Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli.

abstract：:Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be d...

journal_title：Journal of bacteriology

authors： GERHARDT P,BLACK SH

更新日期：1961-11-01 00:00:00

abstract：:RNase D is a 3'-exoribonuclease whose in vitro specificity has suggested that it is involved in the processing of tRNA precursors. Its in vivo role has remained unclear, however, because mutant cells devoid of the enzyme display no defect in growth or tRNA processing. To learn more about the structure and function of ...

journal_title：Journal of bacteriology

authors： Zhang JR,Deutscher MP

更新日期：1988-02-01 00:00:00

abstract：:We described previously a class of mutations (phenotypic designation R2(-)) in Staphylococcus aureus that were unlinked to penicillinase genes but nevertheless imposed constitutive synthesis of this enzyme upon wild-type plasmids. The plasmids affected by these mutations have been found to possess the same maintenance...

journal_title：Journal of bacteriology

authors： Cohen S,Vernon EG,Sweeney HM

更新日期：1970-09-01 00:00:00

abstract：:A human isolate of Salmonella enteritidis which displayed strong pellicle formation during static broth culture and mannose-sensitive hemagglutination produced fimbriae which were morphologically indistinguishable from type 1 fimbriae of members of the family Enterobacteriaceae. Fimbrin was purified to homogeneity, an...

journal_title：Journal of bacteriology

authors： Feutrier J,Kay WW,Trust TJ

更新日期：1986-10-01 00:00:00

abstract：:The bacterioferritin (BFR) of Escherichia coli K-12 is an iron-storage hemoprotein, previously identified as cytochrome b1. The bacterioferritin gene (bfr) has been cloned, sequenced, and located in the E. coli linkage map. Initially a gene fusion encoding a BFR-lambda hybrid protein (Mr 21,000) was detected by immuno...

journal_title：Journal of bacteriology

authors： Andrews SC,Harrison PM,Guest JR

更新日期：1989-07-01 00:00:00

abstract：:D-Glucose dehydrogenase is a pyrroloquinoline quinone-dependent primary dehydrogenase linked to the respiratory chain of a wide variety of bacteria. The enzyme exists in the membranes of Escherichia coli, mainly as an apoenzyme which can be activated by the addition of pyrroloquinoline quinone and magnesium. Thus, mem...

journal_title：Journal of bacteriology

authors： Matsushita K,Nonobe M,Shinagawa E,Adachi O,Ameyama M

更新日期：1987-01-01 00:00:00

abstract：:Transcriptional fusions to Salmonella typhimurium chromosomal genes were constructed by integration of a suicide fusion vector into the chromosome by homologous recombination with random cloned chromosomal fragments. We describe here a transductional method using the generalized transducing phage of S. typhimurium, P2...

journal_title：Journal of bacteriology

authors： Mahan MJ,Slauch JM,Mekalanos JJ

更新日期：1993-11-01 00:00:00

abstract：:Proteus mirabilis, an agent of urinary tract infection, expresses at least four fimbrial types. Among these are the MR/P (mannose-resistant/Proteus-like) fimbriae. MrpA, the structural subunit, is optimally expressed at 37 degrees C in Luria broth cultured statically for 48 h by each of seven strains examined. Genes e...

journal_title：Journal of bacteriology

authors： Bahrani FK,Mobley HL

更新日期：1994-06-01 00:00:00

abstract：:Derivatives of Pseudomonas sp. B13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the TOL plasmid of Pseudomonas putida mt-2 were studied. The utilization of these substrates, a property not shared by the parent strains, was shown to dep...

journal_title：Journal of bacteriology

authors： Reineke W,Knackmuss HJ

更新日期：1980-05-01 00:00:00

abstract：:Transcriptomic, metabolomic, physiological, and computational modeling approaches were integrated to gain insight into the mechanisms of antibiotic tolerance in an in vitro biofilm system. Pseudomonas aeruginosa biofilms were grown in drip flow reactors on a medium composed to mimic the exudate from a chronic wound. A...

journal_title：Journal of bacteriology

authors： Stewart PS,White B,Boegli L,Hamerly T,Williamson KS,Franklin MJ,Bothner B,James GA,Fisher S,Vital-Lopez FG,Wallqvist A

更新日期：2019-10-21 00:00:00

abstract：:A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...

journal_title：Journal of bacteriology

authors： Malki S,Saimmaime I,De Luca G,Rousset M,Dermoun Z,Belaich JP

更新日期：1995-05-01 00:00:00

abstract：:A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...

journal_title：Journal of bacteriology

authors： Hurlbert JC,Preston JF 3rd

更新日期：2001-03-01 00:00:00

abstract：:Heating at 55 C causes the intracellular degradation of ribosomes of Staphylococcus aureus MF-31. The 30S subunit of heated cells appears to be selectively attacked. Analysis of ribonucleic acid (RNA) showed that 16S RNA was destroyed and that the secondary structure of 23S RNA was altered. ...

