Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm.

abstract：:Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...

journal_title：Journal of bacteriology

authors： Pérez-Martínez I,Zhao Y,Murillo J,Sundin GW,Ramos C

更新日期：2008-01-01 00:00:00

abstract：:The Caulobacter crescentus DNA adenine methyltransferase CcrM and its homologs in the alpha-Proteobacteria are essential for viability. CcrM is 34% identical to the yhdJ gene products of Escherichia coli and Salmonella enterica. This study provides evidence that the E. coli yhdJ gene encodes a DNA adenine methyltransf...

journal_title：Journal of bacteriology

authors： Broadbent SE,Balbontin R,Casadesus J,Marinus MG,van der Woude M

更新日期：2007-06-01 00:00:00

abstract：:The two highly homologous cbb operons of Alcaligenes eutrophus H16 that are located on the chromosome and on megaplasmid pHG1 contain genes encoding several enzymes of the Calvin carbon reduction cycle. Sequence analysis of a region from the promoter-distal part revealed two open reading frames, designated cbbT and cb...

journal_title：Journal of bacteriology

authors： Schäferjohann J,Yoo JG,Kusian B,Bowien B

更新日期：1993-11-01 00:00:00

abstract：:A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication...

journal_title：Journal of bacteriology

authors： Witkin EM,Roegner-Maniscalco V

更新日期：1992-06-01 00:00:00

abstract：:Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...

journal_title：Journal of bacteriology

authors： Wilson CR,Baldwin NJ

更新日期：1978-10-01 00:00:00

abstract：:Isolated cell walls of Staphylococcus aureus and Micrococcus lysodeikticus were found to expand and contract in response to changes in environmental pH and ionic strength. These volume changes, which could amount to as much as a doubling of wall dextran-impermeable volume, were related to changes in electrostatic inte...

journal_title：Journal of bacteriology

authors： Ou LT,Marquis RE

更新日期：1970-01-01 00:00:00

abstract：:Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer...

journal_title：Journal of bacteriology

authors： Shirai Y,Akiyama Y,Ito K

更新日期：1996-02-01 00:00:00

abstract：:The Escherichia coli structural gene for glutathione synthetase, gshB, was cloned into pBR322. Plasmids containing gshB were able to complement the glutathione requirement of a trxA gshB double mutant, and cells containing the plasmids were found to have elevated levels of glutathione synthetase. A mutant gshB allele ...

journal_title：Journal of bacteriology

authors： Daws T,Lim CJ,Fuchs JA

更新日期：1989-09-01 00:00:00

abstract：:Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells...

journal_title：Journal of bacteriology

authors： Chédin F,Dervyn R,Ehrlich SD,Noirot P

更新日期：1997-02-01 00:00:00

abstract：:Bacteriocins are ribosomally synthesized proteinaceous antibacterial peptides. They selectively interfere with the growth of other bacteria. The production and secretion of bacteriocins confer a distinct ecological advantage to the producer in competing against other bacteria that are present in the same ecological ni...

journal_title：Journal of bacteriology

authors： Dufour D,Barbour A,Chan Y,Cheng M,Rahman T,Thorburn M,Stewart C,Finer Y,Gong SG,Lévesque CM

更新日期：2020-05-27 00:00:00

abstract：:Transcription-coupled repair (TCR) is a cellular process by which some forms of DNA damage are repaired more rapidly from transcribed strands of active genes than from nontranscribed strands or the overall genome. In humans, the TCR coupling factor, CSB, plays a critical role in restoring transcription following both ...

journal_title：Journal of bacteriology

authors： Schalow BJ,Courcelle CT,Courcelle J

更新日期：2012-05-01 00:00:00

abstract：:A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. ...

journal_title：Journal of bacteriology

authors： Giddings TH,Cantino EC

更新日期：1974-11-01 00:00:00

abstract：:Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

journal_title：Journal of bacteriology

authors： Samhita L,Nanjundiah V,Varshney U

更新日期：2014-07-01 00:00:00

abstract：:Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linole...

journal_title：Journal of bacteriology

authors： Rabinowitch HD,Sklan D,Chace DH,Stevens RD,Fridovich I

更新日期：1993-09-01 00:00:00

abstract：:An Escherichia coli strain carrying the recB21 and res-1 mutations showed an abnormally low level of colony-forming ability although it grew essentially normally in liquid medium. The recB21 res-1 strain showed little, if any, of the ultraviolet (UV)-induced deoxyribonucleic acid (DNA) breakdown characteristic of the ...

journal_title：Journal of bacteriology

authors： Kato T

更新日期：1972-12-01 00:00:00

abstract：:Mouse-virulent Salmonella typhimurium strains SR-11 and ATCC 14028-1s express curli fibers, thin aggregative fibers, at ambient temperature on plates as judged by Western blot analysis and electron microscopy. Concomitantly with curli expression, cells develop a rough and dry colony morphology and bind the dye Congo r...

