Cellular location of origin and terminus of replication in Bacillus subtilis.

abstract：:Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...

journal_title：Journal of bacteriology

authors： Wu H,Kato J,Kuroda A,Ikeda T,Takiguchi N,Ohtake H

更新日期：2000-06-01 00:00:00

abstract：:In Escherichia coli, the GlpT transporter, a member of the major facilitator superfamily, moves external glycerol 3-phosphate (G3P) into the cytoplasm in exchange for cytoplasmic phosphate. Study of intact cells showed that both GlpT and HisGlpT, a variant with an N-terminal six-histidine tag, are inhibited (50% inhib...

journal_title：Journal of bacteriology

authors： Fann MC,Busch A,Maloney PC

更新日期：2003-07-01 00:00:00

abstract：:The transfer of plasmid DNA molecules between bacterial cells is achieved by a large array of conjugative transfer proteins which assemble into both cytoplasmic and membrane-associated complexes. TrhC is a membrane-associated protein that is required for the transfer of the IncHI1 resistance plasmid R27. Homologous pr...

journal_title：Journal of bacteriology

authors： Gilmour MW,Taylor DE

更新日期：2004-03-01 00:00:00

abstract：:Mutants of Rhizobium leguminosarum which are deficient in normal polysaccharide production have been isolated and characterized. A correlation between diminished production of extracellular polysaccharide and reduced infection and nodulation efficiency has been observed. ...

journal_title：Journal of bacteriology

authors： Napoli C,Albersheim P

更新日期：1980-03-01 00:00:00

abstract：:The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

journal_title：Journal of bacteriology

authors： Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

更新日期：2014-02-01 00:00:00

abstract：:Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could mi...

journal_title：Journal of bacteriology

authors： O'Shea TM,Klein AH,Geszvain K,Wolfe AJ,Visick KL

更新日期：2006-12-01 00:00:00

abstract：:The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels. We therefore directly measured the NAD+ and NADH levels for cultures growing aerobically ...

journal_title：Journal of bacteriology

authors： Leonardo MR,Dailly Y,Clark DP

更新日期：1996-10-01 00:00:00

abstract：:The bacterial peptidoglycan consists of glycan chains of repeating beta-1,4-linked N-acetylglucosaminyl-N-acetylmuramyl units cross-linked through short peptide chains. The polymerization of the glycans, or glycosyltransfer (GT), and transpeptidation (TP) are catalyzed by bifunctional penicillin-binding proteins (PBPs...

journal_title：Journal of bacteriology

authors： Di Guilmi AM,Dessen A,Dideberg O,Vernet T

更新日期：2003-08-01 00:00:00

abstract：:Rickettsia heilongjiangensis is an emerging tick-transmitted human pathogen causing far-Eastern spotted fever. Here we report the complete sequence and the main features of the genome of R. heilongjiangensis (strain 054). ...

journal_title：Journal of bacteriology

authors： Duan C,Tong Y,Huang Y,Wang X,Xiong X,Wen B

更新日期：2011-10-01 00:00:00

abstract：:The carbon monoxide (CO) dehydrogenase activity band from Clostridium pasteurianum was shown to contain nickel by in situ activity staining of polyacrylamide gels. However, the majority of the nickel in cell extracts was found to electrophorese independently of CO dehydrogenase. Comparative analysis with Clostridium t...

journal_title：Journal of bacteriology

authors： Drake HL

更新日期：1982-02-01 00:00:00

abstract：:The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

journal_title：Journal of bacteriology

authors： Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

更新日期：2008-02-01 00:00:00

abstract：:The first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a c...

journal_title：Journal of bacteriology

authors： Vary PS,Garbe JC,Franzen M,Frampton EW

更新日期：1982-03-01 00:00:00

abstract：:The activities of DNA methyltransferases are important for a variety of cellular functions in bacteria. In this study, we developed a modified high-throughput technique called methyl homopolymer tail mediated sequencing (methyl HTM-seq) to identify the undermethylated sites in the Vibrio cholerae genome for the two DN...

journal_title：Journal of bacteriology

authors： Dalia AB,Lazinski DW,Camilli A

更新日期：2013-05-01 00:00:00

abstract：:As a result of serial exposures to a mutagenic agent, N-methyl-N'-nitro-N-nitrosoguanidine, the yield of enterotoxin A produced by the last mutant in the series was increased nearly 20-fold over the amount produced by the parent Staphylococcus aureus 100. ...

