Conservative duplication of spindle poles during meiosis in Saccharomyces cerevisiae.

Abstract:

:During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores instead of four and that these spores are always nonsisters. In wild-type strains, Mpc70p localizes to all four spindle pole bodies, whereas in MPC70/mpc70 strains Mpc70p localizes to only two of the four spindle pole bodies, and these are always nonsisters. Our data can be explained by conservative spindle pole body distribution in which the two newly synthesized meiosis II spindle pole bodies of MPC70/mpc70 strains lack Mpc70p.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wesp A,Prinz S,Fink GR

doi

10.1128/JB.183.7.2372-2375.2001

keywords:

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

2372-5

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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