Physical properties of L-asparaginase from Serratia marcescens.

abstract：:A new osmotically inducible gene in Escherichia coli, osmY, was induced 8- to 10-fold by hyperosmotic stress and 2- to 3-fold by growth in complex medium. The osmY gene product is a periplasmic protein which migrates with an apparent molecular mass of 22 kDa on sodium dodecyl sulfate-polyacrylamide gels. A genetic fus...

journal_title：Journal of bacteriology

authors： Yim HH,Villarejo M

更新日期：1992-06-01 00:00:00

abstract：:Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF all...

journal_title：Journal of bacteriology

authors： Pooley HM,Abellan FX,Karamata D

更新日期：1992-01-01 00:00:00

abstract：:Isolation of six strains of Escherichia coli K-12 carrying mutations affecting the citrate-dependent iron uptake system is described. Genetic analysis of these mutants showed that the mutation affecting the citrate system are cluster together at about min 6 on the E. coli chromosome. ...

journal_title：Journal of bacteriology

authors： Woodrow GC,Langman L,Young IG,Gibson F

更新日期：1978-03-01 00:00:00

abstract：:FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

journal_title：Journal of bacteriology

authors： Grantcharova N,Lustig U,Flärdh K

更新日期：2005-05-01 00:00:00

abstract：:The extent of change in cytoplasmic proteins which accompanies yeast-to-mycelium morphogenesis of Candida albicans was analyzed by two-dimensional gel electrophoresis. Pure cultures of yeasts and true hyphae (i.e., without concomitant production of pseudohyphae) were grown in a synthetic low-sulfate medium. The two st...

journal_title：Journal of bacteriology

authors： Manning M,Mitchell TG

更新日期：1980-10-01 00:00:00

abstract：:Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...

journal_title：Journal of bacteriology

authors： Brunskill EW,Bayles KW

更新日期：1996-02-01 00:00:00

abstract：:A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

journal_title：Journal of bacteriology

authors： Rastogi VK,Watson RJ

更新日期：1991-05-01 00:00:00

abstract：:Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...

journal_title：Journal of bacteriology

authors： Xu D,Yang C,Kaplan HB

更新日期：1998-12-01 00:00:00

abstract：:Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutant...

journal_title：Journal of bacteriology

authors： Rock CO,Park HW,Jackowski S

更新日期：2003-06-01 00:00:00

abstract：:Different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium Desulfovibrio desulfuricans (Norway strain) have been studied. Two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. This ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six resid...

journal_title：Journal of bacteriology

authors： Bruschi M,Hatchikian CE,Golovleva LA,Gall JL

更新日期：1977-01-01 00:00:00

abstract：:Studies with methionineless strains of Escherichia coli WWU and Bacillus megaterium KM have shown that selenomethionine only partially satisfies their methionine requirement. ...

journal_title：Journal of bacteriology

authors： Wu M,Wachsman JT

更新日期：1970-12-01 00:00:00

abstract：:The carbon monoxide (CO) dehydrogenase activity band from Clostridium pasteurianum was shown to contain nickel by in situ activity staining of polyacrylamide gels. However, the majority of the nickel in cell extracts was found to electrophorese independently of CO dehydrogenase. Comparative analysis with Clostridium t...

journal_title：Journal of bacteriology

authors： Drake HL

更新日期：1982-02-01 00:00:00

abstract：:Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...

journal_title：Journal of bacteriology

authors： Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

更新日期：1998-06-01 00:00:00

abstract：:Transcriptional expression of the puc operon in Rhodobacter sphaeroides 2.4.1 is dependent on the partial pressure of oxygen. By using transcriptional fusions in trans of a promoterless fragment derived from the aminoglycoside-3'-phosphotransferase gene of Tn903 to puc operon-specific DNA containing a 629-bp 5' cis-ac...

journal_title：Journal of bacteriology

authors： Lee JK,Kaplan S

更新日期：1992-02-01 00:00:00

abstract：:DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

journal_title：Journal of bacteriology

authors： Asai T,Kogoma T

更新日期：1994-11-01 00:00:00

abstract：:Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular enterotoxin concentration roughly paralleled but followed by 2.5 to 5 hr the increase in number of heat-resistant spores. The increase ...

