Spontaneous missense mutations in the rplX gene for ribosomal protein L24 from Escherichia coli.

abstract：:A 16-kb BamHI fragment of the lactose plasmid pNZ63 from Leuconostoc lactis NZ6009 was cloned in Escherichia coli MC1061 by using pACYC184 and was found to express a functional beta-galactosidase. Deletion and complementation analysis showed that the coding region for beta-galactosidase was located on a 5.8-kb SalI-Ba...

journal_title：Journal of bacteriology

authors： David S,Stevens H,van Riel M,Simons G,de Vos WM

更新日期：1992-07-01 00:00:00

abstract：:A method of assay of immunogenic potency of the cholera gut toxin is described; it is based on the relation of dose of antigen to neutralizing antibody titer produced in the rabbit under defined conditions and allows quantification of immunogenicity as immunogenic units per milligram of protein. Evidence, based on imm...

journal_title：Journal of bacteriology

authors： Kaur J,Burrows W,Cercavski L

更新日期：1969-11-01 00:00:00

abstract：:Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli...

journal_title：Journal of bacteriology

authors： Hanzelka BL,Greenberg EP

更新日期：1996-09-01 00:00:00

abstract：:In recent work, we described the excision of a large genomic region from Enterococcus faecium D344R in which the sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of in...

journal_title：Journal of bacteriology

authors： Rice LB,Carias LL,Hutton-Thomas R,Rudin S

更新日期：2007-05-01 00:00:00

abstract：:Studies with cell-free protein preparations from a series of recombinant strains of Pseudomonas denitrificans demonstrated that precorrin-3 is converted into a further trimethylated intermediate, named precorrin-3B, along the pathway to coenzyme B12. It was then shown that the part of the pathway from precorrin-3 (cal...

journal_title：Journal of bacteriology

authors： Debussche L,Thibaut D,Cameron B,Crouzet J,Blanche F

更新日期：1993-11-01 00:00:00

abstract：:Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...

journal_title：Journal of bacteriology

authors： Quinlan DC,Maniloff J

更新日期：1973-07-01 00:00:00

abstract：:The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

journal_title：Journal of bacteriology

authors： Mattern SG,Brawner ME,Westpheling J

更新日期：1993-03-01 00:00:00

abstract：:Relatively high transfo mation frequencies were obtained with Micrococcus lysodeikticus cells, previou[ill]sly grown in defined broth, with a tris(hydroxymethyl)-aminomethane-glutamate b uffer containing Mg(++), Ca(++), Ba(++), or Sr(++). ...

journal_title：Journal of bacteriology

authors： Kloos WE

更新日期：1969-06-01 00:00:00

abstract：:We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

journal_title：Journal of bacteriology

authors： Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

更新日期：1996-06-01 00:00:00

abstract：:Escherichia coli mutants lacking activities of all known cytosolic ATP-dependent proteases (Lon, ClpAP, ClpXP, and HslVU), due to double deletions [DeltahslVU and Delta(clpPX-lon)], cannot grow at low (30 degrees C) or very high (45 degrees C) temperatures, unlike those carrying either of the deletions. Such growth de...

journal_title：Journal of bacteriology

authors： Kanemori M,Yanagi H,Yura T

更新日期：1999-06-01 00:00:00

abstract：:L. Sibold, M. Henriquet, O. Possot, and J.-P. Aubert (Res. Microbiol. 142:5-12, 1991) cloned and sequenced two nifH-homologous open reading frames (ORFs) from Methanosarcina barkeri 227. Phylogenetic analysis of the deduced amino acid sequences of the nifH ORFs from M. barkeri showed that nifH1 clusters with nifH gene...

journal_title：Journal of bacteriology

authors： Chien YT,Zinder SH

更新日期：1994-11-01 00:00:00

abstract：:The halophilic bacterium Halomonas elongata synthesizes as its main compatible solute the aspartate derivative ectoine. We constructed a deletion mutant of H. elongata, KB1, defective in ectoine synthesis and tolerating elevated salt concentrations only in the presence of external compatible solutes. The dependency of...

journal_title：Journal of bacteriology

authors： Grammann K,Volke A,Kunte HJ

更新日期：2002-06-01 00:00:00

abstract：UNLABELLED:The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo Although attempts have been made to understand the function of Erp protein, its exact ro...

journal_title：Journal of bacteriology

authors： Ganaie AA,Trivedi G,Kaur A,Jha SS,Anand S,Rana V,Singh A,Kumar S,Sharma C

