00:00

  • Adaptation to sulfonamide resistance in Neisseria meningitidis may have required compensatory changes to retain enzyme function: kinetic analysis of dihydropteroate synthases from N. meningitidis expressed in a knockout mutant of Escherichia coli.

    abstract::Previously, the effects of three point mutations (at amino acid positions 31, 84, and 194) in the gene coding for a sulfonamide-resistant dihydropteroate synthase of Neisseria meningitidis were analyzed by site-directed mutagenesis. Changes at positions 31 and 194 abolished the phenotypic expression of sulfonamide res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.3.831-837.1997

    authors: Fermér C,Swedberg G

    更新日期:1997-02-01 00:00:00

  • Cellular location of origin and terminus of replication in Bacillus subtilis.

    abstract::The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.3.895-899.1985

    authors: Sonnenfeld EM,Koch AL,Doyle RJ

    更新日期:1985-09-01 00:00:00

  • Regulation of synthesis of the branched-chain amino acids and cognate aminoacyl-transfer ribonucleic acid synthetases of Escherichia coli: a common regulatory element.

    abstract::Regulation of isoleucine, valine, and leucine biosynthesis and isoleucyl-, valyl-, and leucyl-transfer ribonucleic acid (tRNA) synthetase formation was examined in two mutant strains of Escherichia coli. One mutant was selected for growth resistance to the isoleucine analogue, ketomycin, and the other was selected for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.3.1380-1386.1974

    authors: Jackson J,Williams LS,Umbarger HE

    更新日期:1974-12-01 00:00:00

  • Structural characterization and corepressor binding of the Escherichia coli purine repressor.

    abstract::The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.19.6207-6214.1992

    authors: Choi KY,Zalkin H

    更新日期:1992-10-01 00:00:00