Cloning, sequences, and characterization of two chitinase genes from the Antarctic Arthrobacter sp. strain TAD20: isolation and partial characterization of the enzymes.

abstract：:The flavodoxins constitute a highly conserved family of small, acidic electron transfer proteins with flavin mononucleotide prosthetic groups. They are found in prokaryotes and in red and green algae, where they provide electrons at low potentials for the reduction of nitrogen by nitrogenase, for the light-dependent r...

journal_title：Journal of bacteriology

authors： Osborne C,Chen LM,Matthews RG

更新日期：1991-03-01 00:00:00

abstract：:Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

journal_title：Journal of bacteriology

authors： HOWARD DH

更新日期：1964-01-01 00:00:00

abstract：:Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...

journal_title：Journal of bacteriology

authors： Daumy GO,McColl AS,Andrews GC

更新日期：1980-01-01 00:00:00

abstract：:Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature s...

journal_title：Journal of bacteriology

authors： Eller C,Ordal ZJ

更新日期：1972-09-01 00:00:00

abstract：:Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

journal_title：Journal of bacteriology

authors： Franzetti B,Sohlberg B,Zaccai G,von Gabain A

更新日期：1997-02-01 00:00:00

abstract：:The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...

journal_title：Journal of bacteriology

authors： Leelakriangsak M,Kobayashi K,Zuber P

更新日期：2007-03-01 00:00:00

abstract：:During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...

journal_title：Journal of bacteriology

authors： Severinova E,Severinov K

更新日期：2006-05-01 00:00:00

abstract：:A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...

journal_title：Journal of bacteriology

authors： Rebers PA,Heddleston KL,Rhoades KR

更新日期：1967-01-01 00:00:00

abstract：:Escherichia coli suffered 95 to 100% lethality when exposed to 1.0 mM paraquat for 30 min at 37 degrees C in aerobic nutrient broth medium but did not lose viability when the exposure was done in Vogel Bonner or tryptic soy yeast extract medium. Paraquat was, however, bacteriostatic in all of these media. Salts, added...

journal_title：Journal of bacteriology

authors： Kitzler J,Fridovich I

更新日期：1986-07-01 00:00:00

abstract：:Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls...

journal_title：Journal of bacteriology

authors： Shockman GD,Martin JT

更新日期：1968-11-01 00:00:00

abstract：:To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

journal_title：Journal of bacteriology

authors： Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

更新日期：1997-11-01 00:00:00

abstract：:In Bacillus subtilis, carbon catabolite repression (CCR) of many genes is mediated at cis-acting carbon repression elements (cre) by the catabolite repressor protein CcpA. Mutations in transcription-repair coupling factor (mfd) partially relieve CCR at cre sites located downstream of transcriptional start sites by abo...

journal_title：Journal of bacteriology

authors： Zalieckas JM,Wray LV Jr,Fisher SH

更新日期：1998-12-01 00:00:00

abstract：:We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

journal_title：Journal of bacteriology

authors： Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

更新日期：2007-01-01 00:00:00

abstract：:After the dormancy of Saccharomyces cerevisiae ascospores had been broken, the synthesis of proteins was observed first, followed rapidly by synthesis of ribonucleic acid (RNA) and much later by deoxyribonucleic acid (DNA) synthesis. Phosphoglucomutase activity increased in a periodic (step) fashion, whereas the activ...

journal_title：Journal of bacteriology

authors： Rousseau P,Halvorson HO

更新日期：1973-03-01 00:00:00

abstract：:Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of...

journal_title：Journal of bacteriology

authors： Hilger F,Mortimer RK

更新日期：1980-01-01 00:00:00

abstract：:Strains carrying either the polA1 or resA1 mutation are deficient in DNA polymerase I, and the polA1 and resA1 mutations do not complement in merozygotes. The effect of these mutations in otherwise identical genetic backgrounds was studied: after ultraviolet irradiation both strains degrade their DNA more rapidly and ...

journal_title：Journal of bacteriology

authors： Glickman BW,van Sluis CA,van der Maas G,Rörsch A

更新日期：1973-06-01 00:00:00

abstract：:A lipoteichoic acid (LTA) was extracted from Listeria monocytogenes (serotype 1) by phenol-water partition and isolated by gel-filtration chromatography. The LTA exhibited amphiphilic properties by changes in gel-filtration mobility in the presence of detergent buffers and after mild base hydrolysis. In a hemagglutina...

