Growth and glucose repression are controlled by glucose transport in Saccharomyces cerevisiae cells containing only one glucose transporter.

Abstract:

:A set of Saccharomyces cerevisiae strains with variable expression of only the high-affinity Hxt7 glucose transporter was constructed by partial deletion of the HXT7 promoter in vitro and integration of the gene at various copy numbers into the genome of an hxt1-7 gal2 deletion strain. The glucose transport capacity increased in strains with higher levels of HXT7 expression. The consequences for various physiological properties of varying the glucose transport capacity were examined. The control coefficient of glucose transport with respect to growth rate was 0.54. At high extracellular glucose concentrations, both invertase activity and the rate of oxidative glucose metabolism increased manyfold with decreasing glucose transport capacity, which is indicative of release from glucose repression. These results suggest that the intracellular glucose concentration produces the signal for glucose repression.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ye L,Kruckeberg AL,Berden JA,van Dam K

doi

10.1128/JB.181.15.4673-4675.1999

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

4673-5

issue

15

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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