Purification and characterization of the cytochrome bd complex from Azotobacter vinelandii: comparison to the complex from Escherichia coli.

Abstract:

:Partial purification of a cytochrome bd complex from Azotobacter vinelandii grown under high aeration was achieved by isolating respiratory particles enriched in this hemoprotein via differential centrifugation and detergent extraction. The cytochrome bd complex was subsequently solubilized from the inner membrane with dodecyl maltoside and purified to near homogeneity via DEAE-Sepharose chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the complex consisted of two subunits, with sizes in good agreement with those predicted from the cloned cyd locus (59.7 and 42 kDa). Spectral analysis of the purified complex indicated that the heme components present were cytochromes b560, b595, and d; CO difference spectral studies identified cytochrome d as a CO-reactive component. The complex had a Km for ubiquinol-1 approximately seven times larger than that for the analogous bd complex from Escherichia coli, and O2 consumption curves revealed a Km value for O2 three times greater than that which we determined for the E. coli bd complex.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kolonay JF Jr,Moshiri F,Gennis RB,Kaysser TM,Maier RJ

doi

10.1128/jb.176.13.4177-4181.1994

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

4177-81

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

杂志文章
  • Genetic analysis of plasmid determinants for microcin J25 production and immunity.

    abstract::Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.12.3661-3663.1996

    authors: Solbiati JO,Ciaccio M,Farías RN,Salomón RA

    更新日期:1996-06-01 00:00:00

  • Effect of growth rate on histidine catabolism and histidase synthesis in Aerobacter aerogenes.

    abstract::A study was made of how the catabolism of a carbon and energy source is affected by the biosynthetic demands of growing bacterial cells. Cultures of Aerobacter aerogenes in l-histidine medium were grown in a chemostat at rates determined by the supply of either sulfate or a required amino acid, l-arginine. It was disc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.131-142.1969

    authors: Jensen DE,Neidhardt FC

    更新日期:1969-04-01 00:00:00

  • Purification and characterization of fimbriae from Salmonella enteritidis.

    abstract::A human isolate of Salmonella enteritidis which displayed strong pellicle formation during static broth culture and mannose-sensitive hemagglutination produced fimbriae which were morphologically indistinguishable from type 1 fimbriae of members of the family Enterobacteriaceae. Fimbrin was purified to homogeneity, an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.1.221-227.1986

    authors: Feutrier J,Kay WW,Trust TJ

    更新日期:1986-10-01 00:00:00

  • A Rhodobacter capsulatus member of a universal permease family imports molybdate and other oxyanions.

    abstract::Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00742-10

    authors: Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

    更新日期:2010-11-01 00:00:00

  • Acetohydroxy acid synthase I is required for isoleucine and valine biosynthesis by Salmonella typhimurium LT2 during growth on acetate or long-chain fatty acids.

    abstract::Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for norma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.917-919.1987

    authors: Dailey FE,Cronan JE Jr,Maloy SR

    更新日期:1987-02-01 00:00:00

  • Enhanced acetohydroxy acid synthase III activity in an ilvH mutant of Escherichia coli K-12.

    abstract::The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5197-5199.1988

    authors: Ricca E,Limauro D,Lago CT,de Felice M

    更新日期:1988-11-01 00:00:00

  • Toxic properties of the cell wall of gram-positive bacteria.

    abstract::The biological activity of Odontomyces viscosus, which has been reported to cause periodontal disease in hamsters, was examined. The microorganism was cultured anaerobically in Brain Heart Infusion broth, and the cells were harvested. The washed cells were injected intradermally into the abdomen of rabbits. After 72 h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.47-52.1967

    authors: Schuster GS,Hayashi JA,Bahn AN

    更新日期:1967-01-01 00:00:00

  • The tryptophanase gene cluster of Haemophilus influenzae type b: evidence for horizontal gene transfer.

    abstract::Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.1.107-118.1998

    authors: Martin K,Morlin G,Smith A,Nordyke A,Eisenstark A,Golomb M

    更新日期:1998-01-01 00:00:00

  • Spontaneous missense mutations in the rplX gene for ribosomal protein L24 from Escherichia coli.

    abstract::Temperature-resistant pseudorevertants of the temperature-sensitive Escherichia coli mutant KNS19, harboring a mutation in rplX, the gene for ribosomal protein L24, were isolated, cloned, and sequenced. The codon GAC for the amino acid Asp in the temperature-sensitive mutant corresponding to position 84 in the protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4854-4856.1987

    authors: Nishi K,Müller M,Schnier J

    更新日期:1987-10-01 00:00:00

  • Methylation Warfare: Interaction of Pneumococcal Bacteriophages with Their Host.