journal_title：Journal of bacteriology

authors： Duncan CL

更新日期：1973-02-01 00:00:00

abstract：:The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

journal_title：Journal of bacteriology

authors： Kim SW,Kim CY,Benisek WF,Choi KY

更新日期：1994-11-01 00:00:00

abstract：:By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...

journal_title：Journal of bacteriology

authors： Vermeulen CA,Venema G

更新日期：1974-05-01 00:00:00

abstract：:We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...

journal_title：Journal of bacteriology

authors： Letellier L,Labedan B

更新日期：1984-03-01 00:00:00

abstract：:Population density-dependent expression of luminescence in Vibrio fischeri is controlled by the autoinducer N-3-oxohexanoyl-L-homoserine lactone (autoinducer 1 [AI-1]), which via LuxR activates transcription of the lux operon (luxICDABEG, encoding the putative autoinducer synthase [LuxI] and the luminescence enzymes)....

journal_title：Journal of bacteriology

authors： Kuo A,Callahan SM,Dunlap PV

更新日期：1996-02-01 00:00:00

abstract：:The first three enzymes of the pentachlorophenol (PCP) degradation pathway in Sphingobium chlorophenolicum (formerly Sphingomonas chlorophenolica) ATCC 39723 have been characterized, and the corresponding genes, pcpA, pcpB, and pcpC, have been individually cloned and sequenced. To search for new genes involved in PCP ...

journal_title：Journal of bacteriology

authors： Cai M,Xun L

更新日期：2002-09-01 00:00:00

abstract：:An inducible phosphoenolpyruvate:fructose phosphotransferase system has been detected in Azospirillum brasilense, which requires a minimum of two components of the crude extracts for activity: (i) a soluble fraction (enzyme I) and (ii) a membrane fraction (enzyme II). The uninduced cells neither show any uptake of fru...

journal_title：Journal of bacteriology

authors： Gupta KD,Ghosh S

更新日期：1984-12-01 00:00:00

abstract：:A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...

journal_title：Journal of bacteriology

authors： Young CS,Beatty JT

更新日期：1998-09-01 00:00:00

abstract：:Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...

journal_title：Journal of bacteriology

authors： Lindgren PB,Peet RC,Panopoulos NJ

更新日期：1986-11-01 00:00:00

abstract：:For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...

journal_title：Journal of bacteriology

authors： Das S,Kellermann E,Hollenberg CP

更新日期：1984-06-01 00:00:00

abstract：:Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) muta...

journal_title：Journal of bacteriology

authors： Wilson KJ,Anjaiah V,Nambiar PT,Ausubel FM

更新日期：1987-05-01 00:00:00

abstract：:A fusion between the pCloDF13-derived bacteriocin release protein and beta-lactamase was constructed to investigate the subcellular localization and posttranslational modification of the bacteriocin release protein in Escherichia coli. The signal sequence and 25 of the 28 amino acid residues of the mature bacteriocin ...

journal_title：Journal of bacteriology

authors： Luirink J,Watanabe T,Wu HC,Stegehuis F,de Graaf FK,Oudega B

更新日期：1987-05-01 00:00:00

abstract：:The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a reg...

journal_title：Journal of bacteriology

authors： Marcus M,Halpern YS

更新日期：1969-03-01 00:00:00

abstract：:Genome comparisons have demonstrated that dramatic genetic change often underlies the emergence of new bacterial pathogens. Evolutionary analysis of Escherichia coli O157:H7, a pathogen that has emerged as a worldwide public health threat in the past two decades, has posited that this toxin-producing pathogen evolved ...

journal_title：Journal of bacteriology

authors： Wick LM,Qi W,Lacher DW,Whittam TS

更新日期：2005-03-01 00:00:00

abstract：:Physiological studies of a mutant of Escherichia coli lacking the three lytic transglycosylases Slt70, MltA, and MltB revealed that interference with murein turnover can prevent AmpC beta-lactamase induction. The triple mutant, although growing normally, shows a dramatically reduced rate of murein turnover. Despite th...

journal_title：Journal of bacteriology

authors： Kraft AR,Prabhu J,Ursinus A,Höltje JV

更新日期：1999-12-01 00:00:00