How many initiator tRNA genes does Escherichia coli need?

abstract：:When Legionella pneumophila grows on agar plates, it secretes a surfactant that promotes flagellum- and pilus-independent "sliding" motility. We isolated three mutants that were defective for surfactant. The first two had mutations in genes predicted to encode cytoplasmic enzymes involved in lipid metabolism. These ge...

journal_title：Journal of bacteriology

authors： Stewart CR,Burnside DM,Cianciotto NP

更新日期：2011-11-01 00:00:00

abstract：:Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

journal_title：Journal of bacteriology

authors： Weickert MJ,Adhya S

更新日期：1993-01-01 00:00:00

abstract：:During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...

journal_title：Journal of bacteriology

authors： Severinova E,Severinov K

更新日期：2006-05-01 00:00:00

abstract：:Expression of the Escherichia coli OmpC and OmpF outer membrane proteins is regulated by the osmolarity of the culture media. In contrast, expression of OmpC in Salmonella typhi is not influenced by osmolarity, while OmpF is regulated as in E. coli. To better understand the lack of osmoregulation of OmpC expression in...

journal_title：Journal of bacteriology

authors： Martínez-Flores I,Cano R,Bustamante VH,Calva E,Puente JL

更新日期：1999-01-01 00:00:00

abstract：UNLABELLED:Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis of the genome of the thermophilic bacterium Alicyclobacillus...

journal_title：Journal of bacteriology

authors： Ding JM,Yu TT,Han NY,Yu JL,Li JJ,Yang YJ,Tang XH,Xu B,Zhou JP,Tang HZ,Huang ZX

更新日期：2015-11-02 00:00:00

abstract：:Sporulation-related repeat (SPOR) domains are small peptidoglycan (PG) binding domains found in thousands of bacterial proteins. The name "SPOR domain" stems from the fact that several early examples came from proteins involved in sporulation, but SPOR domain proteins are quite diverse and contribute to a variety of p...

journal_title：Journal of bacteriology

authors： Yahashiri A,Jorgenson MA,Weiss DS

更新日期：2017-06-27 00:00:00

abstract：:Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been i...

journal_title：Journal of bacteriology

authors： Aoyama K,Mitsubayashi Y,Aiba H,Mizuno T

更新日期：2000-09-01 00:00:00

abstract：:Two proteins that bind penicillin were observed in Escherichia coli infected with lambda phages 141, 142, 650, and 651 from the Kohara genomic library. These phages carry chromosomal DNA fragments that do not contain any known penicillin binding protein (PBP) genes, indicating that unrecognized gene products were exhi...

journal_title：Journal of bacteriology

authors： Henderson TA,Young KD,Denome SA,Elf PK

更新日期：1997-10-01 00:00:00

abstract：:The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing gluco...

journal_title：Journal of bacteriology

authors： Chouayekh H,Nothaft H,Delaunay S,Linder M,Payrastre B,Seghezzi N,Titgemeyer F,Virolle MJ

更新日期：2007-02-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：UNLABELLED:CbrA is an atypical sensor kinase found in Pseudomonas. The autokinase domain is connected to a putative transporter of the sodium/solute symporter family (SSSF). CbrA functions together with its cognate response regulator, CbrB, and plays an important role in nutrient acquisition, including regulation of hu...

journal_title：Journal of bacteriology

authors： Zhang XX,Gauntlett JC,Oldenburg DG,Cook GM,Rainey PB

更新日期：2015-09-01 00:00:00

abstract：:Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) from Acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the Escherichia coli B enzyme. The molecular weights of the two native active enzymes as well as their alpha and beta...

journal_title：Journal of bacteriology

authors： Kleppe RK,Kleppe K

更新日期：1976-02-01 00:00:00

abstract：:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

journal_title：Journal of bacteriology

authors： Jin H,Nikolau BJ

更新日期：2012-10-01 00:00:00

abstract：:Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...

journal_title：Journal of bacteriology

authors： Hase T

更新日期：1983-05-01 00:00:00

abstract：:The cell-wall-less prokaryote Mycoplasma pneumoniae, long considered among the smallest and simplest cells capable of self-replication, has a distinct cellular polarity characterized by the presence of a differentiated terminal organelle which functions in adherence to human respiratory epithelium, gliding motility, a...

journal_title：Journal of bacteriology

authors： Hasselbring BM,Jordan JL,Krause DC

更新日期：2005-09-01 00:00:00

abstract：:Bam35, a temperate double-stranded DNA bacteriophage with a 15-kb linear genome, infects gram-positive Bacillus thuringiensis cells. Bam35 morphology and genome organization resemble those of PRD1, a lytic phage infecting gram-negative bacteria. Bam35 and PRD1 have an outer protein coat surrounding a membrane that enc...

