Abstract:
:During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores instead of four and that these spores are always nonsisters. In wild-type strains, Mpc70p localizes to all four spindle pole bodies, whereas in MPC70/mpc70 strains Mpc70p localizes to only two of the four spindle pole bodies, and these are always nonsisters. Our data can be explained by conservative spindle pole body distribution in which the two newly synthesized meiosis II spindle pole bodies of MPC70/mpc70 strains lack Mpc70p.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Wesp A,Prinz S,Fink GRdoi
10.1128/JB.183.7.2372-2375.2001keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
2372-5issue
7eissn
0021-9193issn
1098-5530journal_volume
183pub_type
杂志文章abstract::Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.3.500-506.1962
更新日期:1962-03-01 00:00:00
abstract::An NADPH-specific disulfide reductase that is active with bis-gamma-glutamylcystine has been purified 1,900-fold from Halobacterium halobium to yield a homogeneous preparation of the enzyme. Purification of this novel reductase, designated bis-gamma-glutamylcystine reductase (GCR), and purification of halobacterial di...
journal_title:Journal of bacteriology
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doi:10.1128/jb.170.8.3459-3467.1988
更新日期:1988-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00818-12
更新日期:2012-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6968-6975.2003
更新日期:2003-12-01 00:00:00
abstract::Genetic studies have shown that the 53-kDa (Exo53) and 49-kDa (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid homology, Exo53 has been shown to express ADP-ribosyltransferase activity at abou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.5.1609-1613.1997
更新日期:1997-03-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.96.3.629-638.1968
更新日期:1968-09-01 00:00:00
abstract::A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2017-09-05 00:00:00
abstract::It has been reported that the accumulation of K+ by Streptococcus faecalis is mediated by a transport system which required both ATP and the proton motive force (Bakker and Harold, J. Biol. Chem. 255:433-440, 1980). My results indicate that S. faecalis has a second transport system for K+. The features of this system ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.506-511.1982
更新日期:1982-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1147-1157.2004
更新日期:2004-02-01 00:00:00
abstract::Members of the pPT23A plasmid family of Pseudomonas syringae play an important role in the interaction of this bacterial pathogen with host plants. Complete sequence analysis of several pPT23A family plasmids (PFPs) has provided a glimpse of the gene content and virulence function of these plasmids. We constructed a m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.6.2113-2126.2005
更新日期:2005-03-01 00:00:00
abstract::Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1467-1471.1983
更新日期:1983-06-01 00:00:00
abstract::Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00071-12
更新日期:2012-06-01 00:00:00
abstract::Silicibacter sp. strain TM1040, a member of the Roseobacter clade, forms a symbiosis with unicellular phytoplankton, which is inextricably linked to the biphasic "swim or stick" lifestyle of the bacteria. Mutations in flaC bias the population toward the motile phase. Renewed examination of the FlaC(-) strain (HG1016) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01777-12
更新日期:2013-02-01 00:00:00
abstract::Sneathia amnii is a poorly characterized emerging pathogen that has been implicated in amnionitis and urethritis. We found that S. amnii damages fetal membranes, and we identified and purified a cytotoxic exotoxin that lyses human red blood cells and damages cells from fetal membranes. The gene appears to be cotranscr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00162-20
更新日期:2020-06-09 00:00:00
abstract::In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and Bacillus subtilisStreptomyces is a bacterial genus that is characte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00597-19
更新日期:2020-01-15 00:00:00
abstract::Carbon catabolite control protein A (CcpA) is the main regulator involved in carbon catabolite repression in gram-positive bacteria. Time series gene expression analyses of Lactococcus lactis MG1363 and L. lactis MG1363DeltaccpA using DNA microarrays were used to define the CcpA regulon of L. lactis. Based on a compar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01013-06
更新日期:2007-02-01 00:00:00
abstract::RNase D is a 3'-exoribonuclease whose in vitro specificity has suggested that it is involved in the processing of tRNA precursors. Its in vivo role has remained unclear, however, because mutant cells devoid of the enzyme display no defect in growth or tRNA processing. To learn more about the structure and function of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.522-527.1988
更新日期:1988-02-01 00:00:00
abstract::Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-bindin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.5145-5148.1992
更新日期:1992-08-01 00:00:00
abstract::Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.546-558.1968
更新日期:1968-02-01 00:00:00
abstract::Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1430-1438.1986
更新日期:1986-12-01 00:00:00
abstract::Lipoprotein biogenesis in Gram-negative bacteria occurs by a conserved pathway, each step of which is considered essential. In contrast to this model, LoVullo and colleagues demonstrate that the N-acyl transferase Lnt is not required in Francisella tularensis or Neisseria gonorrhoeae. This suggests the existence of a ...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00051-15
更新日期:2015-05-01 00:00:00
abstract::The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.4746-4754.1999
更新日期:1999-08-01 00:00:00
abstract::Between 59 and 60 min on the Escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. About 24 kilobase pairs downstream of the 14 repeats, a similar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3553-3556.1989
更新日期:1989-06-01 00:00:00
abstract::Citrate lyase, the key enzyme of anaerobic citrate catabolism, could not be deleted from Salmonella typhimurium. The only class of mutants found had a mode of covalent regulation that strongly resembled the Escherichia coli system: citrate lyase was only active, i.e., acetylated, when a cosubstrate was present. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.546-549.1983
更新日期:1983-01-01 00:00:00
abstract::By using a method that labels sulfhydryl-containing proteins in situ, we have detected a major outer membrane protein of Neisseria gonorrhoeae at 41 kDa. A protein of this molecular mass has not previously been shown to be a major outer membrane protein in gonococci. In addition, a minor protein rich in cysteinyl resi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.1173-1175.1993
更新日期:1993-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00490-09
更新日期:2009-09-01 00:00:00
abstract::Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1697-1705.1967
更新日期:1967-11-01 00:00:00
abstract::Different preparations of the methylreductase were tested in a simplified methylcoenzyme M methylreductase assay with artificial electron donors under a nitrogen atmosphere. ATP and Mg2+ stimulated the reaction. Tris(2,2'-bipyridine)ruthenium (II), chromous chloride, chromous acetate, titanium III citrate, 2,8-diamino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2711-2715.1988
更新日期:1988-06-01 00:00:00
abstract::Enteroaggregative Escherichia coli (EAggEC) has been associated with persistent pediatric diarrhea in the developing world, yet the pathogenetic mechanisms of EAggEC infection are unknown. Our previous data have suggested that aggregative adherence of some EAggEC strains to HEp-2 cells is mediated by flexible, bundle-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4691-4699.1994
更新日期:1994-08-01 00:00:00