Abstract:
:Gene conversion, defined as the nonreciprocal transfer of DNA, is one result of homologous recombination. Three steps in recombination could give rise to gene conversion: (i) DNA synthesis for repair of the degraded segment, (ii) Holliday junction migration, leading to heteroduplex formation, and (iii) repair of mismatches in the heteroduplex. There are at least three proteins (RuvAB, RecG, and RadA) that participate in the second step. Their roles have been studied for homologous recombination, but evidence of their relative role in gene conversion is lacking. In this work, we showed the effect on gene conversion of mutations in ruvB, recG, and radA in Rhizobium etli, either alone or in combination, using a cointegration strategy previously developed in our laboratory. The results indicate that the RuvAB system is highly efficient for gene conversion, since its absence provokes smaller gene conversion segments than those in the wild type as well as a shift in the preferred position of conversion tracts. The RecG system possesses a dual role for gene conversion. Inactivation of recG leads to longer gene conversion tracts than those in the wild type, indicating that its activity may hinder heteroduplex extension. However, under circumstances where it is the only migration activity present (as in the ruvB radA double mutant), conversion segments can still be seen, indicating that RecG can also promote gene conversion. RadA is the least efficient system in R. etli but is still needed for the production of detectable gene conversion tracts.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Castellanos M,Romero Ddoi
10.1128/JB.00111-09subject
Has Abstractpub_date
2009-08-01 00:00:00pages
4987-95issue
15eissn
0021-9193issn
1098-5530pii
JB.00111-09journal_volume
191pub_type
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pub_type: 杂志文章
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更新日期:1995-08-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1992-09-01 00:00:00
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更新日期:1980-03-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1974-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1992-10-01 00:00:00