Abstract:
:Broad-host-range plasmids and cloning vectors were conjugatively transferred to Acetobacter xylinum. One of the plasmids, RP4::Mu cts61, was used for the insertion of Tn1 into the 16-, 44-, and 64-kilobase-pair plasmids of A. xylinum. The Tn1-labeled plasmids could be mobilized by a helper plasmid. Many of the Tn1 insertions affected the copy number of the plasmids.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Valla S,Coucheron DH,Kjosbakken Jdoi
10.1128/jb.165.1.336-339.1986subject
Has Abstractpub_date
1986-01-01 00:00:00pages
336-9issue
1eissn
0021-9193issn
1098-5530journal_volume
165pub_type
杂志文章abstract::A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.820-825.1984
更新日期:1984-06-01 00:00:00
abstract::Salmonella enterica encodes a type III secretion system within a pathogenicity island located at centisome 63 that is essential for virulence. All type III secretion systems require the function of a family of low-molecular-weight proteins that aid the secretion process by acting as partitioning factors and/or secreti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2262-2268.2000
更新日期:2000-04-01 00:00:00
abstract::Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.15.4700-4706.1991
更新日期:1991-08-01 00:00:00
abstract::Photobacterium leiognathi ATCC 25521 (the type strain and light-organ symbiont of ponyfish) is one of the few bacteria that produces a copper-zinc superoxide dismutase, termed bacteriocuprein. We enzymologically and immunologically characterized the bacteriocuprein superoxide dismutases in sonicates from the type stra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.393-398.1986
更新日期:1986-02-01 00:00:00
abstract::Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.900-910.1978
更新日期:1978-09-01 00:00:00
abstract::Members of the pPT23A plasmid family of Pseudomonas syringae play an important role in the interaction of this bacterial pathogen with host plants. Complete sequence analysis of several pPT23A family plasmids (PFPs) has provided a glimpse of the gene content and virulence function of these plasmids. We constructed a m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.6.2113-2126.2005
更新日期:2005-03-01 00:00:00
abstract::Integrons facilitate the evolution of complex phenotypes by physical and transcriptional linkage of genes. They can be categorized as chromosomal integrons (CIs) or mobile resistance integrons (MRIs). The significance of MRIs for the problem of multiple antibiotic resistance is well established. CIs are more widesprea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00229-08
更新日期:2008-07-01 00:00:00
abstract::A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2628-2636.1995
更新日期:1995-05-01 00:00:00
abstract::Gellan gum is a widely used commercial material, available in many different forms. Its economic importance has led to studies into the biosynthesis of exopolysaccharide gellan gum, which is industrially prepared in high yields using Sphingomonas elodea ATCC 31461. Glucose-1-phosphate uridylyltransferase mediates the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00277-07
更新日期:2007-06-01 00:00:00
abstract::Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1090-1100.1974
更新日期:1974-06-01 00:00:00
abstract::In recent work, we described the excision of a large genomic region from Enterococcus faecium D344R in which the sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00859-06
更新日期:2007-05-01 00:00:00
abstract::Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.1972-1982.2004
更新日期:2004-04-01 00:00:00
abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3448-3452.1998
更新日期:1998-07-01 00:00:00
abstract::The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00560-12
更新日期:2012-09-01 00:00:00
abstract::We have found, using a newly developed genetic method, a protein (named Cnu, for oriC-binding nucleoid-associated) that binds to a specific 26-base-pair sequence (named cnb) in the origin of replication of Escherichia coli, oriC. Cnu is composed of 71 amino acids (8.4 kDa) and shows extensive amino acid identity to a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.20.6998-7008.2005
更新日期:2005-10-01 00:00:00
abstract::D-[alpha-14C]]glucosyl phosphorylpolyprenol ([ 14C]Glc-P-prenol) was formed from UDP-D-[14C]glucose in each of the membrane systems obtained from Bacillus coagulans AHU 1631 and AHU 1634 and two Bacillus megaterium strains. Membranes of these B. coagulans strains, which possess beta-D-glucosyl branches on the repeatin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2835-2841.1989
更新日期:1989-05-01 00:00:00
abstract::The expression of cell cycle events in Caulobacter crescentus CB13 has been shown to be associated with regulation of carbohydrate utilization. Growth on lactose and galactose depends on induction of specific enzymes. Prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.3.951-959.1977
更新日期:1977-09-01 00:00:00
abstract::Arylamine N-acetyltransferase activity has been described in various bacterial species. Bacterial N-acetyltransferases, including those from bacteria of the gut flora, may be involved in the metabolism of xenobiotics, thereby exerting physiopathological effects. We characterized these enzymes further by steady-state k...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.11.3417-3427.2001
更新日期:2001-06-01 00:00:00
abstract::It is thought that in the gram-negative soil bacterium Sinorhizobium meliloti the protein ExoP is involved in biosynthesis of the acidic exopolysaccharide succinoglycan (EPS I). The amounts and compositions of EPS I produced by mutants expressing ExoP proteins characterized by specific amino acid substitutions in the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.17.5163-5170.2001
更新日期:2001-09-01 00:00:00
abstract::Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.19.6683-6690.2005
更新日期:2005-10-01 00:00:00
abstract::A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1429-1433.1983
更新日期:1983-09-01 00:00:00
abstract::The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5460-5469.2005
更新日期:2005-08-01 00:00:00
abstract::The transcriptional regulatory protein Rgg coordinates amino acid catabolism and virulence factor expression in Streptococcus pyogenes. We used a proteomic approach to compare cytoplasmic proteins isolated from S. pyogenes wild-type strain NZ131 (serotype M49) to proteins isolated from an rgg mutant strain during the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7091-7099.2004
更新日期:2004-11-01 00:00:00
abstract::A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.5911-5917.2001
更新日期:2001-10-01 00:00:00
abstract::Mild alkaline extraction of native poly-beta-hydroxybutyrate (PHB) granules results in the solubilization of a protein fraction. Both the solubilized protein fraction and the extracted granules are essentially devoid of PHB synthetase activity unless recombined. The protein fraction has been separated by chromatograph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.2.782-789.1971
更新日期:1971-11-01 00:00:00
abstract::Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outsi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3096-3104.1999
更新日期:1999-05-01 00:00:00
abstract::Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.460-463.1984
更新日期:1984-05-01 00:00:00
abstract::In Escherichia coli, pyrimidine-mediated regulation of upp expression occurs by UTP-sensitive selection of alternative transcriptional start sites, which produces transcripts that differ in the ability to be elongated. The upp initially transcribed region contains the sequence GATTTTTTTTG (nontemplate strand). Initiat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.221-228.2001
更新日期:2001-01-01 00:00:00
abstract::MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2124-2131.1999
更新日期:1999-04-01 00:00:00
abstract::Cells of the photosynthetic bacterium Rhodospirillum rubrum cultivated anaerobically in light show phototaxis. The behavior of individual cells in response to the phenomenon is reversal(s) of the swimming direction when the intensity of the light available to them abruptly decreases. The tactic response was inhibited ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.34-41.1977
更新日期:1977-07-01 00:00:00