journal_title：Journal of bacteriology

authors： Hether NW,Jackson LL

更新日期：1983-11-01 00:00:00

abstract：:A genetic determinant conferring on Escherichia coli the ability to utilize citrate as a sole source of carbon and energy was subcloned into pBR322 from a naturally occurring, citrate utilization (Cit+) plasmid, pOH30221, and was localized to a 1.6-kilobase region by cloning and subsequent deletion analysis. Genetic e...

journal_title：Journal of bacteriology

authors： Hirato T,Shinagawa M,Ishiguro N,Sato G

更新日期：1984-10-01 00:00:00

abstract：UNLABELLED:The Pseudomonas aeruginosa cyclic AMP (cAMP)-Vfr system (CVS) is a global regulator of virulence gene expression. Regulatory targets include type IV pili, secreted proteases, and the type III secretion system (T3SS). The mechanism by which CVS regulates T3SS gene expression remains undefined. Single-cell exp...

journal_title：Journal of bacteriology

authors： Marsden AE,Intile PJ,Schulmeyer KH,Simmons-Patterson ER,Urbanowski ML,Wolfgang MC,Yahr TL

更新日期：2016-04-14 00:00:00

abstract：:Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies ident...

journal_title：Journal of bacteriology

authors： Roy S,Ajitkumar P

更新日期：2005-04-01 00:00:00

abstract：:Insertion of Tn7 in nine independently derived isolates occurred in a single small region of the Caulobacter crescentus chromosome, suggesting that Tn7 insertion occurs at or near a single site. The order of markers in this region of the Caulobacter chromosome was shown to be flaA-argG-Tn7. ...

journal_title：Journal of bacteriology

authors： Ely B

更新日期：1982-08-01 00:00:00

abstract：:Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis...

journal_title：Journal of bacteriology

authors： Hutchison HT,Hartwell LH

更新日期：1967-11-01 00:00:00

abstract：:Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...

journal_title：Journal of bacteriology

authors： Kasai K,Nishizawa T,Takahashi K,Hosaka T,Aoki H,Ochi K

更新日期：2006-10-01 00:00:00

abstract：:In earlier studies from this laboratory, Xanthomonas campestris pv. glycines was found to exhibit a nutrition stress-related postexponential rapid cell death (RCD). The RCD was exhibited in protein-rich media but not in starch or other minimal media. This RCD in X. campestris pv. glycines was found to display features...

journal_title：Journal of bacteriology

authors： Raju KK,Gautam S,Sharma A

更新日期：2006-08-01 00:00:00

abstract：:Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...

journal_title：Journal of bacteriology

authors： Hoffmann HP,Szabo A,Avers CJ

更新日期：1970-10-01 00:00:00

abstract：:The protein surface layers on the cell walls of mosquito-pathogenic and nonpathogenic Bacillus sphaericus strains were studied by structural, biochemical, and serological methods. The surface structure of two representative insect-pathogenic strains had the form of a delicate linear array with a repeat interval of 5 n...

journal_title：Journal of bacteriology

authors： Lewis LO,Yousten AA,Murray RG

更新日期：1987-01-01 00:00:00

abstract：:A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

journal_title：Journal of bacteriology

authors： Kelly R,Kwon-Chung KJ

更新日期：1992-01-01 00:00:00

abstract：:The yeast metallothionein gene CUP1 was cloned into a bacterial expression system to achieve efficient, controlled expression of the stable, unprocessed protein product. The Escherichia coli-synthesized yeast metallothionein bound copper, cadmium, and zinc, indicating that the protein was functional. Furthermore, E. c...

journal_title：Journal of bacteriology

authors： Berka T,Shatzman A,Zimmerman J,Strickler J,Rosenberg M

更新日期：1988-01-01 00:00:00

abstract：:Human dental biofilm communities comprise several species, which can interact cooperatively or competitively. Bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. Lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of ...

journal_title：Journal of bacteriology

authors： Periasamy S,Kolenbrander PE

更新日期：2010-06-01 00:00:00

abstract：:We previously described 14 rare spontaneous morphological mutants of Candida albicans that were associated with chromosomal aberrations (E. P. Rustchenko-Bulgac, F. Sherman, and J. B. Hicks, J. Bacteriol. 172:1276-1283, 1990). Improved conditions for separation of chromosomes, as well as hybridization probes, were use...

journal_title：Journal of bacteriology

authors： Rustchenko-Bulgac EP

更新日期：1991-10-01 00:00:00