journal_title：Journal of bacteriology

authors： Rosenthal LJ,Iandolo JJ

更新日期：1970-09-01 00:00:00

abstract：:The sdiA gene codes for a protein that regulates expression of the ftsQAZ cluster of essential cell division genes of Escherichia coli. SdiA up-regulates the ftsQ2p promoter that initiates transcription into the ftsQAZ cluster. In this paper, we report that expression of sdiA is itself regulated by a factor that is re...

journal_title：Journal of bacteriology

authors： García-Lara J,Shang LH,Rothfield LI

更新日期：1996-05-01 00:00:00

abstract：:The diphtheria toxin repressor (DtxR) uses Fe(2+) as a corepressor and inhibits transcription from iron-regulated promoters (IRPs) in Corynebacterium diphtheriae. A new IRP, designated IRP6, was cloned from C. diphtheriae by a SELEX-like procedure. DtxR bound to IRP6 in vitro only in the presence of appropriate divale...

journal_title：Journal of bacteriology

authors： Qian Y,Lee JH,Holmes RK

更新日期：2002-09-01 00:00:00

abstract：:Saccharomyces cerevisiae cells, grown on a fermentable carbon source, display two growth phases before they enter the stationary phase: a rapid phase (log phase) followed by a slow phase. It was previously shown that a subunit of the yeast RNA polymerase II, RPB4, positively affects the activity of the enzyme in post-...

journal_title：Journal of bacteriology

authors： Choder M

更新日期：1993-10-01 00:00:00

abstract：:Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...

journal_title：Journal of bacteriology

authors： STEVENS JG,GROMAN NB

更新日期：1964-02-01 00:00:00

abstract：:Previously, we characterized a pathway necessary for the processing of NAD+ and for uptake of nicotinamide riboside (NR) in Haemophilus influenzae. Here we report on the role of NadR, which is essential for NAD+ utilization in this organism. Different NadR variants with a deleted ribonucleotide kinase domain or with a...

journal_title：Journal of bacteriology

authors： Merdanovic M,Sauer E,Reidl J

更新日期：2005-07-01 00:00:00

abstract：:Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the pro...

journal_title：Journal of bacteriology

authors： Sheehan SM,Switzer RL

更新日期：1990-01-01 00:00:00

abstract：:Spectinomycin-resistant (Spcr) mutants of Escherichia coli were isolated from nutrient agar plates containing 20% sucrose and 100 mug of spectinomycin per ml. About one-third of the Spcr mutants thus obtained were sucrose dependent (Sucd) and were classified into two types: I, those unable to grow on sucrose-free medi...

journal_title：Journal of bacteriology

authors： Miyoshi Y,Yamagata H

更新日期：1976-01-01 00:00:00

abstract：:Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

journal_title：Journal of bacteriology

authors： Calhoun DH,Feary TW

更新日期：1969-01-01 00:00:00

abstract：:Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...

journal_title：Journal of bacteriology

authors： Lascelles J,Hatch TP

更新日期：1969-05-01 00:00:00

abstract：:Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...

journal_title：Journal of bacteriology

authors： Kriszt B,Táncsics A,Cserháti M,Tóth Á,Nagy I,Horváth B,Nagy I,Tamura T,Kukolya J,Szoboszlay S

更新日期：2012-03-01 00:00:00

abstract：:Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...

journal_title：Journal of bacteriology

authors： Marketon MM,González JE

更新日期：2002-07-01 00:00:00

abstract：:The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-s...

journal_title：Journal of bacteriology

authors： Tong J,Wetmur JG

更新日期：1996-05-01 00:00:00

abstract：:In a previous study, we showed that in bacteroids, transcription of the inducible nod genes does not occur and expression of nodD is decreased by 65% (H. R. M. Schlaman, B. Horvath, E. Vijgenboom, R.J.H. Okker, and B. J. J. Lugtenberg, J. Bacteriol. 173:4277-4287, 1991). In the present study, we show, using gel retard...

journal_title：Journal of bacteriology

authors： Schlaman HR,Lugtenberg BJ,Okker RJ

更新日期：1992-10-01 00:00:00

abstract：:Jones, Lois M. (University of Wisconsin, Madison), C. R. McDuff, and J. B. Wilson. Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state. J. Bacteriol. 83:860-866. 1962.-In the course of examining a number of Brucella cultures with a brucellaphage, it was observed t...

journal_title：Journal of bacteriology

authors： JONES LM,McDUFF CR,WILSON JB

更新日期：1962-04-01 00:00:00

abstract：:The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

journal_title：Journal of bacteriology

authors： Amikam D,Benziman M

更新日期：1989-12-01 00:00:00

abstract：:Heterocyst differentiation is orchestrated by the N control transcriptional regulator NtcA and the differentiation-specific factor HetR. In Anabaena sp. strain PCC 7120, the devBCA operon is expressed from two different promoters activated upon N stepdown. The distal devB promoter (transcription start point [TSP] loca...

journal_title：Journal of bacteriology

authors： Camargo S,Valladares A,Flores E,Herrero A

更新日期：2012-06-01 00:00:00

abstract：:The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...

journal_title：Journal of bacteriology

authors： Lohrke SM,Nechaev S,Yang H,Severinov K,Jin SJ

更新日期：1999-08-01 00:00:00