Macromolecule synthesis in yeast spheroplasts.

abstract：:The valyl-transfer ribonucleic acid (tRNA) synthetase of Escherichia coli strain NP2907, previously described as having an elevated K(m) for adenosine triphosphate and reduced stability in vitro compared to the wild type, was found to be conditionally thermolabile in vivo. The rate of inactivation of this enzyme at a ...

journal_title：Journal of bacteriology

authors： Anderson JJ,Neidhardt FC

更新日期：1972-01-01 00:00:00

abstract：:Current mouse models for evaluating the efficacy of live oral cholera vaccines (OCVs) have important limitations. Conventionally raised adult mice are resistant to intestinal colonization by Vibrio cholerae, but germfree mice can be colonized and have been used to study OCV immunogenicity. However, germfree animals ha...

journal_title：Journal of bacteriology

authors： Fakoya B,Sit B,Waldor MK

更新日期：2020-11-19 00:00:00

abstract：:The deletion of a gene coding for a histidine kinase (sll0750, Hik8) in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 resulted in a conditional lethal phenotype with a pleiotropic effect on the expression of genes involved in glucose metabolism. This mutant had comparable doubling times to wild type...

journal_title：Journal of bacteriology

authors： Singh AK,Sherman LA

更新日期：2005-04-01 00:00:00

abstract：:The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

journal_title：Journal of bacteriology

authors： Shioi JI,Matsuura S,Imae Y

更新日期：1980-12-01 00:00:00

abstract：:The Bacillus subtilis sbo-alb operon contains sboA, the structural gene for the bacteriocin subtilosin, and the alb genes required for subtilosin production. Transcription from the sbo-alb promoter is highly induced by oxygen limitation. The transcriptional regulation of the sbo-alb operon is under dual control involv...

journal_title：Journal of bacteriology

authors： Nakano MM,Zheng G,Zuber P

更新日期：2000-06-01 00:00:00

abstract：:A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lacti...

journal_title：Journal of bacteriology

authors： Holo H,Nilssen O,Nes IF

更新日期：1991-06-01 00:00:00

abstract：:Cells colicinogenic for the colicin plasmids E1 or E2 (Col E1 and Col E2, respectively) were selected for a loss of colicin production after infection with bacteriophage Mu. Extrachromosomal deoxyribonucleic acid that was larger than the original colicin plasmids was found in such cells. A small insertion mutant in Co...

journal_title：Journal of bacteriology

authors： Inselburg J

更新日期：1974-08-01 00:00:00

abstract：:Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...

journal_title：Journal of bacteriology

authors： Reiner AM

更新日期：1969-03-01 00:00:00

abstract：:The first three enzymes of the pentachlorophenol (PCP) degradation pathway in Sphingobium chlorophenolicum (formerly Sphingomonas chlorophenolica) ATCC 39723 have been characterized, and the corresponding genes, pcpA, pcpB, and pcpC, have been individually cloned and sequenced. To search for new genes involved in PCP ...

journal_title：Journal of bacteriology

authors： Cai M,Xun L

更新日期：2002-09-01 00:00:00

abstract：:The weak organic acid sorbic acid is a commonly used food preservative, as it inhibits the growth of bacteria, yeasts, and molds. We have used genome-wide transcriptional profiling of Bacillus subtilis cells during mild sorbic acid stress to reveal the growth-inhibitory activity of this preservative and to identify po...

journal_title：Journal of bacteriology

authors： Ter Beek A,Keijser BJ,Boorsma A,Zakrzewska A,Orij R,Smits GJ,Brul S

更新日期：2008-03-01 00:00:00

abstract：:Analysis of chlorobenzene-degrading transconjugants of Pseudomonas putida F1 which had acquired the genes for chlorocatechol degradation (clc) from Pseudomonas sp. strain B13 revealed that the clc gene cluster was present on a 105-kb amplifiable genetic element (named the clc element). In one such transconjugant, P. p...

journal_title：Journal of bacteriology

authors： Ravatn R,Studer S,Springael D,Zehnder AJ,van der Meer JR

更新日期：1998-09-01 00:00:00

abstract：:The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...

journal_title：Journal of bacteriology

authors： Nakahara H,Silver S,Miki T,Rownd RH

更新日期：1979-10-01 00:00:00

abstract：:Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...

journal_title：Journal of bacteriology

authors： Langridge P,Morita RY

更新日期：1966-08-01 00:00:00

abstract：:The occurrence of class II restriction endonucleases (enzymes that both recognize and cleave a specific nucleotide sequence in deoxyribonucleic acid [DNA] in Staphylococcus aureus has been investigated by analysis of crude extracts obtained from different propagating strains of the International Phage Typing System. O...

journal_title：Journal of bacteriology

authors： Stobberingh EE,Schiphof R,Sussenbach JS

更新日期：1977-08-01 00:00:00

abstract：:With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...

journal_title：Journal of bacteriology

authors： Roberts I,Mountford R,High N,Bitter-Suermann D,Jann K,Timmis K,Boulnois G

更新日期：1986-12-01 00:00:00

abstract：:DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37 degrees C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open r...

