CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase, which is involved in the synthesis of the major wall teichoic acid in Bacillus subtilis 168, is encoded by tagF (rodC).

Abstract:

:Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF alleles. In vitro, thermosensitivity of CGPTase strongly suggests that the structural gene for this enzyme is tagF. We discuss apparent discrepancies between biochemical evidence favoring a membrane location for TagF and a previous report that suggested a cytoplasmic location based on sequence analysis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pooley HM,Abellan FX,Karamata D

doi

10.1128/jb.174.2.646-649.1992

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

646-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

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