Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria.


:The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-stranded DNA-dependent ATPase activity at their temperature optima (> or = 70 degrees C). In the absence of ATP, T. thermophilus RuvB protein bound to linear double-stranded DNA with a preference for the ends. Addition of ATP or gamma-S-ATP destabilized the T. thermophilus RuvB-DNA complexes. Both thermostable RuvB proteins displayed helicase activity on supercoiled DNA. Expression of thermostable T. thermophilus RuvB protein in the E. coli ruvB recG mutant strain N3395 partially complemented the UV-sensitive phenotype, suggesting that T. thermophilus RuvB protein has a function similar to that of E. coli RuvB in vivo.


J Bacteriol


Journal of bacteriology


Tong J,Wetmur JG




Has Abstract


1996-05-01 00:00:00












  • UDP-glucuronic acid decarboxylases of Bacteroides fragilis and their prevalence in bacteria.

    abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Coyne MJ,Fletcher CM,Reinap B,Comstock LE

    更新日期:2011-10-01 00:00:00

  • Recombination in the ompA gene but not the omcB gene of Chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism.

    abstract::Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Millman KL,Tavaré S,Dean D

    更新日期:2001-10-01 00:00:00

  • MECHANISM OF ACTION OF THE TOXIN OF BACILLUS ANTHRACIS II. : Alkaline Phosphatasemia Produced by Culture Filtrates of Various Bacilli.

    abstract::Slein, Milton W. (Fort Detrick, Frederick, Md.) and Gerald F. Logan, Jr. Mechanism of action of the toxin of Bacillus anthracis. II. Alkaline phosphatasemia produced by culture filtrates of various bacilli. J. Bacteriol. 83:359-369. 1962.-A factor which produces hyperphosphatasemia after intravenous injection into ani...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Slein MW,Logan GF

    更新日期:1962-02-01 00:00:00

  • Allantoate transport in Saccharomyces cerevisiae.

    abstract::Allantoate uptake appears to be mediated by an energy-dependent active transport system with an apparent Michaelis constant of about 50 microM. Cells were able to accumulate allantoate to greater than 3,000 times the extracellular concentration. The rate of accumulation was maximum at pH 5.7 to 5.8. The energy source ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Turoscy V,Cooper TG

    更新日期:1979-12-01 00:00:00

  • Freeze-etching of the cell envelope of an Acinetobacter species which carries a regular array of surface subunits.

    abstract::Freeze-etching was applied to preparations, with and without glycerol, of Acinetobacter sp. strain MJT/F5/199A, consisting of intact cells after normal growth or after incubation with chloramphenicol, spheroplasts, and isolated cell walls and outer membranes. Etched preparations show that a regular array of subunits f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sleytr UB,Thornley MJ

    更新日期:1973-12-01 00:00:00

  • Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids.

    abstract::Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies reveal that the plasmids coding for the production of heat stable and heat labile enteroxtoxins of Escherichia coli, regardless of their origin, have a majority of their polynucleotide sequences in common, but are not related in any significant way to t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: So M,Crosa JH,Falkow S

    更新日期:1975-01-01 00:00:00

  • Oxidative metabolism in Pediococcus pentosaceus. II. Factors controlling the formation of oxidative activities.

    abstract::Dobrogosz, Walter J. (The Pennsylvania State University, University Park) and Robert W. Stone. Oxidative metabolism in Pediococcus pentosaceus. II. Factors controlling the formation of oxidative activities. J. Bacteriol. 84:724-729. 1962.-Studies were conducted on some factors which regulate the formation of oxidative...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-10-01 00:00:00

  • Global mutational analysis of NtrC-like activators in Myxococcus xanthus: identifying activator mutants defective for motility and fruiting body development.

    abstract::The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

    更新日期:2003-10-01 00:00:00

  • Staphylococcal penicillinase. I. Inhibition and stimulation of activity.

    abstract::Saz, Arthur K. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Dolores L. Lowery, and Leah J. Jackson. Staphylococcal penicillinase. I. Inhibition and stimulation of activity. J. Bacteriol. 82:298-304. 1961.-The penicillinase extracted from a penicillin-resistant strain of Staphylococcus aureus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1961-08-01 00:00:00

