Repression of nitrate reductase in Neurospora studied by using L-methionine-DL-sulfoximine and glutamine auxotroph gln-1b.

abstract：:Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...

journal_title：Journal of bacteriology

authors： Lam HM,Tancula E,Dempsey WB,Winkler ME

更新日期：1992-03-01 00:00:00

abstract：:tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

journal_title：Journal of bacteriology

authors： Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

更新日期：2019-04-09 00:00:00

abstract：:In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and Bacillus subtilisStreptomyces is a bacterial genus that is characte...

journal_title：Journal of bacteriology

authors： Łebkowski T,Wolański M,Ołdziej S,Flärdh K,Zakrzewska-Czerwińska J

更新日期：2020-01-15 00:00:00

abstract：:Besides producing thymine-requiring mutants (thy), trimethoprim (TMP) cured the mini-ColE1 replicon pML21 at an appreciable frequency. The cured Escherichia coli K-12 cells behaved like polA mutants by failing to support the stable maintenance of the ColE1 plasmid. The mini-F replicon pSC138, which was lacking all thr...

journal_title：Journal of bacteriology

authors： Mukhopadhyay P,Palchaudhuri S

更新日期：1982-05-01 00:00:00

abstract：:Vibrio cholerae hemolysin (HlyA) is a 65-kDa water-soluble pore-forming toxin that causes lysis of eukaryotic cells by destroying selective permeability of the plasma membrane bilayer. The HlyA monomer self-assembles on the target cell surface to the more stable beta-barrel amphipathic heptamer, which inserts into the...

journal_title：Journal of bacteriology

authors： Dutta S,Mazumdar B,Banerjee KK,Ghosh AN

更新日期：2010-01-01 00:00:00

abstract：:The trfA gene of the broad-host-range plasmid RK2 is essential for initiation of plasmid replication. Two related TrfA proteins of 43 and 32 kilodaltons (kDa) are produced by independent translation initiation at two start codons within the trfA open reading frame. These proteins were o overproduced in Escherichia col...

journal_title：Journal of bacteriology

authors： Durland RH,Helinski DR

更新日期：1990-07-01 00:00:00

abstract：:During the transition from a free-swimming, single-cell lifestyle to a sessile, multicellular state called a biofilm, bacteria produce and secrete an extracellular matrix comprised of nucleic acids, exopolysaccharides, and adhesion proteins. The Vibrio cholerae biofilm matrix contains three major protein components, R...

journal_title：Journal of bacteriology

authors： Giglio KM,Fong JC,Yildiz FH,Sondermann H

更新日期：2013-07-01 00:00:00

abstract：:Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...

journal_title：Journal of bacteriology

authors： Ebisu S,Tsuboi A,Takagi H,Naruse Y,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1990-03-01 00:00:00

abstract：:During meiosis and sporulation in Saccharomyces cerevisiae, the recessive genetic marker for cycloheximide resistance, believed to be due to an altered ribosomal protein (C. S. McLaughlin, p. 815-827, in M. Nomura et al., ed., Ribosomes, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.), is expressed as early a...

journal_title：Journal of bacteriology

authors： Emanuel JR,Magee PT

更新日期：1981-03-01 00:00:00

abstract：:Five Spirochaeta aurantia chemotaxis mutants were isolated. One mutant (the che-101 mutant) never reversed, one (the che-200 mutant) flexed predominantly, two (the che-300 and che-400-1 mutants) exhibited elevated reversal frequencies, and one (the che-400 mutant) exhibited chemotactically unstimulated behavior simila...

journal_title：Journal of bacteriology

authors： Fosnaugh K,Greenberg EP

更新日期：1989-01-01 00:00:00

abstract：:The cysteines of the Escherichia coli periplasmic enzyme alkaline phosphatase, which are involved in disulfide bonds in the native enzyme, were found to be fully reduced when the protein was retained in the cytoplasm. Under these circumstances the cysteines remained reduced for at least several minutes after the synth...

journal_title：Journal of bacteriology

authors： Derman AI,Beckwith J

更新日期：1991-12-01 00:00:00

abstract：:In gram-negative bacteria, a pathway for aerobic degradation of phenylacetic acid (PAA) that proceeds via phenylacetyl-coenzyme A (CoA) and hydrolytic ring fission plays a central role in the degradation of a range of aromatic compounds. In contrast, the PAA pathway and its role are not well characterized in gram-posi...

journal_title：Journal of bacteriology

authors： Navarro-Llorens JM,Patrauchan MA,Stewart GR,Davies JE,Eltis LD,Mohn WW

更新日期：2005-07-01 00:00:00

abstract：:The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...

journal_title：Journal of bacteriology

authors： Shattuck-Eidens DM,Kadner RJ

更新日期：1983-09-01 00:00:00

abstract：:The production of extracellular alpha-amylase and protease by protoplasts of Bacillus amyloliquefaciens has been achieved. The production of enzymically active protease was totally dependent on a high concentration of either Mg2+, Ca2+, or spermidine, but production of active alpha-amylase was not. This cation depende...

