Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12.

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:The Firmicutes Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes convert thiosulfate, forming sulfur globules inside and outside cells. X-ray absorption near-edge structure analysis revealed that the sulfur consisted mainly of sulfur chains with organic end groups similar to sulfur formed ...

journal_title：Journal of bacteriology

authors： Lee YJ,Prange A,Lichtenberg H,Rohde M,Dashti M,Wiegel J

更新日期：2007-10-01 00:00:00

abstract：:Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...

journal_title：Journal of bacteriology

authors： Kingan SL,Ensign JC

更新日期：1968-09-01 00:00:00

abstract：:Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, ...

journal_title：Journal of bacteriology

authors： Bodenmiller DM,Spiro S

更新日期：2006-02-01 00:00:00

abstract：:The ftsZ gene of Escherichia coli, which lies in a cluster of cell division genes at 2 min on the genetic map, codes for a protein which is thought to play a key role in triggering cell division. Using an ftsZ::lacZ operon fusion, we have studied the transcription of the ftsZ gene under conditions in which cell divisi...

journal_title：Journal of bacteriology

authors： Robin A,Joseleau-Petit D,D'Ari R

更新日期：1990-03-01 00:00:00

abstract：:The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...

journal_title：Journal of bacteriology

authors： Kasahara M,Makino K,Amemura M,Nakata A,Shinagawa H

更新日期：1991-01-01 00:00:00

abstract：:The tar and tap loci of Escherichia coli encode methyl-accepting inner membrane proteins that mediate chemotactic responses to aspartate and maltose or to dipeptides. These genes lie adjacent to each other in the same orientation on the chromosome and have extensive sequence homology throughout the C-terminal portions...

journal_title：Journal of bacteriology

authors： Slocum MK,Halden NF,Parkinson JS

更新日期：1987-07-01 00:00:00

abstract：:Accumulation of intracellular allantoin and allantoate is mediated by two distinct active transport systems in Saccharomyces cerevisiae. Allantoin transport (DAL4 gene) is inducible, while allantoate uptake is constitutive (it occurs at full levels in the absence of any allantoate-related compounds from the culture me...

journal_title：Journal of bacteriology

authors： Chisholm VT,Lea HZ,Rai R,Cooper TG

更新日期：1987-04-01 00:00:00

abstract：:A specialized transducing phage lambda carrying the structural gene for the OmpF protein, an outer membrane matrix protein, was isolated. The phage carries the 20.5--21-min region of the Escherichia coli K-12 chromosome and carries asnS, ompF, and aspC genes. ...

journal_title：Journal of bacteriology

authors： Mutoh N,Nagasawa T,Mizushima S

更新日期：1981-02-01 00:00:00

abstract：:In order to study the Salmonella typhimurium cobalamin biosynthetic pathway, the S. typhimurium cob operon was isolated and cloned into Escherichia coli. This approach has given the new host of the cob operon the ability to make cobalamins de novo, an ability that had probably been lost by this organism. In total, 20 ...

journal_title：Journal of bacteriology

authors： Raux E,Lanois A,Levillayer F,Warren MJ,Brody E,Rambach A,Thermes C

更新日期：1996-02-01 00:00:00

abstract：:The Staphylococcus aureus cidABC and lrgAB operons have been shown to regulate murein hydrolase activity and affect antibiotic tolerance. The cid operon enhances murein hydrolase activity and antibiotic sensitivity, whereas the lrg operon inhibits these processes. Based on these findings and the structural similaritie...

journal_title：Journal of bacteriology

authors： Yang SJ,Rice KC,Brown RJ,Patton TG,Liou LE,Park YH,Bayles KW

更新日期：2005-09-01 00:00:00

abstract：:Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

journal_title：Journal of bacteriology

authors： Rawls KS,Martin JH,Maupin-Furlow JA

更新日期：2011-09-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00

abstract：:The fumarate reductase enzyme complex allows Escherichia coli to grow anaerobically with fumarate as a terminal electron acceptor for oxidative phosphorylation when the preferred compounds oxygen and nitrate are not available. We used the pKO promoter test vectors to identify a single promoter for the frdABCD genes wh...

journal_title：Journal of bacteriology

authors： Jones HM,Gunsalus RP

更新日期：1985-12-01 00:00:00

abstract：:Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxan...

