Abstract:
:Integrons facilitate the evolution of complex phenotypes by physical and transcriptional linkage of genes. They can be categorized as chromosomal integrons (CIs) or mobile resistance integrons (MRIs). The significance of MRIs for the problem of multiple antibiotic resistance is well established. CIs are more widespread, but their only demonstrated significance is as a reservoir of gene cassettes for MRIs. In characterizing CIs associated with Pseudomonas, we discovered a subfamily of insertion sequences, termed the IS1111-attC group, that insert into the recombination sites of gene cassettes (attC site) by site-specific recombination. IS1111-attC elements appear to have recently spread from Pseudomonas species to clinical class 1 integrons. Such elements are expected to significantly impact integrons. To explore this further, we examined CIs in 24 strains representing multiple levels of evolutionary divergence within the genus Pseudomonas. Cassette arrays frequently had a degenerated "footprint" of an IS1111-attC group element at their terminus and in three cases were occupied by multiple functional IS1111-attC elements. Within Pseudomonas spp. the IS-integron interaction appears to follow an evolutionarily rapid cycle of infection, expansion, and extinction. The final outcome is extinction of the IS element and modification of the right-hand boundary of the integron. This system represents an unusual example of convergent evolution whereby heterologous families of site-specific recombinases of distinct genetic elements have adopted the same target site. The interactions described here represent a model for evolutionary processes that offer insights to a number of aspects of the biology of integrons and other mosaic genetic elements.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Tetu SG,Holmes AJdoi
10.1128/JB.00229-08subject
Has Abstractpub_date
2008-07-01 00:00:00pages
4959-70issue
14eissn
0021-9193issn
1098-5530pii
JB.00229-08journal_volume
190pub_type
杂志文章abstract::The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.1.161-166.1979
更新日期:1979-10-01 00:00:00
abstract::Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3256-3261.1999
更新日期:1999-05-01 00:00:00
abstract::The glycan chains in peptidoglycan or murein are cross-linked by transpeptidation of the peptide side chains. To assess the fraction of side chains involved in cross-bridges, distinction has been made between cross-linkage and cross-linking. The first expression refers to the situation in unlabeled (or fully labeled) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.6.2028-2031.1992
更新日期:1992-03-01 00:00:00
abstract::During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.179-185.1992
更新日期:1992-01-01 00:00:00
abstract::The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.193-200.1979
更新日期:1979-04-01 00:00:00
abstract::Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1700-1704.1964
更新日期:1964-12-01 00:00:00
abstract::Enterococcus faecalis ATCC 29212, a vancomycin-sensitive strain, has been extensively used as a representative control strain for clinical and laboratory experiments. Here we report the draft genome and annotation of this strain, containing 3,027,060 bp, with a G+C content of 37.2% in 126 contigs (≥ 500 bp). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01423-12
更新日期:2012-11-01 00:00:00
abstract::The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.2.623-628.1985
更新日期:1985-08-01 00:00:00
abstract::Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are distinguished from all other mycobacteria and from one another by the presence of highly antigenic glycolipids, the glycopepti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6991-6997.1991
更新日期:1991-11-01 00:00:00
abstract::We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced allosteric change of cAMP receptor protein that is necessary for activation of the protein. Currently, we have isolated int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1417-1422.1988
更新日期:1988-04-01 00:00:00
abstract::An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.3.806-814.1971
更新日期:1971-09-01 00:00:00
abstract::By controlled addition of galactose to synchronized galactose-limited Saccharomyces cerevisiae cultures, the growth rate could be regulated while external conditions were kept constant. By using this method, the G1 phase duration was modulated and expression of cell cycle-regulated genes was investigated. The expressi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6560-6565.1997
更新日期:1997-11-01 00:00:00