journal_title：Journal of bacteriology

authors： Gaidelyte A,Jaatinen ST,Daugelavicius R,Bamford JK,Bamford DH

更新日期：2005-05-01 00:00:00

abstract：:The stable inheritance of the broad-host-range plasmid RK2 is due at least in part to functions within a region located at coordinates 32.8 to 35.9 kb, termed the RK2 par locus. This locus encodes four previously identified genes in two operons (parCBA and parD; M. Gerlitz, O. Hrabak, and H. Schwab, J. Bacteriol. 172:...

journal_title：Journal of bacteriology

authors： Roberts RC,Helinski DR

更新日期：1992-12-01 00:00:00

abstract：:Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

journal_title：Journal of bacteriology

authors： Calhoun DH,Feary TW

更新日期：1969-01-01 00:00:00

abstract：:In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...

journal_title：Journal of bacteriology

authors： Mills LB,Stanbridge EJ,Sedwick WD,Korn D

更新日期：1977-11-01 00:00:00

abstract：:Escherichia coli fhuF mutants, a sufS::MudI mutant, and a sufD::MudI mutant were found to have the same phenotype: the inability to use ferrioxamine B as an iron source in a plate assay. In addition, the sufS and sufD genes were shown to be regulated by the iron-dependent Fur repressor. Sequence analysis revealed that...

journal_title：Journal of bacteriology

authors： Patzer SI,Hantke K

更新日期：1999-05-01 00:00:00

abstract：:A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, su...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bukald NE

更新日期：1973-01-01 00:00:00

abstract：:Carotovoricin Er is a phage-tail-like bacteriocin produced by Erwinia carotovora subsp. carotovora strain Er, a causative agent for soft rot disease in plants. Here we studied binding and killing spectra of carotovoricin Er preparations for various strains of the bacterium (strains 645Ar, EC-2, N786, and P7) and found...

journal_title：Journal of bacteriology

authors： Nguyen HA,Tomita T,Hirota M,Kaneko J,Hayashi T,Kamio Y

更新日期：2001-11-01 00:00:00

abstract：:Partial purification of a cytochrome bd complex from Azotobacter vinelandii grown under high aeration was achieved by isolating respiratory particles enriched in this hemoprotein via differential centrifugation and detergent extraction. The cytochrome bd complex was subsequently solubilized from the inner membrane wit...

journal_title：Journal of bacteriology

authors： Kolonay JF Jr,Moshiri F,Gennis RB,Kaysser TM,Maier RJ

更新日期：1994-07-01 00:00:00

abstract：:Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...

journal_title：Journal of bacteriology

authors： Sánchez L,Pan W,Viñas M,Nikaido H

更新日期：1997-11-01 00:00:00

abstract：:The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...

journal_title：Journal of bacteriology

authors： Römling U,Duchéne M,Essar DW,Galloway D,Guidi-Rontani C,Hill D,Lazdunski A,Miller RV,Schleifer KH,Smith DW

更新日期：1992-01-01 00:00:00

abstract：:A psychrotolerant marine bacterial strain, designated JUB59(T), was isolated from Antarctic surface seawater and classified as a new species of the genus Bizionia. Here, we present the first draft genome sequence for this genus, which suggests interesting features such as UV resistance, hydrolytic exoenzymes, and nitr...

journal_title：Journal of bacteriology

authors： Lanzarotti E,Pellizza L,Bercovich A,Foti M,Coria SH,Vazquez SC,Ruberto L,Hernández EA,Dias RL,Mac Cormack WP,Cicero DO,Smal C,Nicolas MF,Vasconcelos AT,Marti MA,Turjanski AG

更新日期：2011-12-01 00:00:00

abstract：:The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

更新日期：2004-12-01 00:00:00

abstract：:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...

journal_title：Journal of bacteriology

authors： Gyohda A,Funayama N,Komano T

更新日期：1997-03-01 00:00:00

abstract：:The marRAB operon is one of two operons in the mar locus of Escherichia coli that are divergently transcribed from a central regulatory region, marO. The marRAB operon, transcribed from marOII, controls intrinsic resistance or susceptibility to multiple antibiotics and is inducible by structurally unrelated compounds ...

journal_title：Journal of bacteriology

authors： Seoane AS,Levy SB

更新日期：1995-06-01 00:00:00

abstract：:The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) an...

journal_title：Journal of bacteriology

authors： Narberhaus F,Weiglhofer W,Fischer HM,Hennecke H

更新日期：1996-09-01 00:00:00