journal_title：Journal of bacteriology

authors： Römling U,Bian Z,Hammar M,Sierralta WD,Normark S

更新日期：1998-02-01 00:00:00

abstract：:In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...

journal_title：Journal of bacteriology

authors： Whitworth DE,Holmes AB,Irvine AG,Hodgson DA,Scanlan DJ

更新日期：2008-03-01 00:00:00

abstract：:We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...

journal_title：Journal of bacteriology

authors： Walderhaug MO,Litwack ED,Epstein W

更新日期：1989-02-01 00:00:00

abstract：:A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization an...

journal_title：Journal of bacteriology

authors： Wu ZL,Charles TC,Wang H,Nester EW

更新日期：1992-04-01 00:00:00

abstract：:We performed a systematic survey of transcription of a large region of the Rhizobium meliloti symbiotic plasmid pSym. This led to the discovery of two new sequences induced during symbiosis. The first sequence was linked to the known nitrogen fixation (nif-fix) gene cluster, and its expression depended on the nifA gen...

journal_title：Journal of bacteriology

authors： David M,Domergue O,Pognonec P,Kahn D

更新日期：1987-05-01 00:00:00

abstract：:The manganese-containing superoxide dismutase (MnSOD) of Vibrio vulnificus, normally detected after the onset of the stationary phase, is expressed during the lag that immediately follows the transfer of cells grown exponentially to a fresh medium acidified to pH 5.0, whereas Fe-containing SOD is constitutively expres...

journal_title：Journal of bacteriology

authors： Kim JS,Sung MH,Kho DH,Lee JK

更新日期：2005-09-01 00:00:00

abstract：:The importance of the physiological state of a culture of Saccharomyces cerevisiae for tolerance to sudden osmotic dehydration was studied, and it was investigated whether specific osmotolerance factors were demonstrable. The microcalorimeter was used to monitor growth, and different physiological states of the cultur...

journal_title：Journal of bacteriology

authors： Blomberg A,Larsson C,Gustafsson L

更新日期：1988-10-01 00:00:00

abstract：:Onoue, Kaoru, (Kyushu University, Fukuoka, Japan), Masayasu Kitagawa, and Yuichi Yamamura. Chemical studies on cellular components of Bordetella pertussis. I. Purification and properties of agglutinogen. J. Bacteriol. 82:648-656. 1961.-A method is described for the preparation of the agglutinogen of Hemophilus pertuss...

journal_title：Journal of bacteriology

authors： ONOUE K,KITAGAWA M,YAMAMURA Y

更新日期：1961-11-01 00:00:00

abstract：:A positive selection method for isolation of nitrogenase-derepressed mutant strains of a filamentous cyanobacterium, Anabaena variabilis, is described. Mutant strains that are resistant to a glutamate analog, L-methionine-D,L-sulfoximine, were screened for their ability to produce and excrete NH4+ into medium. Mutant ...

journal_title：Journal of bacteriology

authors： Spiller H,Latorre C,Hassan ME,Shanmugam KT

更新日期：1986-02-01 00:00:00

abstract：:Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...

journal_title：Journal of bacteriology

authors： Hoffmann HP,Szabo A,Avers CJ

更新日期：1970-10-01 00:00:00

abstract：:The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control elements, we show that the heat shock induction of 32 (out of 56) heat shock proteins is independent of RpoH and HrcA. These...

journal_title：Journal of bacteriology

authors： Rosen R,Büttner K,Becher D,Nakahigashi K,Yura T,Hecker M,Ron EZ

更新日期：2002-03-01 00:00:00

abstract：:Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...

journal_title：Journal of bacteriology

authors： Abriouel H,Benomar N,Pulido RP,Cañamero MM,Gálvez A

更新日期：2011-09-01 00:00:00

abstract：:Different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium Desulfovibrio desulfuricans (Norway strain) have been studied. Two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. This ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six resid...

journal_title：Journal of bacteriology

authors： Bruschi M,Hatchikian CE,Golovleva LA,Gall JL

更新日期：1977-01-01 00:00:00

abstract：:UDP-N-acetylmuramic acid:L-alanine ligase (MurC) catalyzes the addition of the first amino acid to the cytoplasmic precursor of the bacterial cell wall peptidoglycan. The crystal structures of Haemophilus influenzae MurC in complex with its substrate UDP-N-acetylmuramic acid (UNAM) and Mg(2+) and of a fully assembled ...

journal_title：Journal of bacteriology

authors： Mol CD,Brooun A,Dougan DR,Hilgers MT,Tari LW,Wijnands RA,Knuth MW,McRee DE,Swanson RV

更新日期：2003-07-01 00:00:00

abstract：:The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...

journal_title：Journal of bacteriology

authors： Miller KW,Evans RJ,Eisenberg SP,Thompson RC

更新日期：1989-04-01 00:00:00