Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon.

Abstract:

:The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and that resulted in deregulated expression. Certain combinations of base changes within a series of hexamers that lie within two pairs of direct repeat sequences resulted in significant expression from galP1 in the absence of inducer. These motifs are further implicated in regulation by the observation that DNA fragments containing the hexamers and direct repeat sequences resulted in increased transcription from the chromosomal copy of galP1 on multicopy plasmids in the absence of galactose. We suggest that these hexamers and direct repeat sequences constitute an operator for the negative regulation of the Streptomyces gal operon.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mattern SG,Brawner ME,Westpheling J

doi

10.1128/jb.175.5.1213-1220.1993

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

1213-20

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

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