abstract::Macrocysts have been found among strains of Dictyostelium rosarium Raper and Cavender (K. B. Raper and J. C. Cavender, J. Elisha Mitchell Sci. Soc. 84:31-47, 1968). Strains that exhibit mating capabilities could be classified into one of three mating types: A1, A2, or A3. Each mating type was self-incompatible but cro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.1049-1053.1981
更新日期:1981-09-01 00:00:00
abstract::Conjugative transfer and replacement of hundreds of kilobases of a bacterial chromosome can occur in vitro, but replacements in nature are either an order of magnitude smaller or involve the movement of mobile genetic elements. We discovered that two lineages of Staphylococcus aureus, including a pandemic methicillin-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1060-1064.2004
更新日期:2004-02-01 00:00:00
abstract::To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2326-2328.2000
更新日期:2000-04-01 00:00:00
abstract::The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.15.4533-4539.1999
更新日期:1999-08-01 00:00:00
abstract::The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.18.5971-5977.1992
更新日期:1992-09-01 00:00:00
abstract::trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and transl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4272-4278.1995
更新日期:1995-08-01 00:00:00
abstract::The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.411-415.1980
更新日期:1980-07-01 00:00:00
abstract::The allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. The implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.763-769.1970
更新日期:1970-03-01 00:00:00
abstract::Cells of Haemophilus influenzae strain Rd competent for genetic transformation irreversibly bound approximately five molecular fragments of H. influenzae deoxyribonucleic acid (DNA) per cell; under identical conditions, DNA derived from Escherichia coli B was not taken up (<1 molecule per 50 cells). Similarly, DNA fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.369-373.1974
更新日期:1974-05-01 00:00:00
abstract::We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3496-3499.1990
更新日期:1990-06-01 00:00:00
abstract::Exogenously supplied long-chain fatty acids have been shown to markedly alleviate the inhibition of phototrophic growth of cultures of Rhodopseudomonas sphaeroides caused by the antibiotic cerulenin. Monounsaturated and polyunsaturated C18 fatty acids were most effective in relieving growth inhibition mediated by ceru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.782-790.1983
更新日期:1983-02-01 00:00:00
abstract::At early stages of sporulation, the spoIIA locus is transcribed as a tricistronic (1.7-kb) operon, coding for sigma F and for two proteins that modulate the activity of sigma F. The locus is transcribed as a longer (2.9-kb) transcript at the late stages of sporulation. We show here that the longer transcript contains ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.4885-4892.1992
更新日期:1992-08-01 00:00:00
abstract::The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.12.6764-6770.1989
更新日期:1989-12-01 00:00:00
abstract::The organization and transcriptional control of chromosomal cat genes (required for dissimilation of catechol by the beta-ketoadipate pathway) in the Pseudomonas putida biotype strain (ATCC 12633) are reported. Nucleotide sequence reveals that catR is separated by 135 bp from the divergently transcribed catBC,A; catC ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.2.401-412.1995
更新日期:1995-01-01 00:00:00
abstract::Based on its genome sequence, the pathway of beta-oxidative fatty acid degradation in Salmonella enterica serovar Typhimurium LT2 has been thought to be identical to the well-characterized Escherichia coli K-12 system. We report that wild-type strains of S. enterica grow on decanoic acid, whereas wild-type E. coli str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.2.599-608.2006
更新日期:2006-01-01 00:00:00
abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.808-820.1978
更新日期:1978-06-01 00:00:00
abstract::Auxotrophs of Acinetobacter calcoaceticus blocked in each reaction of the synthetic pathway from chorismic acid to tryptophan were obtained after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. One novel class was found to be blocked in both anthranilate and p-aminobenzoate synthesis; these mutants (trpG) require p-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.2.797-805.1972
更新日期:1972-11-01 00:00:00
abstract::A chemotaxis system for Pseudomonas aeruginosa was defined by using the method of Adler. Cells were attracted to compounds in the order ammonium chloride greater than amino acids greater than organic acids. Two sugars were assayed and elicited no response. Comparisons with other model systems are discussed. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.427-429.1978
更新日期:1978-01-01 00:00:00