journal_title：Journal of bacteriology

authors： Garzón A,Li J,Flores A,Casadesus J,Stewart V

更新日期：1992-10-01 00:00:00

abstract：:Widely studied Salmonella enterica serovar Typhimurium strains ATCC 14028s and SL1344 harbor a cryptic ST64B prophage unable to produce infectious virions. We found that coculturing either strain with an isogenic sibling lacking the prophage leads to the appearance of active forms of the virus. Active phage originates...

journal_title：Journal of bacteriology

authors： Figueroa-Bossi N,Bossi L

更新日期：2004-06-01 00:00:00

abstract：:We have recently established that the facultative phototrophic bacterium Rhodobacter sphaeroides, like the closely related Rhodobacter capsulatus species, contains both the previously characterized mobile electron carrier cytochrome c2 (cyt c2) and the more recently discovered membrane-anchored cyt cy. However, R. sph...

journal_title：Journal of bacteriology

authors： Daldal F,Mandaci S,Winterstein C,Myllykallio H,Duyck K,Zannoni D

更新日期：2001-03-01 00:00:00

abstract：:Das, S. K. (University of Calcutta, Calcutta, India) and G. C. Chatterjee. Pyrithiamine adaptation of Staphylococcus aureus. I. Adaptation and carbohydrate utilization. J. Bacteriol. 83:1251-1259. 1962.-Staphylococcus aureus has been adapted to pyrithiamine, a thiamine analogue; as a result of this adaptation, the col...

journal_title：Journal of bacteriology

authors： DAS SK,CHATTERJEE GC

更新日期：1962-06-01 00:00:00

abstract：:Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

journal_title：Journal of bacteriology

authors： Short KA,Blakemore RP

更新日期：1986-08-01 00:00:00

abstract：:Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been p...

journal_title：Journal of bacteriology

authors： Brush GS,Bhatnagar SK,Bessman MJ

更新日期：1990-06-01 00:00:00

abstract：:Escherichia coli K-12 strain CU1008 cannot use L-serine as the sole carbon source, but it could use L-serine as an auxiliary carbon source with glucose, L-alanine, or pyruvate and could derive energy from L-serine to support oxygen uptake. CU1008 grew with L-serine if it was also provided with glycine and leucine. The...

journal_title：Journal of bacteriology

authors： Newman EB,Walker C

更新日期：1982-08-01 00:00:00

abstract：:Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...

journal_title：Journal of bacteriology

authors： Viale AM,Kobayashi H,Akazawa T

更新日期：1989-05-01 00:00:00

abstract：:We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity ...

journal_title：Journal of bacteriology

authors： Delany I,Pacheco AB,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-08-01 00:00:00

abstract：:Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

journal_title：Journal of bacteriology

authors： Madigan MT,Brock TD

更新日期：1975-05-01 00:00:00

abstract：:A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...

journal_title：Journal of bacteriology

authors： Malki S,Saimmaime I,De Luca G,Rousset M,Dermoun Z,Belaich JP

更新日期：1995-05-01 00:00:00

abstract：:Expression of the hurIR bhuRSTUV heme utilization locus in Bordetella bronchiseptica is coordinately controlled by the global iron-dependent regulator Fur and the extracytoplasmic function sigma factor HurI. Activation of HurI requires transduction of a heme-dependent signal via HurI, HurR, and BhuR, a three-component...

journal_title：Journal of bacteriology

authors： King-Lyons ND,Smith KF,Connell TD

更新日期：2007-09-01 00:00:00

abstract：:Capsulated Haemophilus influenzae type b and two spontaneous mutants (classes I and II variants) were characterized by transmission and scanning electron microscopy. When cells were treated with type b-specific antiserum prior to manipulations for electron microscopy, sectioned capsulated cells had electron-dense, fib...

journal_title：Journal of bacteriology

authors： Doern GV,Buckmire FL

更新日期：1976-07-01 00:00:00

abstract：:A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...

journal_title：Journal of bacteriology

authors： Neway JO,Switzer RL

更新日期：1983-08-01 00:00:00

abstract：:The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter o...

journal_title：Journal of bacteriology

authors： Ferguson GP,Roop RM 2nd,Walker GC

更新日期：2002-10-01 00:00:00

abstract：:Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

journal_title：Journal of bacteriology

authors： Stern ML,Phillips AW,Gottlieb AJ

更新日期：1976-02-01 00:00:00