journal_title：Journal of bacteriology

authors： Friedman ME,Howard MB

更新日期：1971-04-01 00:00:00

abstract：:Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...

journal_title：Journal of bacteriology

authors： Kitagawa M,Wada C,Yoshioka S,Yura T

更新日期：1991-07-01 00:00:00

abstract：:Mu promoters active during the lytic cycle were located by isolating RNA at various times after induction of Mu prophages, radiolabeling it by capping in vitro, and hybridizing it to Mu DNA fragments on Southern blots. Signals were detected from four new promoters in addition to the previously characterized Pe (early)...

journal_title：Journal of bacteriology

authors： Stoddard SF,Howe MM

更新日期：1989-06-01 00:00:00

abstract：:The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...

journal_title：Journal of bacteriology

authors： Wang RJ,Morse HG,Morse ML

更新日期：1970-12-01 00:00:00

abstract：:During the progression of Mycoplasma hominis cultures from the early logarithmic phase to the stationary phase of growth, the total phospholipid content of the cell membranes decreased. Measurement of the amount of the various phospholipids during the growth cycle showed that a decrease in the phosphatidylglycerol (PG...

journal_title：Journal of bacteriology

authors： Rottem S,Greenberg AS

更新日期：1975-02-01 00:00:00

abstract：:Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymid...

journal_title：Journal of bacteriology

authors： Buxton RS

更新日期：1975-02-01 00:00:00

abstract：:The tetracycline resistance element of plasmid pBR322 partially complements the potassium transport defect of Escherichia coli K-12 mutants having markedly impaired K+ transport. The plasmid increases K+ transport. The Tn10 element does not result in increased transport, demonstrating that the effect is not general fo...

journal_title：Journal of bacteriology

authors： Dosch DC,Salvacion FF,Epstein W

更新日期：1984-12-01 00:00:00

abstract：:Two hundred strains of Escherichia coli harboring Filv+ plasmids which carry a segment of the Salmonella typhimurium chromosome were isolated independently. Among them, two strains were found to harbor F' plasmids that are able to replicate in Hfr cells of E. coli; i.e., they carry a site designated poh (permissive on...

journal_title：Journal of bacteriology

authors： Joh K,Hiraga S

更新日期：1979-05-01 00:00:00

abstract：:Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...

journal_title：Journal of bacteriology

authors： Seele J,Singpiel A,Spoerry C,von Pawel-Rammingen U,Valentin-Weigand P,Baums CG

更新日期：2013-03-01 00:00:00

abstract：:Strain DCB-1 is a strict anaerobe capable of reductive dehalogenation. We elucidated metabolic processes in DCB-1 which may be related to dehalogenation and which further characterize the organism physiologically. Sulfoxy anions and CO2 were used by DCB-1 as catabolic electron acceptors. With suitable electron donors,...

journal_title：Journal of bacteriology

authors： Mohn WW,Tiedje JM

更新日期：1990-04-01 00:00:00

abstract：:Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasm...

journal_title：Journal of bacteriology

authors： FOX EN

更新日期：1963-03-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum. Motility has been implicated as a virulence determinant in some animal models of cholera, but the relationship between motility and virulence has not yet been clearly defined. We have begun...

journal_title：Journal of bacteriology

authors： Klose KE,Mekalanos JJ

更新日期：1998-01-01 00:00:00

abstract：:Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that ...

journal_title：Journal of bacteriology

authors： Wong B,Leeson S,Grindle S,Magee B,Brooks E,Magee PT

更新日期：1995-06-01 00:00:00

abstract：:Type 3 fimbriae are adhesive organelles found in enterobacterial pathogens. The fimbriae promote biofilm formation on biotic and abiotic surfaces; however, the exact identity of the receptor for the type 3 fimbriae adhesin, MrkD, remains elusive. We analyzed naturally occurring structural and functional variabilities ...

journal_title：Journal of bacteriology

authors： Stahlhut SG,Chattopadhyay S,Kisiela DI,Hvidtfeldt K,Clegg S,Struve C,Sokurenko EV,Krogfelt KA

更新日期：2013-12-01 00:00:00

abstract：:A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...

journal_title：Journal of bacteriology

authors： MacNeil D

更新日期：1981-04-01 00:00:00

abstract：:Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...

journal_title：Journal of bacteriology

authors： Lonhienne T,Mavromatis K,Vorgias CE,Buchon L,Gerday C,Bouriotis V

更新日期：2001-03-01 00:00:00

abstract：:Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

journal_title：Journal of bacteriology

authors： Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

更新日期：2014-04-01 00:00:00