Abstract:
:Strain DCB-1 is a strict anaerobe capable of reductive dehalogenation. We elucidated metabolic processes in DCB-1 which may be related to dehalogenation and which further characterize the organism physiologically. Sulfoxy anions and CO2 were used by DCB-1 as catabolic electron acceptors. With suitable electron donors, sulfate and thiosulfate were reduced to sulfide. Sulfate and thiosulfate supported growth with formate or hydrogen as the electron donor and thus are probably respiratory electron acceptors. Other electron donors supporting growth with sulfate were CO, lactate, pyruvate, butyrate, and 3-methoxybenzoate. Thiosulfate also supported growth without an additional electron donor, being disproportionated to sulfide and sulfate. In the absence of other electron acceptors, CO2 reduction to acetate plus cell material was coupled to pyruvate oxidation to acetate plus CO2. Pyruvate could not be fermented without an electron acceptor. Carbon monoxide dehydrogenase activity was found in whole cells, indicating that CO2 reduction probably occurred via the acetyl coenzyme A pathway. Autotrophic growth occurred on H2 plus thiosulfate or sulfate. Diazotrophic growth occurred, and whole cells had nitrogenase activity. On the basis of these physiological characteristics, DCB-1 is a thiosulfate-disproportionating bacterium unlike those previously described.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Mohn WW,Tiedje JMdoi
10.1128/jb.172.4.2065-2070.1990subject
Has Abstractpub_date
1990-04-01 00:00:00pages
2065-70issue
4eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::Studies on the composition of total fatty acids of Alcaligenes faecalis harvested at different growth phases have been carried out. Ability of the organism to desaturate palmitic and stearic acid has also been tested. The organism contained palmitic (16:0), stearic (18:0), palmitoleic (16:1), cis-vaccenic (18:1), cycl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.618-624.1973
更新日期:1973-05-01 00:00:00
abstract::Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three alpha subunits) and approximately 60 beta subunits (A. Bacher, R. Bauer, U. Eggers, H. Harders, and H. Schnepple, p. 729-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.476-482.1978
更新日期:1978-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.183.7.2372-2375.2001
更新日期:2001-04-01 00:00:00
abstract::Open reading frame orf192, which is located immediately upstream of the aerobic repressor gene crtJ, was genetically and biochemically demonstrated to code for a second aerobic repressor (AerR) of photosynthesis gene expression in Rhodobacter capsulatus. Promoter-mapping studies indicate that crtJ has its own promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.10.2805-2814.2002
更新日期:2002-05-01 00:00:00
abstract::Metal-dependent superoxide dismutases (SODs) with a specific requirement for a manganese or iron ion for catalytic activity and copper- and zinc-dependent enzymes are essential for detoxification of superoxide anion radicals. Genome sequence analyses predict the existence of a nickel-dependent enzyme (NiSOD) as the un...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7821-7825.2004
更新日期:2004-11-01 00:00:00
abstract::Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2031-2034.1968
更新日期:1968-06-01 00:00:00
abstract::Cyanobacteria are monophyletic organisms that perform oxygenic photosynthesis. While they exhibit great diversity, they have a common set of genes. However, the essentiality of them for viability has hampered the elucidation of their functions. One example of these genes is cyabrB1 (also known as calA in Anabaena sp. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00244-19
更新日期:2019-08-08 00:00:00
abstract::Extracellular fibrils are involved in cell cohesion and cell development in Myxococcus xanthus. One group of social motility mutants, Dsp, is unable to produce extracellular fibrils; these mutants also lose the abilities to cohere and to develop. Extracellular fibrils isolated from vegetative wild-type cells and added...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7190-7196.1994
更新日期:1994-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3864-3871.1991
更新日期:1991-06-01 00:00:00
abstract::ParB is one of two P1-encoded proteins that are required for active partition of the P1 prophage in Escherichia coli. To probe the native domain structure of ParB, we performed limited proteolytic digestions of full-length ParB, as well as of several N-terminal and C-terminal deletion fragments of ParB. The C-terminal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.5898-5908.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4508-4518.2003
更新日期:2003-08-01 00:00:00
abstract::Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.788-795.1981
更新日期:1981-02-01 00:00:00
abstract::The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.618-627.1970
更新日期:1970-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5127-5131.1992
更新日期:1992-08-01 00:00:00
abstract::The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.1.330-333.1984
更新日期:1984-01-01 00:00:00
abstract::The syrB gene is required for syringomycin production by Pseudomonas syringae pv. syringae and full virulence during plant pathogenesis. Strain B3AR132 containing a syrB::lacZ fusion was used to detect transcriptional activation of the syrB gene in syringomycin minimal medium by plant metabolites with signal activity....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5784-5792.1991
更新日期:1991-09-01 00:00:00
abstract::FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00074-17
更新日期:2017-04-25 00:00:00
abstract::A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.238-246.1980
更新日期:1980-10-01 00:00:00
abstract::The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7379-7384.1992
更新日期:1992-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.17.6187-6196.2005
更新日期:2005-09-01 00:00:00
abstract::We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1731-1736.1987
更新日期:1987-04-01 00:00:00
abstract::The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.8074-8082.2004
更新日期:2004-12-01 00:00:00
abstract::Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1386-1394.1979
更新日期:1979-03-01 00:00:00
abstract::Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7378-7389.2004
更新日期:2004-11-01 00:00:00
abstract::The expression of the pur operon, which encodes enzymes of the purine biosynthetic pathway in Bacillus subtilis, is subject to control by the purR gene product (PurR) and phosphoribosylpyrophosphate. This control is also exerted on the purA and purR genes. A consensus sequence for the binding of PurR, named the PurBox...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.21.6175-6183.2001
更新日期:2001-11-01 00:00:00
abstract::Billen, Daniel (University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Tex.), and Roger Hewitt. Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J. Bacteriol. 92:609-617. 1966.-A study has been made of the subsequent replicative ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.3.609-617.1966
更新日期:1966-09-01 00:00:00
abstract::Twelve mutants of Escherichia coli K-12 have been isolated which carry chromosomal mutations that exhibit pleiotropic effects on the expression of F factor tra cistrons. F pilus synthesis, deoxyribonucleic acid transfer, and surface exclusion are all inhibited. Six of the mutants carry sfrA mutations, and six carry sf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.730-737.1979
更新日期:1979-09-01 00:00:00
abstract::Maintaining cell envelope integrity is critical for bacterial survival, including bacteria living in a complex and dynamic environment such as the human oral cavity. Streptococcus mutans, a major etiological agent of dental caries, uses two-component signal transduction systems (TCSTSs) to monitor and respond to vario...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01563-08
更新日期:2009-05-01 00:00:00
abstract::Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00839-08
更新日期:2008-11-01 00:00:00
abstract::The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00476-07
更新日期:2007-07-01 00:00:00