journal_title：Journal of bacteriology

authors： Sanders RL,May BK

更新日期：1975-09-01 00:00:00

abstract：:Remsen, C. C. (Syracuse University, Syracuse, N.Y.), D. G. Lundgren, and R. A. Slepecky. Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol. J. Bacteriol. 91:324-331. 1966.-The effect of phenethyl alcohol (PEA) upon the initial stages of sporulation in Bacillus...

journal_title：Journal of bacteriology

authors： Remsen CC,Lundgren DG,Slepecky RA

更新日期：1966-01-01 00:00:00

abstract：:Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

journal_title：Journal of bacteriology

authors： Gregory MA,Till R,Smith MC

更新日期：2003-09-01 00:00:00

abstract：:The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...

journal_title：Journal of bacteriology

authors： Koudelka AP,Hufnagel LA,Koudelka GB

更新日期：2004-11-01 00:00:00

abstract：:A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, resulting in the substitution of the residue alanine 262 by valine in motif...

journal_title：Journal of bacteriology

authors： Willison JC,Härtlein M,Leberman R

更新日期：1995-06-01 00:00:00

abstract：:A second cluster of genes encoding the E1 alpha, E1 beta, and E2 subunits of branched-chain alpha-keto acid dehydrogenase (BCDH), bkdFGH, has been cloned and characterized from Streptomyces avermitilis, the soil microorganism which produces anthelmintic avermectins. Open reading frame 1 (ORF1) (bkdF, encoding E1 alpha...

journal_title：Journal of bacteriology

authors： Denoya CD,Fedechko RW,Hafner EW,McArthur HA,Morgenstern MR,Skinner DD,Stutzman-Engwall K,Wax RG,Wernau WC

更新日期：1995-06-01 00:00:00

abstract：:Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

journal_title：Journal of bacteriology

authors： Goldman BS,Lin JT,Stewart V

更新日期：1994-08-01 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homolo...

journal_title：Journal of bacteriology

authors： Smith MD,Clewell DB

更新日期：1984-12-01 00:00:00

abstract：:For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...

journal_title：Journal of bacteriology

authors： Das S,Kellermann E,Hollenberg CP

更新日期：1984-06-01 00:00:00

abstract：:Fluctuations in osmolarity are one of the most prevalent stresses to which bacteria must adapt, both hypo- and hyperosmotic conditions. Most bacteria cope with high osmolarity by accumulating compatible solutes (osmolytes) in the cytoplasm to maintain the turgor pressure of the cell. Vibrio parahaemolyticus, a halophi...

journal_title：Journal of bacteriology

authors： Gregory GJ,Dutta A,Parashar V,Boyd EF

更新日期：2020-11-19 00:00:00

abstract：:Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis, with an emphasis on identifying small genes activated during spore formation. Nineteen transcription units were identified, 11 of which were shown to depend on one ...

journal_title：Journal of bacteriology

authors： Schmalisch M,Maiques E,Nikolov L,Camp AH,Chevreux B,Muffler A,Rodriguez S,Perkins J,Losick R

更新日期：2010-10-01 00:00:00

abstract：:The spoVM gene encodes a 26-amino-acid polypeptide that is essential for spore formation in Bacillus subtilis. A transposon insertion within the spoVM open reading frame has been shown to encode a chimeric protein which is biologically inactive and produces a phenotype identical to that of a deletion and insertion mut...

journal_title：Journal of bacteriology

authors： Cutting S,Anderson M,Lysenko E,Page A,Tomoyasu T,Tatematsu K,Tatsuta T,Kroos L,Ogura T

更新日期：1997-09-01 00:00:00

abstract：:We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...

journal_title：Journal of bacteriology

authors： London RE,Allen DL,Gabel SA,DeRose EF

更新日期：1999-06-01 00:00:00

abstract：:Goldman, Manuel (The University of Michigan, Ann Arbor), and Harold J. Blumenthal. Pathways of glucose catabolism in Bacillus cereus. J. Bacteriol. 87:377-386. 1964.-Estimates by a radiorespirometric method of the pathways of glucose catabolism of resting-cell suspensions of Bacillus cereus strain terminalis indicate ...

journal_title：Journal of bacteriology

authors： GOLDMAN M,BLUMENTHAL HJ

更新日期：1964-02-01 00:00:00

abstract：:In order to examine the potential role of bacterial collagenases in periodontal tissue destruction, we recently isolated a gene, prtC, from Porphyromonas gingivalis ATCC 53977, which expressed collagenase activity (N. Takahashi, T. Kato, and H. K. Kuramitsu, FEMS Microbiol. Lett. 84:135-138, 1991). The nucleotide sequ...

journal_title：Journal of bacteriology

authors： Kato T,Takahashi N,Kuramitsu HK

更新日期：1992-06-01 00:00:00

abstract：:Neisseria meningitidis can be the causative agent of meningitis or septicemia. This bacterium expresses type IV pili, which mediate a variety of functions, including autoagglutination, twitching motility, biofilm formation, adherence, and DNA uptake during transformation. The secretin PilQ supports type IV pilus extru...

journal_title：Journal of bacteriology

authors： Balasingham SV,Collins RF,Assalkhou R,Homberset H,Frye SA,Derrick JP,Tønjum T

更新日期：2007-08-01 00:00:00