    abstract::Virus-host interactions are regulated by complex coevolutionary dynamics. In Streptococcus pneumoniae, phase-variable type I restriction-modification (R-M) systems are part of the core genome. We hypothesized that the ability of the R-M systems to switch between six target DNA specificities also has a key role in prev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00370-19

    authors: Furi L,Crawford LA,Rangel-Pineros G,Manso AS,De Ste Croix M,Haigh RD,Kwun MJ,Engelsen Fjelland K,Gilfillan GD,Bentley SD,Croucher NJ,Clokie MR,Oggioni MR

    更新日期:2019-09-06 00:00:00

  • A self-splicing group I intron in DNA polymerase genes of T7-like bacteriophages.

    abstract::Group I introns are inserted into genes of a wide variety of bacteriophages of gram-positive bacteria. However, among the phages of enteric and other gram-negative proteobacteria, introns have been encountered only in phage T4 and several of its close relatives. Here we report the insertion of a self-splicing group I ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.23.8153-8155.2004

    authors: Bonocora RP,Shub DA

    更新日期:2004-12-01 00:00:00

  • Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli.

    abstract::Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.564-570.1971

    authors: MacGregor CH,Schnaitman CA

    更新日期:1971-10-01 00:00:00

  • Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System.

    abstract::GspB is a serine-rich glycoprotein adhesin of Streptococcus gordonii that is exported to the bacterial surface by the accessory Sec system. This dedicated export pathway is comprised of seven components (SecA2, SecY2, and five accessory Sec proteins [Asp1 to Asp5]). The latter proteins have no known homologs beyond th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00057-11

    authors: Yen YT,Seepersaud R,Bensing BA,Sullam PM

    更新日期:2011-07-01 00:00:00

  • Cell division mutations in the blue-green bacterium Agmenellum quadruplicatum strain BG1: a comparison of the cell wall.

    abstract::The peptidoglycan from two types of filamentous cell division mutants of Agmenellum quadruplicatum strain BG1 has been compared to that of the parent organism. Small variations in the total peptidoglycan composition on a dry-weight basis were found in the mutants. The reduced level of peptidoglycan in the serpentine m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.2.614-621.1972

    authors: Ingram LO,Van Baalen C,Fisher WD

    更新日期:1972-08-01 00:00:00

  • Active subunits of Escherichia coli glutamate synthase.

    abstract::The large and small subunits of Escherichia coli glutamate synthase were isolated. The small subunit catalyzes the NH(3)-dependent synthesis of glutamate. The large subunit exhibits glutaminase activity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.1.539-541.1976

    authors: Mantsala P,Zalkin H

    更新日期:1976-04-01 00:00:00

  • The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro.

    abstract::Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.14.3808-3814.2002

    authors: Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

    更新日期:2002-07-01 00:00:00

  • Genome sequence of Yersinia pestis KIM.

    abstract::We present the complete genome sequence of Yersinia pestis KIM, the etiologic agent of bubonic and pneumonic plague. The strain KIM, biovar Mediaevalis, is associated with the second pandemic, including the Black Death. The 4.6-Mb genome encodes 4,198 open reading frames (ORFs). The origin, terminus, and most genes en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.16.4601-4611.2002

    authors: Deng W,Burland V,Plunkett G 3rd,Boutin A,Mayhew GF,Liss P,Perna NT,Rose DJ,Mau B,Zhou S,Schwartz DC,Fetherston JD,Lindler LE,Brubaker RR,Plano GV,Straley SC,McDonough KA,Nilles ML,Matson JS,Blattner FR,Perry RD

    更新日期:2002-08-01 00:00:00

  • CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F' INCORPORATING THEM.

    abstract::Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.554-561.1964

    authors: ISHIBASHI M,SUGINO Y,HIROTA Y

    更新日期:1964-03-01 00:00:00

  • Purification and partial characterization of a Nocardia brasiliensis extracellular protease.

    abstract::Nocardia brasiliensis possess proteolytic activities that can be readily detected in a variety of media. In a modified formulation of a growth medium originally used for Streptomyces aureofaciens, N. brasiliensis was found to secrete proteolytic enzymes, one of which was capable of hydrolyzing casein. This enzyme was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.2.627-631.1984

    authors: Zlotnik H,Schramm VL,Buckley HR

    更新日期:1984-02-01 00:00:00

  • Proteus mirabilis mutants defective in swarmer cell differentiation and multicellular behavior.

