Abstract:
:The transfer of plasmid DNA molecules between bacterial cells is achieved by a large array of conjugative transfer proteins which assemble into both cytoplasmic and membrane-associated complexes. TrhC is a membrane-associated protein that is required for the transfer of the IncHI1 resistance plasmid R27. Homologous proteins are encoded in all known conjugative systems, and each contains characteristic nucleoside triphosphate (NTP)-binding domains. An assembly of R27-encoded proteins was previously visualized by use of a TrhC-green fluorescent protein fusion, which appeared as discrete membrane-associated fluorescent foci. We have utilized this experimental system to determine the requirements for assembly of this TrhC-associated protein complex, and we found that 12 of the other 18 R27 transfer proteins are required for focus formation. An individual focus possibly represents a subassembly comprised of some or all of these transfer proteins. These data support the notion that the transfer apparatus is a multicomponent structure. In contrast, substitutions and deletions within TrhC NTP-binding motifs had minor effects on focus formation, but these mutations did affect plasmid transfer and bacteriophage susceptibility. These results indicate that TrhC requires intact NTP-binding motifs to function during conjugative transfer but that these motifs are not essential for the assembly of TrhC into a complex with other transfer proteins.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gilmour MW,Taylor DEdoi
10.1128/jb.186.6.1606-1613.2004keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
1606-13issue
6eissn
0021-9193issn
1098-5530journal_volume
186pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/JB.155.2.634-642.1983
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1621-1623.1975
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doi:10.1128/jb.173.24.7942-7949.1991
更新日期:1991-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/jb.175.11.3361-3371.1993
更新日期:1993-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.1208-1210.1972
更新日期:1972-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1968-08-01 00:00:00
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更新日期:1979-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1982-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.16.4794-4800.1996
更新日期:1996-08-01 00:00:00