journal_title：Journal of bacteriology

authors： Motin VL,Georgescu AM,Fitch JP,Gu PP,Nelson DO,Mabery SL,Garnham JB,Sokhansanj BA,Ott LL,Coleman MA,Elliott JM,Kegelmeyer LM,Wyrobek AJ,Slezak TR,Brubaker RR,Garcia E

更新日期：2004-09-01 00:00:00

abstract：:Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...

journal_title：Journal of bacteriology

authors： Brambl R

更新日期：1975-06-01 00:00:00

abstract：:In this study, we demonstrated that the surface-expressed enolase from diarrheal isolate SSU of Aeromonas hydrophila bound to human plasminogen and facilitated the latter's tissue-type plasminogen activator-mediated activation to plasmin. The bacterial surface-bound plasmin was more resistant to the action of its spec...

journal_title：Journal of bacteriology

authors： Sha J,Erova TE,Alyea RA,Wang S,Olano JP,Pancholi V,Chopra AK

更新日期：2009-05-01 00:00:00

abstract：:Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to thi...

journal_title：Journal of bacteriology

authors： Sherlock O,Dobrindt U,Jensen JB,Munk Vejborg R,Klemm P

更新日期：2006-03-01 00:00:00

abstract：:The exit of glutamate from Escherichia coli K-12 cells preloaded with the radioactive amino acid and its relation to the reaction of entry were studied. Experiments with cells preloaded to different intracellular concentrations of radioactive glutamate confirmed our earlier conclusion that glutamate exit was a first-o...

journal_title：Journal of bacteriology

authors： Halpern YS,Barash H,Druck K

更新日期：1973-01-01 00:00:00

abstract：:Dienelactone hydrolases have previously been shown to play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. Recently, the enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelact...

journal_title：Journal of bacteriology

authors： Schlömann M,Ngai KL,Ornston LN,Knackmuss HJ

更新日期：1993-05-01 00:00:00

abstract：:Type III secretion systems (T3SSs) secrete needle components, pore-forming translocators, and the translocated effectors. In part, effector recognition by a T3SS involves their N-terminal amino acids and their 5' mRNA. To investigate whether similar molecular constraints influence translocator secretion, we scrutinize...

journal_title：Journal of bacteriology

authors： Amer AA,Åhlund MK,Bröms JE,Forsberg Å,Francis MS

更新日期：2011-12-01 00:00:00

abstract：:phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from...

journal_title：Journal of bacteriology

authors： Pajunen MI,Kiljunen SJ,Söderholm ME,Skurnik M

更新日期：2001-03-01 00:00:00

abstract：:Genome sequences from the prolific Roseobacter clade in the Alphaproteobacteria are beginning to reveal the genetic basis for the diverse lifestyles of these organisms. Here we present the genome sequences of Oceanicola granulosus HTCC2516(T) and Oceanicola batsensis HTCC2597(T), two marine Roseobacter species isolate...

journal_title：Journal of bacteriology

authors： Thrash JC,Cho JC,Vergin KL,Giovannoni SJ

更新日期：2010-07-01 00:00:00

abstract：:Pili are functionally expressed during the predivisional and swarmer stages of the Caulobacter crescentus differentiation cycle. They appear on the developing swarmer pole and at the same cellular location as flagella and the phiCbK receptor sites. Pili disappear when the swarmer cell differentiates into a stalked cel...

journal_title：Journal of bacteriology

authors： Lagenaur C,Agabian N

更新日期：1977-07-01 00:00:00

abstract：:We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...

journal_title：Journal of bacteriology

authors： Pacelli LZ,Edmiston SH,Mount DW

更新日期：1979-01-01 00:00:00

abstract：:A series of R (rough) Salmonella minnesota mutants with rfb, rfe, and rfa mutations leading to various defects in the biosynthesis of cell wall lipopolysaccharide was analyzed as to their enterobacterial common antigen (CA) content. All mutants that had functional rfe genes were CA(+) as is the wild-type parent. This ...

journal_title：Journal of bacteriology

authors： Mäkelä PH,Mayer H,Whang HY,Neter E

更新日期：1974-09-01 00:00:00

abstract：:Degenerate oligonucleotides based on the published Escherichia coli glutamate decarboxylase (GAD) protein sequence were used in a polymerase chain reaction to generate a DNA probe for the E. coli GAD structural gene. Southern blots showed that there were two cross-hybridizing GAD genes, and both of these were cloned a...

journal_title：Journal of bacteriology

authors： Smith DK,Kassam T,Singh B,Elliott JF

更新日期：1992-09-01 00:00:00

abstract：:Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA. ...

journal_title：Journal of bacteriology

authors： Sancar A,Hack AM,Rupp WD

更新日期：1979-01-01 00:00:00

abstract：:DivIVA is involved in Bacillus subtilis cell division and is located at the cell poles. Previous experiments suggested that the cell division proteins FtsZ and PBP 2B are required for polar targeting of DivIVA. By using outgrowing spores, we show that DivIVA accumulates at the cell poles independent of the presence of...

journal_title：Journal of bacteriology

authors： Hamoen LW,Errington J

更新日期：2003-01-01 00:00:00