  • A new family of integral membrane proteins involved in transport of aromatic amino acids in Escherichia coli.

    abstract::The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sarsero JP,Wookey PJ,Gollnick P,Yanofsky C,Pittard AJ

    更新日期:1991-05-01 00:00:00

  • Requirement of NifX and other nif proteins for in vitro biosynthesis of the iron-molybdenum cofactor of nitrogenase.

    abstract::The iron-molybdenum cofactor (FeMo-co) of nitrogenase contains molybdenum, iron, sulfur, and homocitrate in a ratio of 1:7:9:1. In vitro synthesis of FeMo-co has been established, and the reaction requires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabolic product of Ni...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shah VK,Rangaraj P,Chatterjee R,Allen RM,Roll JT,Roberts GP,Ludden PW

    更新日期:1999-05-01 00:00:00

  • Sociality in Escherichia coli: Enterochelin Is a Private Good at Low Cell Density and Can Be Shared at High Cell Density.

    abstract:UNLABELLED:Many bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of productio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Scholz RL,Greenberg EP

    更新日期:2015-07-01 00:00:00

  • Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pat

    abstract::The glmU gene product of Escherichia coli was recently identified as the N-acetylglucosamine-1-phosphate uridyltransferase activity which catalyzes the formation of UDP-N-acetylglucosamine, an essential precursor for cell wall peptidoglycan and lipopolysaccharide biosyntheses (D. Mengin-Lecreulx and J. van Heijenoort,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mengin-Lecreulx D,van Heijenoort J

    更新日期:1994-09-01 00:00:00

  • Analytical studies on regeneration of protoplasts of Geotrichum candidum by quantitative thin-layer-agar plating.

    abstract::A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fukui K,Sagara Y,Yoshida N,Matsuoka T

    更新日期:1969-04-01 00:00:00

  • Role of EscP (Orf16) in injectisome biogenesis and regulation of type III protein secretion in enteropathogenic Escherichia coli.

    abstract::Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Monjarás Feria J,García-Gómez E,Espinosa N,Minamino T,Namba K,González-Pedrajo B

    更新日期:2012-11-01 00:00:00

  • Genome sequence of the agar-degrading marine bacterium Alteromonadaceae sp. strain G7.

    abstract::Here, we present the high-quality draft genome sequence of the agar-degrading marine gammaproteobacterium Alteromonadaceae sp. strain G7, which was isolated from coastal seawater to be utilized as a bioresource for production of agar-derived biofuels. The 3.91-Mb genome contains a number of genes encoding algal polysa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kwak MJ,Song JY,Kim BK,Chi WJ,Kwon SK,Choi S,Chang YK,Hong SK,Kim JF

    更新日期:2012-12-01 00:00:00

  • mgpS, a complex regulatory locus involved in the transcriptional control of the puc and puf operons in Rhodobacter sphaeroides 2.4.1.

    abstract::A new method has been developed in order to select mutants showing decreased puc operon transcription in Rhodobacter sphaeroides 2.4.1. A transcriptional fusion of a promoterless fragment derived from the sacB gene, encoding the levansucrase from Bacillus subtilis, to the upstream regulatory region of the puc operon h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sabaty M,Kaplan S

    更新日期:1996-01-01 00:00:00

  • Complementarity of Bacillus subtilis 16S rRNA with sites of antibiotic-dependent ribosome stalling in cat and erm leaders.

    abstract::Inducible cat and erm genes are regulated by translational attenuation. In this regulatory model, gene activation results from chloramphenicol- or erythromycin-dependent stalling of a ribosome at a precise site in the leader region of cat or erm transcripts. The stalled ribosome is believed to destabilize a downstream...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rogers EJ,Ambulos NP Jr,Lovett PS

    更新日期:1990-11-01 00:00:00

  • Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome Reveals a Compartmentalization Bias toward the Bacterial Surface.

    abstract::The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dowdell AS,Murphy MD,Azodi C,Swanson SK,Florens L,Chen S,Zückert WR