    abstract::Proteus mirabilis is a dimorphic bacterium which exists in liquid cultures as a 1.5- to 2.0-microns motile swimmer cell possessing 6 to 10 peritrichous flagella. When swimmer cells are placed on a surface, they differentiate by a combination of events that ultimately produce a swarmer cell. Unlike the swimmer cell, th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6279-6288.1991

    authors: Belas R,Erskine D,Flaherty D

    更新日期:1991-10-01 00:00:00

  • Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon.

    abstract::The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.5.1213-1220.1993

    authors: Mattern SG,Brawner ME,Westpheling J

    更新日期:1993-03-01 00:00:00

  • Identification, characterization, and functional analysis of a gene encoding the ferric uptake regulation protein in Bartonella species.

    abstract::Environmental iron concentrations coordinately regulate transcription of genes involved in iron acquisition and virulence via the ferric uptake regulation (fur) system. We identified and sequenced the fur gene and flanking regions of three Bartonella species. The most notable difference between Bartonella Fur and othe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.19.5751-5755.2001

    authors: Park SY,Kelminson KL,Lee AK,Zhang P,Warner RE,Rehkopf DH,Calderwood SB,Koehler JE

    更新日期:2001-10-01 00:00:00

  • SepL resembles an aberrant effector in binding to a class 1 type III secretion chaperone and carrying an N-terminal secretion signal.

    abstract::Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00760-10

    authors: Younis R,Bingle LE,Rollauer S,Munera D,Busby SJ,Johnson S,Deane JE,Lea SM,Frankel G,Pallen MJ

    更新日期:2010-11-01 00:00:00

  • General method, using Mu-Mud1 dilysogens, to determine the direction of transcription of and generate deletions in the glnA region of Escherichia coli.

    abstract::A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.260-268.1981

    authors: MacNeil D

    更新日期:1981-04-01 00:00:00

  • Flagellar assembly in Salmonella typhimurium: analysis with temperature-sensitive mutants.

    abstract::The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.3.1327-1339.1990

    authors: Jones CJ,Macnab RM

    更新日期:1990-03-01 00:00:00

  • Effects of a mutator mutation of Salmonella typhimurium on P22 and R factor genes.

    abstract::A mutator mutation of Salmonella typhimurium, observed in a strain resistant to P22 and rifampin, affects mutation rates of bacterial, R factor, and prophage P22 genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.638-639.1974

    authors: Guterman SK,Wright A

    更新日期:1974-08-01 00:00:00

  • Genome sequence of the agar-degrading marine bacterium Alteromonadaceae sp. strain G7.

    abstract::Here, we present the high-quality draft genome sequence of the agar-degrading marine gammaproteobacterium Alteromonadaceae sp. strain G7, which was isolated from coastal seawater to be utilized as a bioresource for production of agar-derived biofuels. The 3.91-Mb genome contains a number of genes encoding algal polysa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01931-12

    authors: Kwak MJ,Song JY,Kim BK,Chi WJ,Kwon SK,Choi S,Chang YK,Hong SK,Kim JF

    更新日期:2012-12-01 00:00:00

  • Continuous culture of Rhodotorula rubra: kinetics of phosphate-arsenate uptake, inhibition, and phosphate-limited growth.

    abstract::The pink yeast Rhodotorula rubra of marine origin was found to be capable of extended growth at very low phosphate concentrations (K(0.5) = 10.8 nm). Average intracellular phosphate concentrations, based on isotope exchange techniques, were 15 to 200 nm, giving concentration gradients across the cell envelope of about...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.599-611.1973

    authors: Button DK,Dunker SS,Morse ML

    更新日期:1973-02-01 00:00:00

  • Oncoupling of protein and ribonucleic acid synthesis by 5',5',5'-trifluoroleucine in Salmonella typhimurium.

    abstract::The addition of 5',5',5'-trifluoroleucine (fluoroleucine) to leucine auxotrophs of Salmonella typhimurium permitted protein but not ribonucleic acid (RNA) synthesis to continue after leucine depletion. The uncoupling of the formation of these macromolecules by fluoroleucine was apparent if RNA and protein synthesis wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.107-112.1969

    authors: Trela JM,Freundlich M

    更新日期:1969-07-01 00:00:00

  • Isolation and characterization of spontaneously occurring TOL plasmid mutants of Pseudomonas putida HS1.

    abstract::A strain of Pseudomonas (P. putida HS1) was found to resemble P. putida (arvilla) mt-2 in its ability to degrade toluene, m- and p-xylene, 1,2,4-trimethylbenzene (pseudocumene), and 3-ethyltoluene via oxidation of a methyl substituent and reactions of the meta-fission pathway. The ability to degrade these substrates b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.3.952-964.1981

    authors: Kunz DA,Chapman PJ

    更新日期:1981-06-01 00:00:00