更新日期：2016-09-22 00:00:00

abstract：:The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...

journal_title：Journal of bacteriology

authors： Choi KY,Zalkin H

更新日期：1992-10-01 00:00:00

abstract：:A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodec...

journal_title：Journal of bacteriology

authors： Hancock RE,Carey AM

更新日期：1979-12-01 00:00:00

abstract：:The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...

journal_title：Journal of bacteriology

authors： Winkler HH

更新日期：1984-01-01 00:00:00

abstract：:Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA ...

journal_title：Journal of bacteriology

authors： Ward MJ,Lew H,Zusman DR

更新日期：2000-01-01 00:00:00

abstract：:We described previously a class of mutations (phenotypic designation R2(-)) in Staphylococcus aureus that were unlinked to penicillinase genes but nevertheless imposed constitutive synthesis of this enzyme upon wild-type plasmids. The plasmids affected by these mutations have been found to possess the same maintenance...

journal_title：Journal of bacteriology

authors： Cohen S,Vernon EG,Sweeney HM

更新日期：1970-09-01 00:00:00

abstract：:Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

journal_title：Journal of bacteriology

authors： Lobanovska M,Tang CM,Exley RM

更新日期：2019-09-20 00:00:00

abstract：:For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

journal_title：Journal of bacteriology

authors： White TC,Agabian N

更新日期：1995-09-01 00:00:00

abstract：:The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...

journal_title：Journal of bacteriology

authors： Pihl TD,Sharma S,Reeve JN

更新日期：1994-10-01 00:00:00

abstract：:The thermosensitive growth phenotype exerted by runaway-mutant plasmids was suppressed by sublethal doses of the DNA gyrase inhibitors novobiocin or nalidixic acid, although the latter drug was less efficient. A novobiocin-resistant gyrB mutant Escherichia coli strain prevented expression of the runaway phenotype at 3...

journal_title：Journal of bacteriology

authors： Uhlin BE,Nordström K

更新日期：1985-05-01 00:00:00

abstract：:Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells...

journal_title：Journal of bacteriology

authors： Chédin F,Dervyn R,Ehrlich SD,Noirot P

更新日期：1997-02-01 00:00:00

abstract：:Normal animal sera inhibit at least one Clostridium histolyticum proteinase. An assay procedure based on immune hemolysis was developed for the estimation of this inhibition. This inhibitory activity occurs in various levels in the sera of different animal species. The highest titers have been obtained with rat sera. ...

journal_title：Journal of bacteriology

authors： Luzzati A,Goldlust MB,Levine L

更新日期：1968-12-01 00:00:00

abstract：:Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...

journal_title：Journal of bacteriology

authors： Roggiani M,Hahn J,Dubnau D

更新日期：1990-07-01 00:00:00

abstract：:A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

journal_title：Journal of bacteriology

authors： Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

更新日期：1987-10-01 00:00:00

abstract：UNLABELLED:Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for thymineless death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the...

journal_title：Journal of bacteriology

authors： Itsko M,Schaaper RM

更新日期：2016-05-13 00:00:00

abstract：:Vibrio alginolyticus contained two adjacent genes, ktrA and ktrB, which encode a new type of bacterial K(+)-uptake system. KtrA and KtrB are peripheral and integral membrane proteins, respectively. Six of the nine sequenced bacterial genomes contain homologs to both ktrA and ktrB, suggesting that KtrAB is widespread. ...

journal_title：Journal of bacteriology

authors： Nakamura T,Yuda R,Unemoto T,Bakker EP

更新日期：1998-07-01 00:00:00

abstract：:Large amounts of sigA mRNA, encoding the primary sigma factor (PSF) in Synechocystis sp. strain PCC 6803, accumulated under standard growth conditions, while stress conditions like heat or high salinity led to a rapid decrease in sigA mRNA content. The sigB, sigC, sigD, and sigE genes, encoding PSF-like sigma factors,...

journal_title：Journal of bacteriology

authors： Tuominen I,Tyystjärvi E,Tyystjärvi T

更新日期：2003-02-01 00:00:00

abstract：:The ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobU in Salmonella enterica). The latter activity is required for cobinamide salvaging in bacteria. This paper reports evidence that ar...

journal_title：Journal of bacteriology

authors： Woodson JD,Zayas CL,Escalante-Semerena JC

更新日期：2003-12-01 00:00:00