    更新日期:2017-02-28 00:00:00

  • Regulation of amino acid transport in Escherichia coli by transcription termination factor rho.

    abstract::Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Quay SC,Oxender DL

    更新日期:1977-06-01 00:00:00

  • The C-terminus of substrates is critical but not sufficient for their degradation by the Pseudomonas aeruginosa CtpA protease.

    abstract::Bacterial carboxyl-terminal processing proteases (CTPs) are widely conserved and have been linked to important processes including signal transduction, cell wall metabolism, and virulence. However, the features that target proteins for CTP-dependent cleavage are unclear. Studies of the Escherichia coli CTP Prc suggest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chung S,Darwin AJ

    更新日期:2020-06-01 00:00:00

  • Second system for potassium transport in Streptococcus faecalis.

    abstract::It has been reported that the accumulation of K+ by Streptococcus faecalis is mediated by a transport system which required both ATP and the proton motive force (Bakker and Harold, J. Biol. Chem. 255:433-440, 1980). My results indicate that S. faecalis has a second transport system for K+. The features of this system ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kobayashi H

    更新日期:1982-05-01 00:00:00

  • Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component flavin-diffusible monooxygenase.

    abstract::Pseudomonas sp. VLB120 uses styrene as a sole source of carbon and energy. The first step in this metabolic pathway is catalyzed by an oxygenase (StyA) and a NADH-flavin oxidoreductase (StyB). Both components have been isolated from wild-type Pseudomonas strain VLB120 as well as from recombinant Escherichia coli. StyA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Otto K,Hofstetter K,Röthlisberger M,Witholt B,Schmid A

    更新日期:2004-08-01 00:00:00

  • The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin.

    abstract::The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies with F-pilin polypeptides in inner membrane preparations from various F mutant strains. It was known that JEL92 recognizes ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Moore D,Hamilton CM,Maneewannakul K,Mintz Y,Frost LS,Ippen-Ihler K

    更新日期:1993-03-01 00:00:00

  • Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2.

    abstract::The complete nucleotide sequence of the linear plasmid pBD2 from Rhodococcus erythropolis BD2 comprises 210,205 bp. Sequence analyses of pBD2 revealed 212 putative open reading frames (ORFs), 97 of which had an annotatable function. These ORFs could be assigned to six functional groups: plasmid replication and mainten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stecker C,Johann A,Herzberg C,Averhoff B,Gottschalk G

    更新日期:2003-09-01 00:00:00

  • EnvZ functions through OmpR to control porin gene expression in Escherichia coli K-12.

    abstract::The regulatory proteins OmpR and EnvZ are both required to activate expression of the genes for the major outer membrane porin proteins, OmpF and OmpC, of Escherichia coli K-12. Here we show that OmpR, under certain conditions, could activate porin expression in the complete absence of EnvZ. In addition, the pleiotrop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Slauch JM,Garrett S,Jackson DE,Silhavy TJ

    更新日期:1988-01-01 00:00:00

  • Cobalt targets multiple metabolic processes in Salmonella enterica.

    abstract::Cobalt is essential for growth of Salmonella enterica and other organisms, yet this metal can be toxic when present in excess. Wild-type Salmonella exhibits several metabolic defects when grown in the presence of cobalt, some of which generate visible growth consequences. Work herein identifies sulfur assimilation, ir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thorgersen MP,Downs DM

    更新日期:2007-11-01 00:00:00

  • The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation.

    abstract::Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sawers G,Hesslinger C,Muller N,Kaiser M

    更新日期:1998-07-01 00:00:00

  • A distant upstream site involved in the negative regulation of the Escherichia coli ompF gene.

    abstract::The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Huang KJ,Schieberl JL,Igo MM

    更新日期:1994-03-01 00:00:00


    abstract::von Graevenitz, Alexander (Yale University, New Haven, Conn.). Genetic transfer of streptomycin and aminopropanolstreptomycin resistance. J. Bacteriol. 88:960-964. 1964.-Transduction and conjugation experiments with Staphylococcus aureus and Escherichia coli suggest that resistance to streptomycin (S) and aminopropano...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: VONGRAEVENITZ A

    更新日期:1964-10-01 00:00:00